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Short- and long-term effects of calcium and exercise on bone mineral density in ovariectomized rats

Published online by Cambridge University Press:  09 March 2007

Joseé Gala ,
Manuel Di´az-curiel ,
Concepcioó de la Piedra  and
Jesu´s Calero
Joseé Gala
Affiliation:
Department of Internal Medicine, Fundación Jimenez Dfaz Avenida Reyes Cato´licos2, 28040 madrid, Spain
Manuel Di´az-curiel*
Affiliation:
Department of Internal Medicine, Fundación Jimenez Dfaz Avenida Reyes Cato´licos2, 28040 madrid, Spain
Concepcioó de la Piedra
Affiliation:
Biochemistry Laboratory (Bone Pathophysiology Section), Fundación Jimenez Dfaz Avenida Reyes Cato´licos2, 28040 madrid, Spain
Jesu´s Calero
Affiliation:
Biochemistry Laboratory (Bone Pathophysiology Section), Fundación Jimenez Dfaz Avenida Reyes Cato´licos2, 28040 madrid, Spain
*
*Corresponding author: Dr Díaz-Curiel, fax +34 91 5494764, email MDCuriel@fjd.es
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Abstract

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At the level of prevention of bone mineral loss produced by ovariectomy, the aim of the present study was to determine the effect produced by supplementation of Ca in the diet and a moderate exercise programme (treadmill), simultaneously or separately, in ovariectomized rats, an experimental model of postmenopausal bone loss. Female Wistar rats (n 110, 15 weeks old) were divided into five groups: (1) OVX, rats ovariectomized at 15 weeks of age, fed a standard diet; (2) SHAM, rats sham operated at 15 weeks of age, fed a standard diet; (3) OVX–EX, ovariectomized rats, fed a standard diet and performing the established exercise programme; (4) OVX–Ca, ovariectomized rats fed a diet supplemented with Ca; (5) OVX–EXCa, ovariectomized rats with the exercise programme and diet supplemented with Ca. The different treatments were initiated 1 week after ovariectomy and were continued for 13 weeks for subgroup 1 and 28 weeks for subgroup 2, to look at the interaction of age and time passed from ovariectomy on the treatments. Bone mineral density (BMD) was determined, at the end of the study, in the lumbar spine (L2, L3 and L4) and in the left femur using a densitometer. Bone turnover was also estimated at the end of the study, measuring the serum formation marker total alkaline phosphatase (AP) and the resorption marker serum tartrate-resistant acid phosphatase (TRAP). As expected, OVX rats showed a significant decrease (P<0·05) in BMD, more pronounced in subgroup 2, and a significant increase in AP and TRAP with regard to their respective SHAM group. The simultaneous treatment with Ca and exercise produced the best effects on lumbar and femoral BMD of ovariectomized rats, partially avoiding bone loss produced by ovariectomy, although it was not able to fully maintain BMD levels of intact animals. This combined treatment produced a significant increase in AP, both in subgroups 1 and 2, and a decrease in TRAP in subgroup 1, with regard to OVX group. The exercise treatment alone was able to produce an increase in BMD with regard to OVX group only in subgroup 1 of rats (younger animals and less time from ovariectomy), but not in subgroup 2. In agreement with this, there was an increase of AP in both subgroups, lower than that observed in animals submitted to exercise plus Ca supplement, and a decrease of TRAP in subgroup 1, without significant changes in this marker in the older rats. Ca treatment did not produce any significant effect on BMD in OVX rats in both subgroups of animals, showing a decrease of AP and TRAP levels in the younger animals with no significant variations in markers of bone remodelling in the older female rats compared with their respective OVX group.

Keywords

OsteoporosisCalciumExerciseBone massBone markersRat
Type
Research Article
Information
British Journal of Nutrition , Volume 86 , Issue 4 , October 2001 , pp. 521 - 527
Copyright
Copyright © The Nutrition Society 2001

