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Supplementation of diets with bovine colostrum influences immune function in dogs

  • Ebenezer Satyaraj (a1), Arleigh Reynolds (a1), Robyn Pelker (a1), Jeff Labuda (a1), Peifang Zhang (a1) and Peichuan Sun (a1)...


While the need for colostrum in neonates is well established, the systemic effect of feeding bovine colostrum (BC) to adult humans is gaining increasing attention. However, no systematic studies evaluating the immunomodulatory effect of BC in dogs have been reported. The aim of the present study was to evaluate the immunomodulatory effect of dietary supplementation of BC in dogs. The study was conducted in two phases: pre-test (8 weeks) and test (40 weeks), with twenty-four dogs (mean age 2·5 years) randomised into two groups. In the ‘pre-test’ phase, both groups were fed a nutritionally complete diet. At the end of the ‘pre-test’ phase, all dogs received a canine distemper virus (CDV) vaccine, and dogs in the ‘test group’ were switched to a diet supplemented with 0·1 % spray-dried BC. Response to the CDV vaccine was evaluated by measuring vaccine-specific plasma IgG levels. Gut-associated lymphoid tissue response was assessed by measuring faecal IgA levels. Gut microbiota were evaluated by the temporal temperature gel electrophoresis methodology. Dogs fed the BC-supplemented diet demonstrated a significantly higher vaccine response and higher levels of faecal IgA when compared with the control group. Supplementing diets with BC also resulted in significantly increased gut microbiota diversity and stability in the test group. In conclusion, diets supplemented with BC significantly influence immune response in dogs.

