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Supplemental zinc reduced intestinal permeability by enhancing occludin and zonula occludens protein-1 (ZO-1) expression in weaning piglets

  • Bingkun Zhang (a1) and Yuming Guo (a1)
Abstract

The present study was carried out to evaluate the pharmacological effect of Zn in diarrhoea in relation to intestinal permeability. Seventy-two weaning piglets, aged 24 d, were allocated to three dietary treatments: (1) control diet without supplemental Zn; (2) control diet supplemented with 2000 mg Zn/kg from ZnO; (3) control diet supplemented with 2000 mg Zn/kg from tetrabasic zinc chloride (TBZC). At the end of a 14 d experiment period, piglets were weighed, feed consumption was measured, and mucosal barrier function was determined using the lactulose/mannitol test. Expression of mucosal tight junction protein was measured at RNA and protein level. Inclusion of TBZC or ZnO in the diet significantly increased average daily gain (P < 0·01) and average daily feed intake (P < 0·05), while leading to reduced feed conversion ratio (P < 0·05) and faecal scores (P < 0·01). TBZC reduced urinary lactulose:mannitol ratios of weaning piglets (P < 0·05), while dietary supplementation with ZnO tended to reduce urinary lactulose:mannitol ratios (P = 0·061). ZnO or TBZC significantly enhanced the mRNA and protein expression of occludin (P < 0·05) and zonula occludens protein-1 (ZO-1) (P < 0·05) in the ileal mucosa. Piglets fed the TBZC-supplemented diet had a higher level of occludin than pigs fed the ZnO-supplemented diet (P < 0·05). The results indicate that Zn supplementation decreased faecal scores and the reduction was accompanied by reduced intestinal permeability, which was evident from the reduced urinary lactulose:mannitol ratios and increased expression of occludin and ZO-1. Therefore, the protective effect of pharmacological levels of dietary Zn in reducing diarrhoea might, at least partly, be associated with reduced intestinal permeability.

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      Supplemental zinc reduced intestinal permeability by enhancing occludin and zonula occludens protein-1 (ZO-1) expression in weaning piglets
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Corresponding author
*Corresponding author: Professor Yuming Guo, fax +86 10 62733900, email guoyum@cau.edu.cn
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1 JR Pluske , DJ Hampson & IH Williams (1997) Factors influencing the structure and function of the small intestine in the weaned pig: a review. Livest Prod Sci 51, 215236.

4 BK Zhang & YM Guo (2007) Beneficial effects of tetrabasic zinc chloride for weanling piglets and the bioavailability of zinc in tetrabasic form relative to ZnO. Anim Feed Sci Tech 135, 7585.

5 BK Zhang & YM Guo (2007) The growth-promoting effect of tetrabasic zinc chloride is associated with elevated concentration of growth hormone and ghrelin. Asian Aust J Anim Sci 21, 14731478.

7 RPK Ford , IS Menzies , AD Phillips , (1985) Intestinal sugar permeability: relationship to diarrhoeal disease and small bowel morphology. J Pediatr Gastr Nutr 4, 568574.

8 E Smecuol , JC Bai , H Vazquez , (1997) Gastrointestinal permeability in celiac disease. Gastroenterology 112, 11291136.

9 GC Sturniolo , W Fries , E Mazzon , (2002) Effect of zinc supplementation on intestinal permeability in experimental colitis. J Lab Clin Med 139, 311315.

10 SK Roy , RH Behrens , R Haider , (1992) Impact of zinc supplementation on intestinal permeability in Bangladeshi children with acute diarrhea and persistent diarrhea syndrome. J Pediatr Gastr Nutr 15, 289296.

11 S Sazawal , RE Black , MK Bhan , (1995) Zinc supplementation in young children with acute diarrhea in India. N Engl J Med 333, 839844.

12 P Rodríguez , N Darmon , P Chappuis , (1996) Intestinal paracellular permeability during malnutrition in guinea pigs: effect of high dietary zinc. Gut 39, 416422.