References

Canalis, E (1996) Regulation of bone remodeling. In Primer on the Metabolic Bone Diseases and Disorders of Mineral Metabolism, 3rd ed, pp. 29–34 [Favus, M, editor]. Philadelphia, New York: Lippincott-Raven.Google Scholar
Chen, MM, Yeh, JK, Aloia, JF, Tierney, JM & Sprintz, S (1994) Effect of treadmill exercise on tibial cortical bone in aged female rats: A histomorphometry and dual energy X-ray absorptiometry study. Bone 15, 313–319.Google Scholar
Cheng, S, Suominen, H, Rantanen, T, Parkatti, T & Heikkinen, E (1991) Bone mineral density and physical activity in 50–60-year-old women. Bone and Mineral 12, 123–132.CrossRefGoogle ScholarPubMed
Damien, E, Price, JS & Lanyon, LE (1998) The estrogen receptor's involvement in osteoblasts' adaptive response to mechanical strain. Journal of Bone and Mineral Research 13, 1275–1282.Google Scholar
Dawson-Hughes, B, Dallal, GE, Krall, EA, Sadowski, L, Sahyoun, N & Tannenbaum, S (1991) A controlled trial of the effect of calcium supplementation on bone density in postmenopausal women. New England Journal of Medicine 323, 878–883.CrossRefGoogle Scholar
Dempster, DW & Lindsay, R (1993) Pathogenesis of osteoporosis. Lancet 341, 797–801.Google Scholar
Díaz-Curiel, M, Calero, JA, Guerrero, R, Gala, JL, Gazapo, R & de la Piedra, C (1998) Effects of LY 117018 HCl on bone remodeling and mineral density in the oophorectomized rat. American Journal of Obstetrics and Gynecology 178, 320–325.CrossRefGoogle Scholar
Díaz-Curiel, M & Gala, JL (1994) $$$Es la rata ooforectomizada un modelo apropiado para el estudio de la osteoporosis postmenopaúsica humana? (Is the ovariectomized rat an appropriate model to study osteoporosis in postmenopausal human-subjects?). Revista Española de Enfermedades Metabólicas Óseas 3, 83–84.Google Scholar
Frost, HM & Jee, WSS (1992) On the rat model of human osteopenias and osteoporoses. Bone and Mineral 18, 227–236.CrossRefGoogle ScholarPubMed
Fujita, T, Fujii, Y, Kitagawa, R & Fukase, M (1993) Calcium supplementation in osteoporosis. Osteoporosis International 3 Suppl. 1, 159–162.Google Scholar
Gala, J, Díaz-Curiel, M, de la Piedra, C, Castilla, C & Torralbo, M (1998) Bone mass assessment in rats by dual energy X-ray absorptiometry. British Journal of Radiology 71, 754–758.CrossRefGoogle Scholar
Garnero, P, Sornay-Rendu, E, Chapuy, MC & Delmas, PD (1996) Increased bone turnover in late postmenopausal women is a major determinant of osteoporosis. Journal of Bone and Mineral Research 11, 337–349.Google Scholar
Heaney, RP (1989) The calcium controversy: finding a middle ground between the extremes. Public Health Reports 104 Suppl., 36–46.Google ScholarPubMed
Heaney, RP (1991) Effect of calcium on skeletal development, bone loss, and risk of fractures. American Journal of Medicine 91, 23S–28S.Google Scholar
Iwamoto, J, Takeda, T & Ichimura, S (1998 a) Effects of exercise on bone mineral density in mature osteopenic rats. Journal of Bone and Mineral Research 13, 1308–1317.Google Scholar
Iwamoto, J, Takeda, T & Ichimura, S (1998 b) Effect of exercise on tibial and lumbar vertebral bone mass in mature osteopenic rats: bone histomorphometry study. Journal of Orthopaedic Science 3, 257–263.Google ScholarPubMed
Kalu, DN (1991) Review: The ovariectomized rat model of postmenopausal bone loss. Bone and Mineral 15, 175–192.CrossRefGoogle ScholarPubMed
Kardinaal, AFM, Ando, S, Charles, P, Charzewska, J, Rotily, M, Väänänen, K, Van Erp-Baart, AMJ, Heikkinen, J, Thomsen, J, Maggiolini, M, Deloraine, A, Chabros, E, Juvin, R & Schaafsma, G (1999) Dietary calcium and bone density in adolescent girls and young women in Europe. Journal of Bone and Mineral Research 14, 583–592.Google Scholar
Kippo, K, Hannuniemi, R, Isaksson, P, Lauren, L, Österman T, Peng, Z, Tuukkanen, J, Kuurtamo, P, Väänanen, HK & Sellman, R (1998) Clodronate prevents osteopenia and loss of trabecular connectivity in estrogen-deficient rats. Journal of Bone and Mineral Research 13, 287–296.CrossRefGoogle ScholarPubMed
Lau, EM, Woo, J, Leung, PC, Swamminathan, R & Leung, D (1992) The effects of calcium supplementation and exercise on bone density in elderly Chinese women. Osteoporosis International 2, 168–173.CrossRefGoogle Scholar
Ly, CY, Chuda, RA & Lam, WKW (1973) Acid phosphatases in human plasma. Journal of Laboratory and Clinical Medicine 82, 446–460.Google Scholar
