Hostname: page-component-8448b6f56d-qsmjn Total loading time: 0 Render date: 2024-04-16T08:50:27.684Z Has data issue: false hasContentIssue false
  • English
  • Français

Quercetin Administration After Spinal Cord Trauma Changes S-100β Levels

Published online by Cambridge University Press:  02 December 2014

E. Schültke ,
R. W. Griebel  and
B. H. J. Juurlink
E. Schültke*
Affiliation:
Department of Anatomy & Cell Biology, University of Saskatchewan, Saskatoon, SK, Canada Department of Surgery, Division of Neurosurgery, University of Saskatchewan, Saskatoon, SK, Canada Department of Stereotactic and Functional Neurosurgery, University of Freiburg, Germany
R. W. Griebel
Affiliation:
Department of Surgery, Division of Neurosurgery, University of Saskatchewan, Saskatoon, SK, Canada
B. H. J. Juurlink
Affiliation:
Department of Anatomy & Cell Biology, University of Saskatchewan, Saskatoon, SK, Canada College of Medicine, Alfaisal University, Riyadh, Kingdom of Saudi Arabia
*
A302 Health Sciences Building, College of Medicine, University of Saskatchewan, 107 Wiggins Road, Saskatoon, Saskatchewan, S7N 5E5, Canada.
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.
Background:

It has been shown previously that S-100β levels in serum correspond with the severity of central nervous system (CNS) trauma. It also has been suggested that S-100β in CNS tissue is involved in neuroprotection and neuroregeneration. We have previously shown that administration of quercetin results in improved motor function in an animal model of spinal cord trauma.

Methods:

Mid-thoracic spinal cord compression injury was produced in adult maleWistar rats. Serum and tissue samples were acquired from quercetin-treated animals (25 μmol / kg) and saline controls at 6, 12 and 24 hours after the trauma. S-100β levels were measured using a luminometric assay in the damaged tissue and in the serum of the animals.

Results:

The increase in serum S-100β levels seen in saline controls after spinal cord trauma was ameliorated in the quercetin-treated animals at all time points, although the difference to saline controls became statistically significant only at 24 hrs after the trauma. Compared to tissue S-100β levels in healthy animals, values were significantly decreased in saline controls at all three time points, while they were decreased at 6 hrs and increased at both 12 and 24 hrs in quercetin-treated animals. At all three time points tissue S-100β levels were significantly higher in quercetin-treated animals than in saline controls.

Conclusions:

Administration of quercetin results in modification of S-100β levels in the setting of experimental spinal cord trauma. The kinetic patterns of the S-100β fluctuations in serum and tissue suggest that post-traumatic administration of quercetin decreases the extent of CNS injury.

Type
Research Article
Information
Canadian Journal of Neurological Sciences , Volume 37 , Issue 2 , March 2010 , pp. 223 - 228
Copyright
Copyright © The Canadian Journal of Neurological 2010

