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Acute Spinal Cord Injury in the Rat: Comparison of Three Experimental Techniques

Published online by Cambridge University Press:  18 September 2015

Moe Khan  and
Robert Griebel
Moe Khan*
Affiliation:
Department of Clinical Neurological Sciences, Division of Neurosurgery, University of Saskatchewan, Saskatoon
Robert Griebel
Affiliation:
Department of Clinical Neurological Sciences, Division of Neurosurgery, University of Saskatchewan, Saskatoon
*
Dept. of Clinical Neurological Sciences, University Hospital, Saskatoon, Saskatchewan S7N 0X0 Canada.
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Three techniques to produce experimental spinal cord injuries in the rat are compared; 1) the weight dropping method, 2) the aneurysm clip compression method and 3) the extradural balloon compression method. In principle, different forces were used in technique one, while a constant force for different durations is maintained in techniques two and three. The relationship between these different types of injuries and subsequent clinical recovery was assessed quantitatively by the inclined plane method of Rivlin and Tator. The weight dropping technique was found unreliable for experimental spinal cord injury in the rat while the aneurysm clip compression technique resulted in consistent cord injuries with respect to subsequent clinical recovery. The extradural balloon compression method invariably resulted in complete recovery after three and five minutes but no recovery after seven minutes of 0.1 cc air inflated balloon compression of the cord indicating a steep dose — response curve. However, using a 0.2 cc air inflated balloon, no recovery was noted after one minute compression. The major factor in the pathogenesis of spinal cord injury produced by the weight dropping technique is believed to be mechanical, while both mechanical and vascular factors seem to operate in the clip and balloon compression techniques.

Type
Original Articles
Information
Canadian Journal of Neurological Sciences , Volume 10 , Issue 3 , August 1983 , pp. 161 - 165
Copyright
Copyright © Canadian Neurological Sciences Federation 1983

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