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The rat as a model for pigs: comparative values for the digestibility of NSP and other macronutrients

Published online by Cambridge University Press:  09 March 2007

Elisabeth Wisker  and
Knud Erik Bach Knudsen
Elisabeth Wisker
Affiliation:
Christian Albrechts-University of Kiel, Institute of Human Nutrition and Food Science, D-24105 Kiel, Germany
Knud Erik Bach Knudsen*
Affiliation:
Danish Institute of Agricultural Sciences, Department of Animal Nutrition and Physiology, Research Centre Foulum, PO Box 50, DK-8830 Tjele, Denmark
*
*Corresponding author: Research Professor Knud Erik Bach Knudsen, fax +45 89 99 13 78, email KnudErik.Bachknudsen@agrsci.dk
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Abstract

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The present investigation was undertaken to study whether conventional male Wistar rats could be used as a model for pigs with regard to total tract digestibilities of NSP and macronutrients and whether nebacitin-treated rats could be used as a model for small intestinal digestibility in pigs. Nineteen experimental diets prepared from different fractions of wheat and oats, and which all had been evaluated in experiments with ileal cannulated pigs, were used for the present study. There was a close relationship between the total tract digestibilities of organic matter in the two species. The same was the case with regard to the digestibility of total NSP and arabinoxylans, but the values were on average 6 % lower in rats than in pigs. On average, there were no significant differences between rats and pigs with regard to faecal protein digestibility. However, protein in oat-based diets was significantly better digested in the rat than the pig. The digestibility of fat was consistently higher in rats than in pigs, with the biggest difference being found in oat-based diets, in which most of the fat was locked in cell structures. For the wheat-based diets, in which a large proportion of the fat was present as added fat, there was a greater similarity between the two species. In nebacitin-treated rats the digestibility of organic matter, starch, protein and fat was negatively related to the dietary level of NSP, but this model could not be used to predict the small intestinal digestibility of NSP and macronutrients in ileal-cannulated pigs.

Keywords

DigestibilityNon-starch polysaccharidesProteinFatPigsRats
Type
Research Article
Information
British Journal of Nutrition , Volume 90 , Issue 2 , August 2003 , pp. 373 - 383
Copyright
Copyright © The Nutrition Society 2003

