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Quantitative kinetics of glucose appearance and disposal following a 13C-labelled starch-rich meal: comparison of male and female subjects

Published online by Cambridge University Press:  09 March 2007

M. Denise Robertson*
Affiliation:
Human Nutrition Research Centre, Dept Biological and Nutritional Sciences, University of Newcastle, Newcastle-upon-Tyne NE1 7RU, UK
Geoff Livesey
Affiliation:
Independent Nutrition Logic, Pealerswell House, Wymondham, Norfolk NR18 0QX, UK
John C. Mathers
Affiliation:
Human Nutrition Research Centre, Dept Biological and Nutritional Sciences, University of Newcastle, Newcastle-upon-Tyne NE1 7RU, UK
*
*Corresponding author:Dr M. Denise Robertson, fax +44 865 224652, email denise.robertson@oxlip.ox.ac.uk
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Abstract

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In the UK, starch contributes up to 25 % of energy intake in adults (). The present study investigated the acute response to a starchy meal on whole-body glucose metabolism and assessed insulin sensitivity in men compared with women. Low insulin sensitivity has been postulated to pre-dispose individuals to a cluster of associated abnormalities known to increase the risk of CHD. Metabolic responses to a 13C-labelled meal were determined in conjunction with a primed continuous infusion of D-[6,6-2H]glucose in groups of healthy age- and BMI-matched men and women. Peripheral plasma glucose disposal (Gd) was computed using non-steady state kinetics in a single compartment model, simultaneously with determination of whole-body net glucose oxidation by indirect calorimetry. Insulin sensitivity was derived using cumulative Gd as the dependent variable, and time and the integrated insulin concentration as independent variables. The female group had the higher fractional rate of glucose appearance in plasma from starch (P=0·019) immediately after ingestion. Females also had a higher rate of plasma Gd and a significantly higher insulin-dependent Gd (6·8 v. 5·6 μg glucose/(min.kg) per pmol insulin, P<0·05) compared with the males. A smaller absolute pool of endogenous glucose in females allowed the rate of exogenous 13CO2 production to be significantly higher in the females (P=0·007) corresponding also to a significantly higher (P<0·05) carbohydrate oxidation rate obtained by indirect calorimetry. The present study suggests that during the ingestion of a starchy meal, females exhibit higher glucose flux and greater whole-body insulin sensitivity than males.