13 ST Ballard , JH Hunter & AE Taylor (1995) Regulation of tight-junction permeability during nutrient absorption across the intestinal epithelium. Annu Rev Nutr 15, 3555.

14 AT Blikslager , AJ Moeser , JL Gookin , (2007) Restoration of barrier function in injured intestinal mucosa. Physiol Rev 87, 545564.

16 M Generoso , DR Mario , DR Roberto , (2003) Cellobiose and lactulose coupled with mannitol and determined using ion-exchange chromatography with pulsed amperometric detection, are reliable probes for investigation of intestinal permeability. J Chromatogr B 783, 349357.

17 I Bjarnason , A MacPherson & D Hollander (1995) Intestinal permeability: an overview. Gastroenterology 108, 15661581.

19 I Mavromichalis , DM Webel , EN Parr , (2001) Growth-promoting efficacy of pharmacological doses of tetrabasic zinc chloride in diets for nursery pigs. Can J Anim Sci 81, 387391.

21 JMS Barboza , TMJ Silva , RL Guerrent , (1999) Measurement of intestinal permeability using mannitol and lactulose in children with diarrheal diseases. Braz J Med Biol Res 32, 14991504.

22 M Elia , R Behrens , C Northrop , (1987) Evaluation of mannitol, lactulose and 51Cr-labelled ethylenediaminetetra-acetate as markers of intestinal permeability in man. Clin Sci 73, 197204.

23 LT Weaver , MF Laker , R Nelson , (1987) Milk feeding and changes in intestinal permeability and morphology in the newborn. J Pediatr Gastr Nutr 6, 351358.

26 BM Gumbiner (1993) Breaking through the tight junction barrier. J Cell Biol 123, 16311633.

27 T Kucharzik , SV Walsh , J Chen , (2001) Neutrophil transmigration in inflammatory bowel disease is associated with differential expression of epithelial intercellular junction proteins. Am J Pathol 159, 20012009.

29 V Wong & BM Gumbiner (1997) A synthetic peptide corresponding to the extracellular domain of occludin perturbs the tight junction permeability barrier. J Cell Biol 136, 399409.

31 M Furuse , K Fujita , T Hiiragi , (1998) Claudin-1 and claudin-2, novel integral membrane proteins localizing at tight junctions with no sequence similarity to occludin. J Cell Biol 141, 15391550.

32 K Morita , K Furuse , K Fujimoto , (1999) Claudin multigene family encoding four-transmembrane domain protein components of tight junction strands. Proc Natl Acad Sci U S A 96, 511516.

33 BR Stevenson , JD Silicano , M Mooseker , (1986) Identification of ZO-1: a high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia. J Cell Biol 103, 755766.

34 AS Fanning , BJ Jameson , LA Jesaitis , (1998) The tight junction protein ZO-1 establishes a link between the transmembrane protein occludin and the actin cytoskeleton. J Biol Chem 273, 2974529753.

35 M Furuse , T Hirase , M Itoh , (1996) Occludin: a novel integral membrane protein localizing at tight junctions. J Cell Biol 123, 17771788.

37 J Holmes , C Van Itallie , J Rasmussen , (2006) Claudin profiling in the mouse during postnatal intestinal development and along the gastrointestinal tract reveals complex expression patterns. Gene Expr Patterns 6, 581588.

38 J Osek (1999) Prevalence of virulence factors of Escherichia coli strains isolated from diarrheic and healthy piglets after weaning. Vet Microbiol 68, 209217.

39 M Bruewer , A Luegering , T Kucharzik , (2003) Proinflammatory cytokines disrupt epithelial barrier function by apoptosis-independent mechanisms. J Immunol 171, 61646172.

41 A Farhadi , A Banan , J Fields , (2003) Intestinal barrier: an interface between health and disease. J Gastroenterol Hepatol 18, 479497.

42 MS Balda , JA Whitney , C Flores , (1996) Functional dissociation of paracellular permeability and transepithelial electrical resistance and disruption of the apical–basolateral intramembrane diffusion barrier by expression of a mutant tight junction membrane protein. J Cell Biol 134, 10311049.

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British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
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