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Secondary Epileptogenesis in Frog Forebrain: Effect of Inhibition of Protein Synthesis

Published online by Cambridge University Press:  18 September 2015

F. Morrell ,
N. Tsuru ,
T.J. Hoeppner ,
D. Morgan  and
W.H. Harrison
F. Morrell*
Affiliation:
Dept. of Neurological Sciences, Rush Medical College, Rush-Presbyterian - St. Luke's Medical Center, Chicago, III. 60612 and Marine Biological Laboratory, Woods Hole, Mass. 02543
N. Tsuru
Affiliation:
Dept. of Neurological Sciences, Rush Medical College, Rush-Presbyterian - St. Luke's Medical Center, Chicago, III. 60612 and Marine Biological Laboratory, Woods Hole, Mass. 02543
T.J. Hoeppner
Affiliation:
Dept. of Neurological Sciences, Rush Medical College, Rush-Presbyterian - St. Luke's Medical Center, Chicago, III. 60612 and Marine Biological Laboratory, Woods Hole, Mass. 02543
D. Morgan
Affiliation:
Dept. of Neurological Sciences, Rush Medical College, Rush-Presbyterian - St. Luke's Medical Center, Chicago, III. 60612 and Marine Biological Laboratory, Woods Hole, Mass. 02543
W.H. Harrison
Affiliation:
Dept. of Neurological Sciences, Rush Medical College, Rush-Presbyterian - St. Luke's Medical Center, Chicago, III. 60612 and Marine Biological Laboratory, Woods Hole, Mass. 02543
*
Rush-Presbyterian - St. Lukes Medical Center, Chicago, 111, 60612 U.S.A.
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Secondary epileptogenesis was induced in the hippocampal cortex of the paralyzed bullfrog by means of localized, unilateral, intermittent electrical stimulation (kindling). Stimuli were designed to yield a brief but definite after-discharge. In control animals a progressive increase in after-discharge duration occurred at the 1° (stimulated) site and then at the 2° site (contralateral hippocampus). Spontaneous epileptiform potentials (SEP's) occurred between stimuli, eventually independently on both sides.

Cycloheximide (50 mg/kg) caused 88-99% reduction in protein synthesis, measured by 14C-leucine incorporation into brain protein. Cycloheximide-treated animals revealed no evidence of progressive prolongation of after-discharge duration when subjected to the kindling procedure (p = 0.1205xl0-7). SEP's were reduced in the cycloheximide-treated animals, and confined to 1° hemisphere (p=0.6xl0-10).

Since cycloheximide did not disturb normal electrogenesis or disrupt the after-discharges, this experiment distinguishes processes dependent upon electrical events from those requiring macromolecular synthesis. Protein synthesis seems critical to the emergence of spontaneous and autonomous epileptiform behavior of neural aggregates.

Type
Research Article
Information
Canadian Journal of Neurological Sciences , Volume 2 , Issue 4 , November 1975 , pp. 407 - 416
Copyright
Copyright © Canadian Neurological Sciences Federation 1975

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