Type
Research Article
Copyright
Copyright © The Nutrition Society 2002

References

Boirie, Y, Gachon, P, Corny, S, Fauquant, J, Maubois, JL & Beaufrere, B (1996) Acute postprandial changes in leucine metabolism as assessed with an intrinsically labeled milk protein. American Journal of Physiology 271, E1083E1091.Google ScholarPubMed
Capaldo, B, Gastaldelli, A, Antoniello, S, Auletta, M, Pardo, F, Ciociaro, D, Guida, R, Ferrannini, E & Sacca, L (1999) Splanchnic and leg substrate exchange after ingestion of a natural mixed meal in humans. Diabetes 48, 958966.CrossRefGoogle ScholarPubMed
Delarue, J, Normand, S, Pachiaudi, C, Beylot, M, Lamisse, F & Riou, JP (1993) The contribution of naturally labelled 13C fructose to glucose appearance in humans. Diabetologia 36, 338345.CrossRefGoogle ScholarPubMed
Donahue, RP, Prineas, RJ, DeCarlo Donahue, R & Skyler, JS (1996) The female 'insulin advantage' in a biracial cohort: Results from the Miami Community Health Study. International Journal of Obesity 20, 7682.Google Scholar
Elia, M & Livesey, G (1992) Energy expenditure and fuel selection in biological systems: the theory and practice of calculations based on indirect calorimetry and tracer methods. In Metabolic Control of Eating, Energy Expenditure and the Bioenergetics of Obesity, pp. 68131 [Simopoulos, AP, editor]. Basel: Karger.Google Scholar
Englyst, KN, Englyst, HN, Hudson, GJ, Cole, TJ & Cummings, JH (1999) Rapidly available glucose in foods: an in vitro measurement that reflects the glycemic response. American Journal of Clinical Nutrition 69, 448454.CrossRefGoogle Scholar
Faulks, RM, Roe, MA & Livesey, G (1994) Production of 13C labelled peas for studies of starch metabolism. In EURESTA: European Commission – Science, Research and Development, [Asp, N-G, Van, JMMAmelsvoort, and Hautvast, JGAJ, editors], EURESTA.Google Scholar
Gregory, J, Foster, K, Tyler, H & Wiseman, M (1990) The Dietary and Nutritional Survey of British Adults. London: H.M. Stationery Office.Google Scholar
Hampton, SM (1984) C-peptide of pro-insulin: its diagnostic use and a possible physiological role. PhD Thesis, University of Survey.Google Scholar
Kahn, SE, Prigeon, RL, McCulloch, DK, Boyko, EJ, Bergman, RN, Schwartz, MW, Neifing, JL, Ward, WK, Beard, JC, Palmer, JP & Porte, D Jr (1994) The contribution of insulin-dependent and insulin-independent glucose uptake to intravenous glucose tolerance in healthy human subjects. Diabetes 43, 587592.CrossRefGoogle ScholarPubMed
Kannel, WB & Abbott, RD (1987) Incidence and prognosis of myocardial infarction in women: the Framingham study. In Coronary Heart Disease in Women, pp. 208214 [Eaker, ED, Packard, B, Wenger, NK, Clarkson, TB and Tyroler, HA, editors]. New York, NY: Haymarket-Doyma.Google ScholarPubMed
Leijssen, DPC & Elia, M (1996) Recovery of 13CO2 and 14CO2 in human bicarbonate studies: a critical review with original data. Clinical Science 91, 665677.CrossRefGoogle ScholarPubMed
Livesey, G, Wilson, PDG, Dainty, JR, Brown, JC, Faulks, RM, Roe, MA, Newman, TA, Eagles, J, Mellon, FA & Greenwood, RH (1998) Simultaneous time-varying systematic appearance of oral and hepatic glucose in adults monitored with stable isotopes. American Journal of Physiology 275, E717E728.Google Scholar
Mathers, JC & Daly, ME (2001) Food polysaccharides, glucose absorption and insulin sensitivity. In Advanced Dietary Fibre Technology, pp. 186197 [McCleary, B and Prosky, L, editors]. Oxford: Blackwell Science.Google Scholar
Nilsson, PM, Lind, L, Pollare, T, Berne, C & Lithell, H (2000) Differences in insulin sensitivity and risk markers due to gender and age in hypertensives. Journal of Human Hypertension 14, 5156.CrossRefGoogle ScholarPubMed
Noah, L, Krempe, M, Lecannu, G, Maugere, P & Champ, M (2000) Bioavailability of starch and postprandial changes in splanchnic glucose metabolism in pigs. American Journal of Physiology 278, E181E188.Google ScholarPubMed
Normand, S, Pachiaudi, C, Khalfallah, Y, Guilluy, R, Mornex, R & Riou, JP (1992) 13C appearance in plasma glucose and breath CO2 during feeding with naturally enriched 13C-enriched starchy food in normal humans. American Journal of Clinical Nutrition 55, 430435.CrossRefGoogle ScholarPubMed
Nuutila, P, Knuuti, MJ, Maki, M, Laine, H, Ruotsalainen, U, Teras, M, Haaparanta, M, Solin, O & Yki-Jarvinen, H (1995) Gender and insulin sensitivity in the heart and in skeletal muscles: studies using positron emission tomography. Diabetes 44, 3136.CrossRefGoogle ScholarPubMed
Pickert, A, Overkamp, D, Renn, W, Liebich, H & Eggstein, M (1991) Selected ion monitoring gas chromatography/mass spectrometry using uniformly labeled 13C glucose for determination of glucose turnover in man. Biological Mass Spectrometry 20, 203209.CrossRefGoogle ScholarPubMed
Reaven, GM (1988) Role of insulin resistance in human disease. Diabetes 31, 670673.CrossRefGoogle Scholar
Robertson, MD (1997) Starch absorption and glucose kinetics in normal and colectomized humans. PhD Thesis, University of Newcastle.Google Scholar
Robertson, MD, Livesey, G, Hampton, SM & Mathers, JC (2000) Evidence for altered control of glucose disposal after total colectomy. British Journal of Nutrition 84, 813819.CrossRefGoogle ScholarPubMed
Robertson, MD, Mathers, JC, Mishra, V & Livesey, G (1996) Is the ileostomist a good model for studies of starch digestion in man? Proceedings of the Nutrition Society 55, 50A.Google Scholar
Schrauwen, P, Blaak, EE, Van, Aggel Leijssen, DPC, Borghouts, LB & Wagenmakers, AJM (2000) Determinants of the acetate recovery factor: Implications for estimation of 13C substrate oxidation. Clinical Science 98, 587592.CrossRefGoogle ScholarPubMed
Steele, R (1959) Influence of glucose loading and of injected insulin on hepatic glucose output. Annals of the New York Academy of Sciences 82, 420430.CrossRefGoogle ScholarPubMed
Tissot, S, Normand, S, Guilluy, R, Pachiaudi, C, Beylot, M, Laville, M, Cohen, R, Mornex, R & Riou, JP (1990) Use of a new gas chromatograph isotope ratio spectrometer to trace exogenous 13C-labelled glucose at a very low level of enrichment in man. Diabetologia 33, 449456.CrossRefGoogle Scholar
Van-Hall, G (1999) Correction factors for 13C labelled substrate oxidation at whole-body and muscle level. Proceedings of the Nutrition Society 58, 979986.CrossRefGoogle ScholarPubMed
Wieko, J & Shernam, WR (1976) Boroacetylation of carbohydrates. Correlations between structure and mass spectral behaviour inmonoacetylhexose cyclic boronic esters. Journal of the American Chemical Society 98, 76317637.CrossRefGoogle Scholar
Yip, J, Facchini, FS & Reaven, GM (1998) Resistance to insulin-mediated glucose disposal as a predictor of cardiovascular disease. Journal of Clinical Endocrinology and Metabolism 83, 27732776.CrossRefGoogle ScholarPubMed