References

1. Steiner, J, Bernstein, HG, Bielau, H, Berndt, A, Brisch, R, Mawrin, C, et al. Evidence for a wide extra-astrocytic distribution of S100B in human brain. BMC Neurosci. 2007;8:2.Google Scholar
2. Adami, C, Sorci, G, Blasi, E, Agneletti, AL, Bistoni, F, Donato, R. S100B expression in and effects on microglia. Glia. 2001;33(2): 131–42.Google Scholar
3. Townend, W, Dibble, C, Abid, K, Vail, A, Sherwood, R, Lecky, F. Rapid elimination of protein S-100B from serum after minor head trauma. J Neurotrauma. 2006;23(2):149–55.Google Scholar
4. Raabe, A, Grolms, C, Keller, M, Döhnert, J, Sorge, O, Seifert, V. Correlation of computed tomography findings and serum brain damage markers following severe head injury. Acta Neurochir. (Wien) 1998;140(8):791–2.CrossRefGoogle ScholarPubMed
5. Ingebrigtsen, T, Waterloo, K, Jacobsen, EA, Langbakk, B, Romner, B. Traumatic brain damage in minor head injury: relation of serum S-100 protein measurements to magnetic resonance imaging and neurobehavioral outcome. Neurosurgery. 1999;45(3):475–6.CrossRefGoogle ScholarPubMed
6. Woertgen, C, Rothoerl, RD, Metz, C, Brawanski, A. Comparison of clinical, radiologic and serum marker as prognostic factors after severe head injury. J Trauma. 1999;47(6):1126–30.CrossRefGoogle ScholarPubMed
7. Herrmann, M, Jost, S, Kutz, S, Ebert, AD, Kratz, T, Wunderlich, MT, et al. Temporal profile of release of neurobiochemical markers of brain damage after traumatic brain injury is associated with intracranial pathology as demonstrated in cranial computerized tomography. J Neurotrauma. 2000;17(2):113–22.Google Scholar
8. Romner, B, Ingebrigtsen, T, Kongstad, P, Borgesen, SE. Traumatic brain damage: serum S-100 protein measurements related to neuroradiolagical findings. J. Neurotrauma. 2000;17(8):641–7.Google Scholar
9. Poli-de-Figueiredo, LF, Biberthaler, P, Simao Filho, C, Hauser, C, Mutschler, W, Jochum, M. Measurement of S-100B for risk classification of victims sustaining minor head injury-first pilot study in Brazil. Clinics. 2006;61(1):41–6.Google Scholar
10. Thorngren-Jerneck, K, Ohlsson, T, Sandell, A, Erlandsson, K, Strand, SE, Ryding, E, et al. Cerebral glucose metabolism measured by positron emission tomography in term newborn infants with hypoxic ischemic encephalopathy. Pediatr Res. 2001;49(4): 495–501.Google Scholar
11. Raabe, A, Grolms, C, Sorge, O, Zimmermann, M, Seifert, V. Serum S-100 protein in severe head injury. Neurosurgery. 1999;45(3): 477–83.CrossRefGoogle ScholarPubMed
12. Raabe, A, Grolms, C, Seifert, V. Serum markers of brain damage and outcome prediction in patients after severe head injury. Br J Neurosurg. 1999;13(1):56–9.Google Scholar
13. Raabe, A, Seifert, V. Fatal secondary increase in serum S-100β protein after severe head injury. Report of three cases. J Neurosurg. 1999;91(5):875–7.Google Scholar
14. Rothoerl, RD, Woertgen, C, Holzschuh, M, Metz, C, Brawanski, A. S-100 serum levels after minor and major head injury. J Trauma. 1998;45(4):765–7.Google Scholar
15. Woertgen, C, Rothoerl, RD, Holzschuh, M, Metz, C, Brawanski, A. Comparison of serial S-100 and NSE serum measurements after severe head injury. Acta Neurochir. (Wien) 1997;139(12): 1161–5.Google Scholar
16. Khaladj, N, Teebken, OE, Hagl, C, Wilhelmi, MH, Tschan, C, Weissenborn, K, et al. The role of cerebrospinal fluid S100 and lactate to predict clinically evident spinal cord ischaemia in thoraco-abdominal aortic surgery. Eur J Vasc Endovasc Surg. 2008;36(1):11–9.Google Scholar
17. Kunihara, T, Shiiya, N, Yasuda, K. Changes in S100beta protein levels in cerebrospinal fluid after thoracoabdominal aortic operations. J Thorac Cardiovasc Surg. 2001;122(5):1019–20.Google Scholar
18. van Dongen, EP, Ter Beek, HT, Boezeman, EH, Schepens, MA, Langemeijer, HJ, Aarts, LP. Normal serum concentrations of S-100 protein and changes in cerebrospinal fluid concentrations of S-100 protein during and after thoracoabdominal aortic aneurysm surgery: Is S-100 protein a biochemical marker of clinical value in detecting spinal cord ischemia? J Vasc Surg. 1998;27(2):344–6.Google Scholar
19. Cao, F, Yang, XF, Liu, WG, Hu, WW, Li, G, Zheng, XJ, et al. Elevation of neuron-specific enolase and S-100beta protein level in experimental acute spinal cord injury. J Clin Neurosci. 2008;15(5):541–4.Google Scholar
20. do Carmo Cunha, J, de Freitas Azevedo Levy, B, de Luca, BA, de Andrade, MS, Gomide, VC, Chadi, G. Responses of reactive astrocytes containing S100beta protein and fibroblast growth factor-2 in the border and in the adjacent preserved tissue after a contusion injury of the spinal cord in rats: implications for wound repair and neuroregeneration. Wound Repair Regen. 2007;15(1):134–46.Google Scholar
21. Loy, DN, Sroufe, AE, Pelt, JL, Burke, DA, Cao, QL, Talbott, JF, et al. Serum biomarkers for experimental acute spinal cord injury: rapid elevation of neuron-specific enolase and S-100beta. Neurosurgery. 2005;56(2):391–7.Google Scholar
22. Schültke, E, Kendall, EJ, Kamencic, H, Ghong, Z, Griebel, RW, Juurlink, BHJ. MRI supported dose determination: an animal study on the efficacy of quercetin dihydrate in acute spinal cord injury. J Neurotrauma. 2003;20(6):583–91.Google Scholar
23. Schültke, E, Kamencic, H, Zhao, M, Tian, GF, Baker, AJ, Griebel, RW, et al. Neuroprotection following fluid percussion brain trauma: A pilot study using quercetin. J Neurotrauma. 2005;22(12):1475–84.Google Scholar
24. Rivlin, AS, Tator, CH. Effect of duration of acute spinal cord compression in a new acute injury model in the rat. Surg Neurol. 1978;10:38–43.Google Scholar
25. Raabe, A, Kopetsch, O, Gross, U, Zimmermann, M, Gebhart, P. Measurements of serum S-100B protein: effects of storage time and temperature on pre-analytical stability. Clin Chem Lab Med. 2003;41(5):700–3.Google Scholar
26. Nishi, M, Kawata, M, Azmitia, EC. S100beta promotes the extension of microtubule associated protein2 (MAP2)-immunoreactive neurites retracted after colchicine treatment in rat spinal cord culture. Neurosci Lett. 1997;229(3):212–4.Google Scholar
27. Iwasaki, Y, Shiojima, T, Kinoshita, M. S100 beta prevents the death of motor neurons in newborn rats after sciatic nerve section. J Neurol Sci. 1997;151(1):7–12.CrossRefGoogle ScholarPubMed
28. Haglid, KG, Yang, Q, Hamberger, A, Bergman, S, Widerberg, A, Danielsen, N. S-100beta stimulates neurite outgrowth in the rat sciatic nerve grafted with acellular muscle transplants. Brain Res. 1997;753(2):196–201.Google Scholar
29. Liu, JB, Tang, TS, Yang, HL. Antioxidation of quercetin against spinal cord injury in rats. Clin J Trauma. 2006;9(5):303–7.Google ScholarPubMed
30. Jackson, RG, Samra, GS, Radcliffe, J, Clark, GH, Price, CP. The early fall in levels of S-100 beta in traumatic brain injury. Clin Chem Lab Med. 2000;38(11):1165–7.Google Scholar