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Serum clearance and urinary excretion of pteroylmonoglutamic acid in gestating and lactating dairy cows

  • Christiane L. Girard (a1) and J. Jacques Matte (a1)
Abstract

The present experiment was undertaken to verify if the demand of tissues for pteroylglutamic (folic) acid, evaluated by serum clearance and urinary excretion of folates, is different between multiparous dairy cows in late gestation (five gestating dry cows, 52.6 (SD 8.4) d before parturition), and in early lactation (four lactating non-gestating cows, 18.0 (SD 5.9) d after parturition). On day 1 the cows received one intravenous (i.v.) injection of 50 μg pteroylmonoglutamic acid/kg body weight (BW). Blood samples were taken at 0, 5, 15, 30 min, 1, 2, 4, 8 and 24 h after the i.v. injection. On day 3 the cows received one intramuscular (i.m.) injection of 0.3 mg pteroylmonoglutamic acid/kg BW. Blood and urine samples, as well as urine volume, were taken at 0, 1, 2, 4, 8, 12, 24, 36 and 48 h after the i.m. injection. On days 5, 6 and 7 a daily i.m. injection of 0.5 mg pteroylmonoglutamic acid/kg BW was given in an attempt to saturate tissues with folates. Then the cows received one i.v. (day 8) and one i.m. (day 10) injection of pteroylmonoglutamic acid according to the same procedures described previously for days 1 and 3. On day 12 plasma volume was determined. Before tissue saturation, serum clearance of folates during the 24 h following an i.v. injection was similar for gestating and lactating cows but after tissue saturation serum clearance was slower for lactating than gestating cows (stage x saturation, P = 0.04). The percentage of folates excreted in urine was not affected by the physiological stage (P ≥ 0.6) or the level of tissue saturation (P ≥ 0.5). In conclusion, serum clearance and urinary excretion of pteroylmonoglutamic acid seem to support the hypothesis that, in multiparous cows, although there are no deficiency symptoms, tissue demand for folic acid is high, especially during gestation.

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References
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Abramovitz, M. & Slegun, I. A. (1972). Handbook of Mathematical Functions, pp. 885887. New York: Dover Publications Inc.
Agricultural Research Council (1980). The Nutrient Requirements of Ruminant Livestock. Slough: Commonwealth Agricultural Bureaux.
Agriculture Canada (1990). Code de Pratiques pour le Soin et la Manipulation des Bovins Laitiers. Agriculture Canada PublicationOttawa:Agriculture Canada.
Bailey, L. B. (1990). Folate status assessment. Journal of Nutrition 120, 15081511.
Bhandari, S. D. & Gregory, J. F. III (1992). Folic acid, 5-methyl-tetrahydrofolate and 5-formyl-tetrahydrofolate exhibit equivalent intestinal absorption, metabolism and in vivo kinetics in rats. Journal of Nutrition 122, 18471854.
da Costa, M., Rothenberg, S. P. & Rosenberg, Z. (1979). Radiochemical method for measuring plasma clearance and urinary excretion of pteroylglutamic acid. Clinical Chemistry 25, 17831786.
Eisenga, B. H., Collins, T. D. & McMartin, K. E. (1992). Incorporation of 3H-label folic acid is tissue-dependent in folate-deficient rats. Journal of Nutrition 122, 977985.
Gill, J. L. (1978). Design and Analysis of Experiments in the Animal and Medical Sciences, Vol. 2. Ames, Iowa: The Iowa State University Press.
Girard, C. L., Chiquette, J. & Matte, J. J. (1994). Concentrations of folates in ruminal content of steers: responses to a dietary supplement of folic acid in relation with the nature of the diet. Journal of Animal Science 72, 10231028.
Girard, C. L., Matte, J. J. & Tremblay, G. F. (1989). Serum folates in gestating and lactating dairy cows. Journal of Dairy Science 72, 32403246.
Girard, C. L., Matte, J. J. & Tremblay, G. F. (1995). Gestation and lactation of dairy cows: a role for folic acid? Journal of Dairy Science 78, 404411.
Gregory, J. F. III, Bailey, L. B., Toth, J. P. & Cerda, J. J. (1990). Stable-isotope methods for assessment of folate bioavailability. American Journal of Clinical Nutrition 51, 212215.
Kownacki-Brown, P. A., Wang, C, Bailey, L. B., Toth, J. P. & Gregory, J. F. Ill (1993). Urinary excretion of deuterium-labeled folate and the metabolite p-aminobenzoylglutamate in humans. Journal of Nutrition 123, 11011108.
Landon, M. J. & Hytten, F. E. (1971). The excretion of folate in pregnancy. Journal of Obstetrics and Gynaecology of British Commonwealth 78, 769775.
McNulty, H., McPartlin, J. M., Weir, D. G. & Scott, J. M. (1993). Folate catabolism is increased during pregnancy in rats. Journal of Nutrition 123, 10891093.
Matte, J. J., Laforest, J. P., Farmer, C. & Girard, C. L. (1994). Le contrôle de la survie embryonnaire chez le pore. L'effet de l'acide folique sur certaines caractéristiques du milieu utérin et sur le développement embryonnaire. (Regulation of embryonic survival in the pig. The effect of dietary addition of folic acid to sows' diets on some aspects of uterine secretory function and embryo development.) Journées de la Recherche Porcine en France 26, 293298.
National Research Council (1988). Nutrient Requirements of Dairy Cattle, 6th ed. Washington, DC: National Academy of Sciences.
Potier, de, Courcy, G. & Terroine, T. (1979). Étude expérimentale du développement foetal dans la déflcience en acide folique par antivitamines (acide X-méthyl-folique, méthotrexate). (Fetal development under experimental folic acid deficiency induced by methotrexate or methyl-folic acid). Annates de Biologie Animate, Biochimie et Biophysique 19, 207215.
Shojania, A. M. & Hornady, G. (1970). Folate metabolism in newborns and during early infancy. II. Clearance of folic acid in plasma and excretion of folic acid in urine by newborns. Pediatric Research 4, 422426.
Snedecor, G. W. & Cochran, W. G. (1971). Méthodes Statistiques, 6th ed. Paris: Association de Coordination Technique.
Tamura, T. & Stockstad, E. L. R. (1973). The availability of food folate in man. British Journal of Haematology 25, 513532.
Tremblay, G. F., Girard, C. L., Bernier-Cardou, M. & Matte, J. J. (1991). Nycterohemeral variations of concentration of serum folates in dairy cows. Canadian Journal of Animal Science 71, 919923.
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British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
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