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Genomic structure, organization and localization of the acetylcholinesterase locus of the olive fruit fly, Bactrocera oleae

Published online by Cambridge University Press:  24 September 2012

E.G. Kakani
Affiliation:
Department of Biochemistry and Biotechnology, University of Thessaly, Greece
M. Trakala
Affiliation:
Department of Biochemistry and Biotechnology, University of Thessaly, Greece
E. Drosopoulou
Affiliation:
Department of Biology, Aristotle University of Thessaloniki, Greece
P. Mavragani-Tsipidou
Affiliation:
Department of Biology, Aristotle University of Thessaloniki, Greece
K.D. Mathiopoulos*
Affiliation:
Department of Biochemistry and Biotechnology, University of Thessaly, Greece
*
*Author for correspondence Fax: 2410-565290 E-mail: kmathiop@bio.uth.gr

Abstract

Acetylcholinesterase (AChE), encoded by the ace gene, is a key enzyme of cholinergic neurotransmission. Insensitive acetylcholinesterase (AChE) has been shown to be responsible for resistance to OPs and CBs in a number of arthropod species, including the most important pest of olives trees, the olive fruit fly Bactrocera oleae. In this paper, the organization of the B. oleae ace locus, as well as the structural and functional features of the enzyme, are determined. The organization of the gene was deduced by comparison to the ace cDNA sequence of B. oleae and the organization of the locus in Drosophila melanogaster. A similar structure between insect ace gene has been found, with conserved exon-intron positions and junction sequences. The B. oleae ace locus extends for at least 75 kb, consists of ten exons with nine introns and is mapped to division 34 of the chromosome arm IIL. Moreover, according to bioinformatic analysis, the Bo AChE exhibits all the common features of the insect AChE. Such structural and functional similarity among closely related AChE enzymes may implicate similarities in insecticide resistance mechanisms.

Type
Research Paper
Copyright
Copyright © Cambridge University Press 2012

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