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Trans-fatty acids induce pro-inflammatory responses and endothelial cell dysfunction

  • Kevin A. Harvey (a1), Tyler Arnold (a1), Tamkeen Rasool (a1), Caryl Antalis (a1), Steven J. Miller (a2) and Rafat A. Siddiqui (a1) (a3) (a4)...
Abstract

Epidemiological data indicate that there is a strong association between intake of trans-18 : 2 fatty acids (TFA) and sudden cardiac death. There is little known about the mechanisms by which TFA exert harmful effects on the cardiovascular system. The present in vitro study is the first to demonstrate the effects of membrane-incorporated C18 : 2 TFA on human aortic endothelial cell (HAEC) function. Trans-18 : 2 fatty acids were incorporated to a greater extent (2-fold) in the phospholipid fraction of endothelial cells than that of cis-18 : 2; furthermore, these fatty acids were enriched to a similar extent in the TAG fraction. Flow cytometric analysis indicated that TFA treatment of HAEC significantly increased the expression of endothelial adhesion molecules, including intercellular adhesion molecule-1 (CD54) and vitronectin receptor (CD51/CD61). Incorporation of TFA into membranes increased HAEC adhesion to fibronectin- or vitronectin-coated plates by 1·5- to 2-fold, respectively. Neutrophil and monocyte adhesion to HAEC monolayers was nearly proportional to adhesion molecule expression. TFA treatment also induced the release of monocyte chemoattractant protein-1 by nearly 3-fold in non-stimulated HAEC. Furthermore, we examined the role of TFA on in vitro angiogenic assays. Chemotactic migration of TFA-treated HAEC toward sphingosine-1-phosphate (SPP) was significantly increased compared with controls. Conversely, capillary morphogenesis of TFA-treated HAEC was significantly inhibited in response to SPP, suggesting that TFA incorporation suppresses endothelial cell differentiation. In conclusion, these in vitro studies demonstrated that TFA play a role in the induction of pro-inflammatory responses and endothelial cell dysfunction.

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Corresponding author
*Corresponding author: Dr Rafat Siddiqui, fax +1 317 962 9369, email rsiddiqu@clarian.org
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1 EA Emken (1984) Nutrition and biochemistry of trans and positional fatty acid isomers in hydrogenated oils. Annu Rev Nutr 4, 339376.

4 AH Lichtenstein (2000) Dietary trans fatty acid. J Cardiopulm Rehabil 20, 143146.

5 Z Kemeny , K Recseg , G Henon , K Kovari & F Zwobada (2001) Deodorization of vegetable oils: prediction of trans polyunsaturated fatty acid content. J Am Oil Chem Soc 78, 973979.

6 WC Willett (2006) Trans fatty acids and coronary heart disease – epidemiological data. Atherosclerosis 7, Suppl., 58.

7 D Kromhout , A Menotti , B Bloemberg , (1995) Dietary saturated and trans fatty acids and cholesterol and 25-year mortality from coronary heart disease: the Seven Countries Study. Prev Med 24, 308315.

8 A Ascherio , EB Rimm , EL Giovannucci , D Spiegelman , M Stampfer & WC Willett (1996) Dietary fat and risk of coronary heart disease in men: cohort follow up study in the United States. BMJ 313, 8490.

9 P Pietinen , A Ascherio , P Korhonen , AM Hartman , WC Willett , D Albanes & J Virtamo (1997) Intake of fatty acids and risk of coronary heart disease in a cohort of Finnish men. The Alpha-Tocopherol, Beta-Carotene Cancer Prevention Study. Am J Epidemiol 145, 876887.

10 FB Hu , MJ Stampfer , JE Manson , E Rimm , GA Colditz , BA Rosner , CH Hennekens & WC Willet (1997) Dietary fat intake and the risk of coronary heart disease in women. N Engl J Med 337, 14911499.

11 S Stender , J Dyerberg , G Holmer , L Ovesen & B Sandstrom (1995) The influence of trans fatty acids on health: a report from the Danish Nutrition Council. Clin Sci (Colch) 88, 375392.

12 MJ Stampfer , FM Sacks , S Salvini , WC Willett & CH Hennekens (1991) A prospective study of cholesterol, apolipoproteins, and the risk of myocardial infarction. N Engl J Med 325, 373381.

13 A Ascherio , MB Katan , PL Zock , MJ Stampfer & WC Willett (1999) Trans fatty acids and coronary heart disease. N Engl J Med 340, 19941998.

14 MB Katan (2006) Regulation of trans fats: the gap, the Polder, and McDonald's French fries. Atheroscler Suppl 7, 6366.

16 A Ascherio (2002) Epidemiologic studies on dietary fats and coronary heart disease. Am J Med 113, Suppl. 9B, 9S12S.

18 FA Kummerow , Q Zhou , MM Mahfouz , MR Smiricky , CM Grieshop & DJ Schaeffer (2004) Trans fatty acids in hydrogenated fat inhibited the synthesis of the polyunsaturated fatty acids in the phospholipid of arterial cells. Life Sci 74, 27072723.