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Corresponding author

*Corresponding author: Dr E. Satyaraj, fax +1 314 982 5857, email


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1Kelly, D & Coutts, AGP (2000) Early nutrition and the development of immune function in the neonate. Proc Nutr Soc 59, 177185.
2Uruakpa, FO, Ismond, MAH & Akobundu, ENT (2002) Colostrum and its benefits: a review. Nutr Res 22, 755767.
3Alexieva, B, Markova, T & Nikolova, E (2004) Bovine colostrum – the promising nutraceutical. Czech J Food Sci 22, 7379.
4Vacher, PY & Blum, JW (1993) Age dependency of insulin like growth factor 1, insulin protein and immunoglobulin concentrations and gamma glutamyl transferase activity in first colostrum of dairy cows. Milchwissenschaft 48, 423425.
5Hagiwara, K, Kataoka, S, Yamanaka, H, et al. (2000) Detection of cytokines in bovine colostrum. Vet Immunol Immunopathol 76, 183190.
6Playford, RJ, MacDonald, CE & Johnson, WS (2000) Colostrum and milk-derived peptide growth factors for the treatment of gastrointestinal disorders. Am J Clin Nutr 72, 514.
7Shing, CM, Peake, J, Suzuki, K, et al. (2007) Effects of bovine colostrum supplementation on immune variables in highly trained cyclists. J Appl Physiol 102, 11131122.
8Mero, A, Kahkonen, J, Nykanen, T, et al. (2002) IGF-I, IgA, and IgG responses to bovine colostrum supplementation during training. J Appl Physiol 93, 732739.
9Mietens, C, Keinhorst, H, Hilpert, H, et al. (1979) Treatment of infantile E. coli gastroenteritis with specific bovine anti-E. coli milk immunoglobulins. Eur J Ped 132, 239252.
10Tacket, CO, Losonsky, G, Link, H, et al. (1988) Protection by milk immunoglobulin concentrate against oral challenge with enterogenic Escherichia coli. Eng J Med 12401241.
11Tzipori, CO, Binion, SB, Bostwick, E, et al. (1986) Remission of diarrhea due to cryptosporidiosis in an immunodeficient child treated with hyperimmune bovine colostrum. Br Med J 293, 12761277.
12Mehra, R, Marnila, P & Korhonen, H (2006) Milk immunoglobulins for health promotion. Int Dairy J 16, 12621271.
13Brussow, H, Hilpert, H, Walther, J, et al. (1987) Bovine milk immunoglobulins for passive immunity to infantile rotavirus gastroenteritus. J Clin Microbiol 25, 982986.
14Ebina, T, Sato, A, Umezu, K, et al. (1985) Prevention of rotavirus infection by oral administration of cow colostrum containing antihuman rotavirus antibody. Med Microbiol Immunol 174, 177185.
15Hilpert, H, Brussow, H, Meitens, C, et al. (1987) Use of bovine milk concentrate containing antibody to rotavirus to treat rotavirus gastroenteritis in infants. J Infect Dis 156, 158166.
16Tacket, CO, Binion, SB, Bostwick, E, et al. (1992) Efficacy of bovine milk immunoglobulin concentrate in preventing illness after Shigella flexneri challenge. Am J Trop Med Hyg 47, 276283.
17Romeo, J, Jimenez-Pavon, D, Cervantes-Borunda, M, et al. (2008) Immunological changes after a single bout of moderate-intensity exercise in a hot environment. J Physiol Biochem 64, 197204.
18Shing, CM, Peake, JM, Ahern, SM, et al. (2007) The effect of consecutive days of exercise on markers of oxidative stress. Appl Physiol Nutr Metab 32, 677685.
19Tsai, YL & Olson, BH (1992) Rapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reaction. Appl Environ Microbiol 58, 22922295.
20Deplancke, B, Hristova, KR, Oakley, HA, et al. (2000) Molecular ecological analysis of the succession and diversity of sulfate-reducing bacteria in the mouse gastrointestinal tract. Appl Environ Microbiol 66, 21662174.
21McCracken, VJ, Simpson, JM, Mackie, RI, et al. (2001) Molecular ecological analysis of dietary and antibiotic-induced alterations of the mouse intestinal microbiota. J Nutr 131, 18621870.
22Otabe, K, Ito, T, Sugimoto, T, et al. (2000) C-reactive protein (CRP) measurement in canine serum following experimentally-induced acute gastric mucosal injury. Lab Anim 34, 434438.
23Giffard, CJ, Seino, MM, Markwell, PJ, et al. (2004) Benefits of bovine colostrum on fecal quality in recently weaned puppies. J Nutr 134, 2126S2127S.
24Kraehenbuhl, JP & Neutra, MR (1992) Molecular and cellular basis of immune protection of mucosal surfaces. Physiol Rev 72, 853879.
25Holmgren, J, Czerkinsky, C, Lycke, N, et al. (1992) Mucosal immunity: implications for vaccine development. Immunobiology 184, 157179.
26McGhee, JR, Mestecky, J, Dertzbaugh, MT, et al. (1992) The mucosal immune system: from fundamental concepts to vaccine development. Vaccine 10, 7588.
27Ho, CK & Babiuk, LA (1979) Immune mechanisms against canine distemper. II. Role of antibody in antigen modulation and prevention of intercellular and extracellular spread of canine distemper virus. Immunology 38, 765772.
28Burns, EA, Lum, LG, Seigneuret, MC, et al. (1990) Decreased specific antibody synthesis in old adults: decreased potency of antigen-specific B cells with aging. Mech Ageing Dev 53, 229241.
29Patriarca, PA (1994) A randomized controlled trial of influenza vaccine in the elderly. Scientific scrutiny and ethical responsibility. JAMA 272, 17001701.
30Marnell, L, Mold, C & Du Clos, TW (2005) C-reactive protein: ligands, receptors and role in inflammation. Clin Immunol 117, 104111.
31Golbasi, Z, Ucar, O, Keles, T, et al. (2002) Increased levels of high sensitive C-reactive protein in patients with chronic rheumatic valve disease: evidence of ongoing inflammation. Eur J Heart Fail 4, 593595.
32Otabe, K, Sugimoto, T, Jinbo, T, et al. (1998) Physiological levels of C-reactive protein in normal canine sera. Vet Res Commun 22, 7785.
33Nakamura, M, Takahashi, M, Ohno, K, et al. (2008) C-reactive protein concentration in dogs with various diseases. J Vet Med Sci 70, 127131.
34Mai, V, Braden, CR, Heckendorf, J, et al. (2006) Monitoring of stool microbiota in subjects with diarrhea indicates distortions in composition. J Clin Microbiol 44, 45504552.
35Kuehl, CJ, Wood, HD, Marsh, TL, et al. (2005) Colonization of the cecal mucosa by Helicobacter hepaticus impacts the diversity of the indigenous microbiota. Infect Immun 73, 69526961.
36Satyaraj, E (2011) Emerging paradigms in immunonutrition. Top Companion Anim Med 26, 2532.
37Das, UN (2010) Obesity: genes, brain, gut, and environment. Nutrition 26, 459473.


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