Mazess, R & Barden, H (1991) Bone density in premenopausal women: effects of age, dietary intake, physical activity, smoking and birth-control pills. American Journal of Clinical Nutrition 53, 132–142.Google Scholar
Minaire, P (1989) Immobilization osteoporosis: a review. Clinical Rheumatology 8, 95–103.Google Scholar
Mosekilde, L, Danielsen, CC, Sogaard, CH & Thorling, E (1994) The effect of long-term exercise on vertebral and femoral bone mass, dimensions, and strength. Assessed in a rat model. Bone 15, 293–301.Google Scholar
Moss, DW (1982) Alkaline phosphatase isoenzymes (Review). Clinical Chemistry 28, 2007–2016.CrossRefGoogle Scholar
Newhall, KM, Rodnick, KJ, van der Meulen, MC, Carter, DR & Marcus, R (1991) Effects of voluntary exercise on bone mineral content in rats. Journal of Bone and Mineral Research 6, 289–296.Google Scholar
Nguyen, TV, Center, JR & Eisman, JA (2000) Osteoporosis in elderly men and women: Effects of dietary calcium, physical activity and body mass index. Journal of Bone and Mineral Research 15, 322–331.Google Scholar
Nordin, BEC & Morris, HA (1989) The calcium deficiency model for osteoporosis. Nutrition Reviews 47, 65–72.CrossRefGoogle ScholarPubMed
Nordin, BE, Need, AG, Morris, HA, Horowitz, M & Robertson, WG (1991) Evidence for a renal calcium leak in postmenopausal women. Journal of Clinical Endocrinology and Metabolism 72, 401–407.CrossRefGoogle ScholarPubMed
Peterson, CA, Eurell, JA & Erdman, JW (1995) Alterations in calcium intake on peak bone mass in the female rat. Journal of Bone and Mineral Research 10, 81–95.Google Scholar
Raab, DM, Smith, EL, Crenshaw, TD & Thomas, DP (1990) Bone mechanical properties after exercise training in young and old rats. Journal of Applied Physiology 68, 130–134.Google Scholar
Riis, B, Thomsen, K & Christiansen, C (1987) Does calcium supplementation prevent postmenopausal bone loss? A double-blind study. New England Journal of Medicine 316, 173–177.CrossRefGoogle Scholar
Sato, M, McClintock, C, Kim, J, Turner, CH, Bryant, HU, Magee, D & Slemenda, CW (1994) Dual-energy X-ray absorptiometry of raloxifene effects on the lumbar vertebrae and femora of ovariectomized rats. Journal of Bone and Mineral Research 9, 715–724.CrossRefGoogle ScholarPubMed
Smith, EL & Raab, DM (1986) Osteoporosis and physical activity. Acta Medica Scandinavica 711, 149–156.CrossRefGoogle ScholarPubMed
Specker, BL (1996) Evidence for an interaction between calcium intake and physical activity on changes in bone mineral density. Journal of Bone and Mineral Research 11, 1539–1544.Google Scholar
Spelsberg, TM, Subramaniam, M, Riggs, L & Koshla, S (1999) The actions and interactions of sex steroids and growth factors/cytokines on the skeleton. Molecular Endocrinology 13, 819–828.Google Scholar
Wang, CH & Zhang, Y (1998) Bone mineral density of adult female rats subjected to dietary energy restriction and running exercise. Bone 23, S237.Google Scholar
Wronsky, TJ, Yen, CF, Burton, KW, Mehta, RC & Newman, PS (1991) Skeletal effects of calcitonin in ovariectomized rats. Endocrinology 129, 2246–2250.Google Scholar
Yeh, JK, Aloia, JF, Chen, MM, Tierney, JM & Sprintz, S (1993 a) Influence of exercise on cancellous bone of the aged female rat. Journal of Bone and Mineral Research 8, 1117–1125.CrossRefGoogle ScholarPubMed
Yeh, JK, Aloia, JF, Chen, MM, Tierney, JM & Sprintz, S (1994) Effect of treadmill exercise on vertebral and tibial bone mineral content and bone mineral density in the aged adult rat: Determined by dual energy x-ray absorptiometry. Calcified Tissue International 52, 234–238.CrossRefGoogle Scholar
Yeh, JK, Liu, CC & Aloia, JF (1993 b) Effects of exercise and immobilization on bone formation and resorption in young rats. American Journal of Physiology 264, E182–E189.Google Scholar
Zaidi, M, Moonga, B, Moss, DW & McIntyre, I (1989) Inhibition of osteoclastic acid phosphatase abolishes bone resorption. Biochimica Biophysica Research Communications 159, 68–71.CrossRefGoogle ScholarPubMed
Zaman, G, Suswillo, RFL, Cheng, MZ, Tavares, IA & Lanyon, LE (1997) Early responses to dynamic strain change and prostaglandins in bone-derived cells in culture. Journal of Bone and Mineral Research 12, 769–777.Google Scholar