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School-milk intervention trial enhances growth and bone mineral accretion in Chinese girls aged 10–12 years in Beijing

  • Xueqin DU (a1), Kun Zhu (a1), Angelika Trube (a1), Qian Zhang (a2), Guansheng Ma (a2), Xiaoqi Hu (a2), David R. Fraser (a1) and Heather Greenfield (a1)...


A 2-year milk intervention trial was carried out with 757 girls, aged 10 years, from nine primary schools in Beijing (April 1999 – March 2001). Schools were randomised into three groups: group 1, 238 girls consumed a carton of 330 ml milk fortified with Ca on school days over the study period; group 2, 260 girls received the same quantity of milk additionally fortified with 5 or 8 μg cholecalciferol; group 3, 259 control girls. Anthropometric and bone mineralisation measurements, as well as dietary, health and physical-activity data, were collected at baseline and after 12 and 24 months of the trial. Over the 2-year period the consumption of this milk, with or without added cholecalciferol, led to significant increases in the changes in height (≥0·6%), sitting height (≥0·8%), body weight (≥2·9%), and (size-adjusted) total-body bone mineral content (≥1·2%) and bone mineral density (≥3·2%). Those subjects receiving additional cholecalciferol compared with those receiving the milk without added 25-hydoxycholecalciferol had significantly greater increases in the change in (size-adjusted) total-body bone mineral content (2·4 v. 1·2%) and bone mineral density (5·5 v. 3·2%). The milk fortified with cholecalciferol significantly improved vitamin D status at the end of the trial compared with the milk alone or control groups. It is concluded that an increase in milk consumption, e.g. by means of school milk programmes, would improve bone growth during adolescence, particularly when Ca intake and vitamin D status are low.


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*Corresponding author: fax +61 2 9351 2114, Email


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1Adelaide Children's Hospital Stages of Puberty. Sydney. Pharmacia & Upjohn 1989.
2Bonjour, JP, Carrie, AL, Ferrari, S, Clavien, H, Slosman, D, Theintz, G & Rizzoli, RCalcium-enriched foods and bone mass growth in prepubertal girls: A randomized, double-blind, placebo-controlled trial. j Clin Invest 1997 99, 12871294.
3Cadogan, J, Eastell, R, Jones, N & Barker, MEMilk intake and bone mineral acquisition in adolescent girls: randomised, controlled intervention trial. Br Med J 1997 315, 12551260.
4Chan, GM, Hoffman, K & McMurry, MEffects of dairy products on bone and body composition in pubertal girls. J Pediatr 1995 126, 551556.
5Chinese Nutrition Society Chinese DRI's, pp. 457Beijing: Chinese Light Industry Publishing House. 2000.
6Clements, MR, Johnson, L & Fraser, DRA new mechanism for induced vitamin D deficiency in calcium deprivation. Nature 1987 325, 6265.
7Department of Health Dietary Reference Values for Food Energy and Nutrients for the United Kingdom. Report on Health and Social Subjects no. 41. London: H. M. Stationery Office. 1991.
8Dibba, B, Prentice, A, Ceesay, M, Stirling, DM, Cole, TJ & Poskitt, EMEEffect of calcium supplementation on bone mineral accretion in Gambian children accustomed to a low-calcium diet. Am J Clin Nutr 2000 71, 544549.
9Du, X, Greenfield, H, Fraser, DR, Ge, K, Liu, ZH & He, WMilk consumption and bone mineral content in Chinese adolescent girls. Bone 2002 30, 521528.
10Du, X, Greenfield, H, Fraser, DR, Ge, K, Trube, A & Wang, YVitamin D deficiency and associated factors in adolescent girls in Beijing. Am J Clin Nutr 2001 74, 494500.
11Fraser, DRNutritional growth retardation: experimental studies with special reference to calcium. In Linear Growth Retardation in Less Developed Countries, Nestlé Nutrition Workshop Series, 14, pp. 127135 [Waterlow, JC] New York: Raven Press. 1988.
12He, W, Du, X, Greenfield, HCAVD, A Survey System Using Epi Info. Beijing: Chinese Academy of Preventive Medicine (software). 1997.
13Holland, B, Welch, AA, Unwin, ID, Buss, DH, Paul, AA & Southgate, DATMcCance and Widdowson's The Composition of Foods, 5th ed. London: Royal Society of Chemistry and Ministry of Agriculture. 1991.
14Institute of Nutrition and Food Hygiene Food Composition Tables. Beijing: People's Health Publishing House. 1991.
15Johnston, CC, Miller, JZ, Slemenda, CW, Reister, TK, Hui, S, Christian, JC & Peacock, MCalcium supplementation and increases in bone mineral density in children. N Engl J Med 1992 327, 8287.
16Lee, WTK, Leung, SSF, Wang, SH, Xu, YC, Zeng, WP, Lau, J, Oppenheimer, SJ & Cheng, JCYDouble-blind, controlled calcium supplementation and bone mineral accretion in children accustomed to a low-calcium diet. Am J Clin Nutr 1994 60, 744750.
17Lloyd, T, Andon, MB, Rollings, N, Martel, JK, Landis, R, Demers, LM, Eggli, DF, Kieselhorst, K & Kulin, HECalcium supplementation and bone mineral density in adolescent girls. J Am Med Assoc 1993 270, 841844.
18Mason, RS & Posen, SSome problems associated with assay of 25-hydroxycalciferol in human serum. J Clin Chem 1977 23, 806810.
19Matkovic, V, Fontana, D, Tominac, C, Goel, P & Chesnut, CHFactors that influence peak bone mass: a study of calcium balance and the inheritance of bone mass in adolescent females. Am J Clin Nutr 1990 52, 878888.
20Matkovic, V, Jelic, T, Wardlaw, GM, Ilich, JZ, Goel, PK, Wright, JK, Andon, MB, Smith, KT & Heaney, RPTiming of peak bone mass in Caucasian females and its implication for the prevention of osteoporosis: inference from a cross-sectional model. J Clin Invest 1994 93, 799808.
21National Sports Committee Assessment of Development of Metacarpals, Phalanges and Carpals of Chinese People. Beijing: National Standard of People's Republic of China 1992.
22Tanner, JMGrowth at Adolescence, pp3036. Oxford: Blackwell Scientific Publications 1962.



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