References

Bach Knudsen, KE (1991) Methodological aspects of in vivo models for measuring of starch digestibility: animal models. In Methodological Aspects of in vivo Models for Measuring of Starch Digestibility. Report of a European Flair Concerted Action Workshop. pp. 40–54. [Gudmand-Høyer, E, editor]. Copenhagen: Euresta.Google Scholar
Bach Knudsen, KE (1997) Carbohydrate and lignin contents of plant materials used in animal feeding. Anim Feed Sci Technol 67, 319–338.CrossRefGoogle Scholar
Bach Knudsen, KE (2001) The nutritional significance of "dietary fibre" analysis. Anim Feed Sci Technol 90, 3–20.CrossRefGoogle Scholar
Bach Knudsen, KE, Agergaard, N & Olesen, HP (1991) Effect of caecectomy and transit time on digestibility of plant polysaccharides and of amino acid in rats. J Anim Physiol Anim Nutr 66, 190–203.CrossRefGoogle Scholar
Bach Knudsen, KE Åman P & Eggum, BO (1987) Nutritive value of Danish-grown barley varieties, I, Carbohydrates and other major constituents. J Cereal Sci 6, 173–186.CrossRefGoogle Scholar
Bach Knudsen, KE & Canibe, N (2000) Breakdown of plant carbohydrates in the digestive tract of pigs fed on wheat- or oat-based rolls. J Sci Food Agric 80, 1253–1261.3.0.CO;2-0>CrossRefGoogle Scholar
Bach Knudsen, KE & Hansen, I (1991) Gastrointestinal implications in pigs of wheat and oat fractions. 1. Digestibility and bulking properties of polysaccharides and other major constituents. Br J Nutr 65, 217–232.CrossRefGoogle ScholarPubMed
Bach Knudsen, KE, Jensen, BB & Hansen, I (1993) Digestion of polysaccharides and other major components in the small and large intestine of pigs fed on diets consisting of oat fractions rich in beta-D-glucan. Br J Nutr 70, 537–556.CrossRefGoogle Scholar
Bach Knudsen, KE, Jensen, BB & Hansen, I (1993) Oat bran but not a beta glucan enriched oat fraction enhances butyrate production in the large intestine of pigs. J Nutr 123, 1235–1247.CrossRefGoogle Scholar
Bach Knudsen, KE & Jørgensen, H (2001) Intestinal degradation of carbohydrates from birth to maturity. In Digestive Physiology in Pigs. pp. 109–120. [Lindberg, JE and Ogle, B, editors]. Wallingford, Oxon.: CABI Publishing.Google Scholar
Bach Knudsen, KE, Wisker, E, Daniel, M, Feldheim, W & Eggum, BO (1994) Digestibility of energy, protein, fat and non-starch polysaccharides in mixed diets: comparative studies between man and the rat. Br J Nutr 71, 471–487.CrossRefGoogle ScholarPubMed
Beames, RM, Helm, JH, Eggum, BO, Boisen, S, Bach Knudsen, KE & Swift, ML (1996) A comparison of methods for measuring the nutritive value for pigs of a range of hulled and hulless barley cultivars. Anim Feed Sci Technol 62, 189–201.CrossRefGoogle Scholar
Björck, I, Nyman, M, Pedersen, B, Siljestöm, M, Asp, N-G & Eggum, BO (1986) On the digestibility of starch in wheat bread – studies in vitro and in vivo. J Cereal Sci 4, 1–11.CrossRefGoogle Scholar
Björnhag, G (1992) Anatomy of the digestive tract and transport of digesta. In The Rat as a Model for Man and Pig in Nutritional and Physiological Studies. Proceedings. pp. 9–11. [Jungvid, H, Forshell, LP & Eggum, BO, editors]. Lidköping: Gramineer AB.Google Scholar
Boisen, S, Agergaard, N, Rotenberg, S & Kragelund, Z (1985) Effects of gut flora on intestinal activities of trypsin, chymotrypsin, elastase and amylase in growing rats fed purified diets with cellulose, pectin or sand. Z Tierphysiol Tierernähr Futtermittelkd 53, 245–254.CrossRefGoogle Scholar
Brunsgaard, G, Bach Knudsen, KE & Eggum, BO (1995) The influence of the period of adaptation on the digestibility of diets containing different types of indigestible polysaccharides in rats. Brit J Nutr 74, 833–848.Google ScholarPubMed
Canibe, N, Bach Knudsen, KE & Eggum, BO (1997) Digestibility and nitrogen balance in rats given dried or toasted peas (Pisum sativum) of different years of harvest. J Sci Food Agric 73, 21–33.3.0.CO;2-L>CrossRefGoogle Scholar
Donkoh, A, Moughan, PJ & Smith, WC (1994) The laboratory rat as a model animal for determining ileal amino acid digestibility in meat and bone meal for the growing pig. Anim Feed Sci Technol 49, 57–71.CrossRefGoogle Scholar
Eggum, BO (1973) In A Study of Certain Factors Influencing Protein Utilization in Rats and Pigs. Report no. 406. pp. 177Copenhagen: National Institute of Animal Science.Google Scholar
Eggum, BO & Beames, RM (1986) Use of laboratory animals as models for studies on nutrition of domestic animals. In Laboratory Animals. pp. 265–290. [Ruitenberg, EJ and Peters, PWJ, editors]. Amsterdam: Elsevier Science Publishers BV.Google Scholar
Eggum, BO, Fekadu, M, Wolstrup, J, Sauer, WC & Just, A (1979) The effect of dietary antibiotics on protein and energy metabolism in rats: possible significance of the gut microflora. J Sci Food Agric 130, 177–184.CrossRefGoogle Scholar
Eggum, BO, Thorbek, G, Beames, RM, Chwalibog, A & Henckel, S (1982) Influence of diet and microbial activity in the digestive tract on digestibility, and nitrogen and energy metabolism in rats and pigs. Brit J Nutr 48, 161–175.CrossRefGoogle ScholarPubMed
Englyst, HN, Wiggins, HS & Cummings, JH (1982) Determination of non-starch polysaccharides in plant foods by gas-liquid chromatography of constituent sugars as alditol acetates. Analyst 107, 307–318.CrossRefGoogle ScholarPubMed
