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Resolving multiple host use of an emergent pest of cotton with microsatellite data and chloroplast markers (Creontiades dilutus Stål; Hemiptera, Miridae)

Published online by Cambridge University Press:  23 May 2013

James P. Hereward ,
Paul J. DeBarro  and
Gimme H. Walter
James P. Hereward*
Affiliation:
School of Biological Sciences, The University of Queensland, Brisbane, Queensland, Australia Cotton Catchment Communities Cooperative Research Centre, Australian Cotton Research Institute, Narrabri, New South Wales, Australia
Paul J. DeBarro
Affiliation:
CSIRO Ecosystem Sciences, GPO Box 2583, Brisbane, Queensland, Australia
Gimme H. Walter
Affiliation:
School of Biological Sciences, The University of Queensland, Brisbane, Queensland, Australia
*
*Author for correspondence Phone: +61 73365 2755 Fax: N/A E-mail: j.hereward@uq.edu.au
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Abstract

Following the global uptake of transgenic cotton several Hemipteran pests have emerged as primary targets for pesticide control. Previous research on one such emergent pest: the green mirid, Creontiades dilutus, indicated differential use of two crop hosts, cotton (Gossypium hirsutum, Malvaceae) and lucerne (alfalfa) (Medicago sativa, Fabaceae). We tested the hypothesis that this apparent demographic independence of lucerne and cotton inhabiting mirids is the result of cryptic species being associated with these two crops. We assessed gene flow using microsatellite markers across adjacent cotton and lucerne crops at three geographically separated sites (up to 900 km apart). We also analysed the recent feeding behaviour of these insects by amplifying chloroplast markers from their gut contents. We find high gene flow between these two crops (mean pair wise FST between host plants=0.0141 within localities), and no evidence of cryptic species. Furthermore, the gut analyses revealed evidence of substantial recent movement between these two crops. We discuss the implications of these results for interpreting multiple host use in this species and setting future research priorities for this economically important pest.

Keywords

Population geneticscryptic speciespolyphagyfeeding ecology
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 103 , Issue 5 , October 2013 , pp. 611 - 618
Copyright
Copyright © Cambridge University Press 2013 

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