21 NM de Roos , ML Bots & MB Katan (2001) Replacement of dietary saturated fatty acids by trans fatty acids lowers serum HDL cholesterol and impairs endothelial function in healthy men and women. Arterioscler Thromb Vasc Biol 21, 12331237.

24 D English , M Martin , KA Harvey , LP Akard , R Allen , TS Widlanski , JG Garcia & RA Siddiqui (1997) Characterization and purification of neutrophil ecto-phosphatidic acid phosphohydrolase. Biochem J 324, 941950.

25 MC de Almeida , AC Silva , A Barral & M Barral (2000) A simple method for human peripheral blood monocyte isolation. Mem Inst Oswaldo Cruz 95, 221223.

26 D English , AT Kovala , Z Welch , KA Harvey , RA Siddiqui , DN Brindley & JG Garcia (1999) Induction of endothelial cell chemotaxis by sphingosine 1-phosphate and stabilization of endothelial monolayer barrier function by lysophosphatidic acid, potential mediators of hematopoietic angiogenesis. J Hematother Stem Cell Res 8, 627634.

28 K Harvey , Z Welch , AT Kovala , JG Garcia & D English (2002) Comparative analysis of in vitro angiogenic activities of endothelial cells of heterogeneous origin. Microvasc Res 63, 316326.

29 K Harvey , RA Siddiqui , D Sliva , JG Garcia & D English (2002) Serum factors involved in human microvascular endothelial cell morphogenesis. J Lab Clin Med 140, 188198.

30 RN Lemaitre , IB King , D Mozaffarian , N Sotoodehnia , TD Rea , LH Kuller , RP Tracy & DS Siscovick (2006) Plasma phospholipid trans fatty acids, fatal ischemic heart disease, and sudden cardiac death in older adults: the cardiovascular health study. Circulation 114, 209215.

31 M Lagarde , B Sicard , M Guichardant , O Felisi & M Dechavanne (1984) Fatty acid composition in native and cultured human endothelial cells. In Vitro 20, 3337.

33 E Stachowska , B Dolegowska , D Chlubek , T Wesolowska , K Ciechanowski , P Gutowski , H Szumiłowicz & R Turowski (2004) Dietary trans fatty acids and composition of human atheromatous plaques. Eur J Nutr 43, 313318.

34 Q Sun , J Ma , H Campos , SE Hankinson , JE Manson , MJ Stampfer , KM Rexrode , WC Willett & FBA Hu (2007) A prospective study of trans fatty acids in erythrocytes and risk of coronary heart disease. Circulation 115, 18581865.

35 RN Lemaitre , IB King , TE Raghunathan , RM Pearce , S Weinmann , RH Knopp , MK Copass , LA Cobb & DS Siscovick (2002) Cell membrane trans-fatty acids and the risk of primary cardiac arrest. Circulation 105, 697701.

36 JW Bortolotto , C Reis , A Ferreira , S Costa , CC Mottin , AA Souto & RM Guaragan (2005) Higher content of trans fatty acids in abdominal visceral fat of morbidly obese individuals undergoing bariatric surgery compared to non-obese subjects. Obes Surg 15, 12651270.

37 HM Vidgren , AM Louheranta , JJ Agren , US Schwab & MI Uusitupa (1998) Divergent incorporation of dietary trans fatty acids in different serum lipid fractions. Lipids 33, 955962.

39 S-L Niu , DC Mitchell & BJ Litman (2005) Trans fatty acid derived phospholipids show increased membrane cholesterol and reduced receptor activation as compared to their cis analogs. Biochemistry 44, 44584465.

40 DA Brown (2006) Lipid rafts, detergent-resistant membranes, and raft targeting signals. Physiology 21, 430439.

41 M Takeya , T Yoshimura , EJ Leonard & K Takahashi (1993) Detection of monocyte chemoattractant protein-1 in human atherosclerotic lesions by an anti-monocyte chemoattractant protein-1 monoclonal antibody. Hum Pathol 24, 534539.

42 D English , Z Welch , AT Kovala , K Harvey , OV Volpert , DN Brindley & JG Garcia (2000) Sphingosine 1-phosphate released from platelets during clotting accounts for the potent endothelial cell chemotactic activity of blood serum and provides a novel link between hemostasis and angiogenesis. FASEB J 14, 22552265.

43 JG Garcia , F Liu , AD Verin , A Birukova , MA Dechert , WT Gerthoffer , JR Bamberg & D English (2001) Sphingosine 1-phosphate promotes endothelial cell barrier integrity by Edg-dependent cytoskeletal rearrangement. J Clin Invest 108, 689701.

44 Z Mallat & A Tedgui (2000) Apoptosis in the vasculature: mechanisms and functional importance. Br J Pharmacol 130, 947962.

47 D Mozaffarian (2006) Trans fatty acids – effects on systemic inflammation and endothelial function. Atheroscler Suppl 7, 2932.

48 D Kaul (2001) Molecular link between cholesterol, cytokines and atherosclerosis. Mol Cell Biochem 219, 6571.

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British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
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