Goodlad, JS & Mathers, JC (1991) Digestion by pigs of non-starch polysaccharides in wheat and raw peas (Pisum sativum) fed in mixed diets. Br J Nutr 65, 259–270.CrossRefGoogle ScholarPubMed
Jakobsen, K (1992) Digestibility of fat and fatty acids in rat, pig and man. In The Rat as a Model for Man and Pig in Nutritional and Physiological Studies. Proceedings. pp. 32–48. [Jungvid, H, Forshell, LP & Eggum, BO, editors]. Lidköping: Gramineer AB.Google Scholar
Jensen, BB (1992) Microbial activity in various segments of the gastrointestinal tract of rat, pig and man. In The Rat as a Model for Man and Pig in Nutritional and Physiological Studies. Proceedings. pp. 24–31. [Jungvid, H, Forshell, LP & Eggum, BO, editors]. Lidköping: Gramineer AB.Google Scholar
Johansen, HN, Bach Knudsen, KE, Wood, PJ & Fulcher, RG (1997) Physico-chemical properties and the digestibility of polysaccharides from oats in the gastrointestinal tract of pigs. J Sci Food Agric 73, 81–92.3.0.CO;2-Z>CrossRefGoogle Scholar
Jørgensen, KG & Aastrup, S (1987) Determination of ß-glucan in barley, malt, wort and beer. In Modern Methods of Plant Analysis. pp. 88–108. [Linskens, HF and Jackson, JF, editors]. Berlin: Springer–Verlag.Google Scholar
Just, A, Jørgensen, H & Fernandez, JA (1983) Maintenance requirement and the energy value of different diets for growth in pigs. Livest Prod Sci 10, 487–506.CrossRefGoogle Scholar
Larsen, T, Ostergard, K, Hansen, I, Bach Knudsen, KE & Eggum, BO (1991) Daily food intake and digestibility in rats. Br J Nutr 65, 29–35.CrossRefGoogle ScholarPubMed
Lia, Å, Sundberg, B Åman P, Sandberg, AS, Hallmans, G & Andersson, H (1996) Substrates available for colonic fermentation from oat, barley and wheat bread diets. Br J Nutr 76, 797–808.CrossRefGoogle ScholarPubMed
Livesey, G (1991) The energy value of carbohydrate and fibre for man. Proc Nutr Soc Aust 16, 79–88.Google Scholar
McCleary, BV & Glennie-Holmes, M (1985) Enzymatic quantification of (1–3)(1–4)-ß-D-glucan in barley and malt. J Inst Brew 91, 285–295.CrossRefGoogle Scholar
Mason, VC (1984) Metabolism of nitrogenous compounds in the large gut. Proc Nutr Soc 43, 45–53.CrossRefGoogle ScholarPubMed
Nielsen, HE (1962) Avlens og fodringens indflydelse på tarmkanalens udvikling hos svin (The influence of breeding and feeding on the development of the gastrointestinal tract of pigs). Ugeskr Landmænd 107, 235–240.Google Scholar
Nyman, M & Asp, NG (1985) Dietary fibre fermentation in the rat intestinal tract: effect of adaptation period, protein and fibre levels, and particle size. Br J Nutr 54, 635–643.CrossRefGoogle ScholarPubMed
Pearson, G, Moughan, PJ, Dong, GZ & Morel, PCH (1999) Protein quality in blood meal. I. The rat as a model animal for determining apparent ileal amino acid digestibility in the growing pig. Anim Feed Sci Technol 79, 301–307.CrossRefGoogle Scholar
Raczynski, G, Eggum, BO & Chwalibog, A (1982) The effect of dietary composition on transit time in rats. Z Tierphysiol Tierernähr Futtermittelkd 47, 160–167.CrossRefGoogle ScholarPubMed
Roth, FX & Kirchgessner, M (1984) Verdaulichkeit der Energie und Rohnährstoffe beim Schwein in Abhängigkeit von Fütterungsniveau und Lebendgewicht (Digestibility of energy and nutrients in pigs as influenced by feeding level and body weight). Z Tierphysiol Tierernähr Futtermittelkd 51, 79–87.CrossRefGoogle Scholar
Shi, XS & Noblet, J (1994) Effect of body weight and feed composition on the contribution of hindgut to digestion of energy and nutrients in pigs. Livest Prod Sci 38, 225–235.CrossRefGoogle Scholar
Smith, WC, Moughan, PJ, Pearson, G & James, KAC (1987) Comparative bioavailable energy values of five ground cereal grains measured with growing pigs and rats. Anim Feed Sci Technol 18, 143–150.CrossRefGoogle Scholar
Stanogias, G & Pearce, GR (1985) The digestion of fibre by pigs. 1. The effects of amount and type of fibre on apparent digestibility, nitrogen balance and rate of passage. Br J Nutr 53, 513–530.CrossRefGoogle ScholarPubMed
Stoldt, W (1952) Vorschlag zur Vereinheitlichung der Fettbestimmung in Lebensmitteln (Suggestions to standardize the determination of fat in foodstuffs). F Seif Anst 54, 206–207.Google Scholar
Theander, O & Westerlund, EA (1986) Studies on dietary fiber. 3. Improved procedure for analysis of dietary fiber. J Agric Food Chem 34, 330–336.CrossRefGoogle Scholar
Van Soest, P (1984) Some physical characteristics of dietary fibres and their influence on the microbial ecology of the human colon. Proc Nutr Soc 43, 25–33.CrossRefGoogle ScholarPubMed
Van Soest, PJ, Jeraci, J, Foose, T, Wrick, K & Ehle, F (1983) Comparative fermentation of fibre in man and other animals. In Fibre in Human Nutrition. pp. 75–80. [Wallace, G and Bell, L, editors]. Palmerston North: The Royal Society of New Zealand.Google Scholar
Wisker, E, Bach-Knudsen, KE, Daniel, M, Feldheim, W & Eggum, BO (1996) Digestibilities of energy, protein, fat and nonstarch polysaccharides in a low fiber diet and diets containing coarse or fine whole meal rye are comparable in rats and humans. J Nutr 126, 481–488.CrossRefGoogle ScholarPubMed
Wood, PJ (1986) Oat ß-glucan: structure, location, and properties. In Oats: Chemistry and Technology. pp. 121–152. [Webster, FH, editor]. St Paul, MN: American Association of Cereal Chemists.Google Scholar