Hostname: page-component-848d4c4894-wzw2p Total loading time: 0 Render date: 2024-05-15T07:11:41.745Z Has data issue: false hasContentIssue false
  • English
  • Français

Suppressive Effects of Various Amino Acids against Ouabain-Induced Seizures in Rats

Published online by Cambridge University Press:  18 September 2015

Y. Tsukada ,
N. Inoue ,
J. Donaldson  and
A. Barbeau
Y. Tsukada*
Affiliation:
Department of Neurobiology, Clinical Research Institute of Montreal, 110 Pine Avenue West, Montreal, Quebec, Canada
N. Inoue
Affiliation:
Department of Neurobiology, Clinical Research Institute of Montreal, 110 Pine Avenue West, Montreal, Quebec, Canada
J. Donaldson
Affiliation:
Department of Neurobiology, Clinical Research Institute of Montreal, 110 Pine Avenue West, Montreal, Quebec, Canada
A. Barbeau
Affiliation:
Department of Neurobiology, Clinical Research Institute of Montreal, 110 Pine Avenue West, Montreal, Quebec, Canada
*
Clinical Research Institute of Montreal, 110 Pine Avenue West, Montreal, Quebec, Canada H2W 1R7
Rights & Permissions [Opens in a new window]

Summary:

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The suppressive effect of various amino acids against ouabaininduced seizures was investigated in young female rats. The amino acids were injected into the left lateral ventricle 10 minutes prior to the intraventricular administration of 5 μg. of ouabain. Animals receiving 1.9 x 10-1 M solutions of hypotaurine and of β- alanine were almost completely protected from the ouabain seizures. Administration of L-alanine and of glycine was also effective, although running and leaping seizures still occurred to some extent. Betaine reduced only clonic-tonic and whole body flexion and extension seizures. In contrast, L-proline exclusively suppressed clonic-tonic and focal clonic seizures. Rats injected with isethionic acid showed increases in incidence of running and leaping seizures while L-arginine in high concentrations caused aggravation in clonic-tonic seizures. L-cysteine, even in low concentrations, also brought about an increase in the occurrence and incidence of clonic-tonic seizures. The ED50of hypotaurine was 10.11 x 10-2 M for running seizures and 4.63 x 10-2, M for clonictonic seizures; that of β -alanine was 14.01 x 10-2 M for running seizures and 5.50 x 10-2 M for clonic-tonic seizures. However, hypotaurine and β -alanine, the most effective compounds tested in the present studies, provided less protection than taurine previously examined by us under similar conditions (Izumi et al., 1973).

Type
Research Article
Information
Canadian Journal of Neurological Sciences , Volume 1 , Issue 4 , November 1974 , pp. 214 - 221
Copyright
Copyright © Canadian Neurological Sciences Federation 1974

References

REFERENCES

Alberti, K.G., Shahriari, A.A., Levine, H.D., and Lauler, D.P. (1971). Reversal of ouabain-induced arrhythmias in the dog by intravenous arginine hydrochloride. Cardiovascular Research, 5, 226–235.CrossRefGoogle ScholarPubMed
Balcar, V.J., and Johnston, A.R. (1973). High affinity uptake of transmitters: Studies on the uptake of L-aspartate, GABA, L-glutamate and glycine in cat spinal cord. Journal of Neurochemistry, 20, 529–539.CrossRefGoogle ScholarPubMed
Barbeau, A., and Donaldson, J. (1974). Zinc, taurine and epilepsy. Archives of Neurology, 30, 52–58.CrossRefGoogle ScholarPubMed
Barker, J.L., and Nicoll, R.A. (1973). The pharmacology and ionic dependency of amino acid responses in the frog spinal cord. Journal of Physiology, 228, 259–277.CrossRefGoogle ScholarPubMed
Barkulis, S.S., Geiger, A., Kawakita, Y., and Aguilar, V. (1960). A study on the incorporation of 14C derived from glucose into the free amino acids of the brain cortex. Journal of Neurochemistry, 5, 339–348.CrossRefGoogle Scholar
Boineau, F. (1973). Effect of penicillamine and penicillin on calcium and magnesium levels of escherichia coli K12. Bordeaux Medical, 6, 1115–1127, (from Chem. Abst. 79 (1973) 38882h).Google Scholar
Cuŕtis, D.R., and Crawford, J.M. (1969). Central synaptic transmission - Microelectrophoretic studies. Annual Revue of Pharmacology, 9, 209–240.CrossRefGoogle ScholarPubMed
De Belleroche, J.S., and Bredford, H.F. (1973). Amino acids in synaptic vesicles from mammalian cerebral cortex: A reappraisal. Journal of Neurochemistry, 21, 441–451.CrossRefGoogle ScholarPubMed
Dolara, P., Marino, P., and Buffoni, F. (1973). Effect of 2-amino ethanesulphonic acid (taurine) and 2-hydroxyethane sulphonic acid (isethionic acid) on calcium transport by rat liver mitochondria. Biochemical Pharmacology, 22, 2085–2094.CrossRefGoogle Scholar
Dolara, P., Agresti, A., Giotti, A., and Pasquini, G. (1973). Effect of taurine on calcium kinetics of guinea-pig heart. European Journal of Pharmacology, 24, 352–358.CrossRefGoogle ScholarPubMed
Donaldson, J., St-Pierre, T., Minnich, J., and Barbeau, A. (1971). Seizures in rats associated with divalent cation inhibition of Na+-K+-ATP’ase. Canadian Journal of Biochemistry, 49, 1217–1224.CrossRefGoogle ScholarPubMed
Dunn, A., and Giudetta, A. (1971). A long term effect of electroconvulsive shock on the metabolism of glucose in mouse brain. Brain Research, 27, 418–421.CrossRefGoogle Scholar
Ehinger, B., and Falck, B. (1971). Autoradiography of some suspected neurotransmitter substances GABA, glycine, glutamic acid, histamine, dopamine and L-DOPA. Brain Research, 33, 157–172.CrossRefGoogle ScholarPubMed
Ehinger, B. (1972). Cellular location of uptake of some amino acids into the rabbit retina. Brain Research, 46, 297–311.CrossRefGoogle ScholarPubMed
Goldstein, A. (1964). Biostatistics. The Macmillan Co., New York, p. 51.Google Scholar
Greenstein, J.P., and Winitz, M. (1961). Metal and salt combinations with amino acids. In: Chemistry of the amino acids, John Wiley and Sons, Inc., New York — London, Vol. 1, p. 569.Google Scholar
Hahashi, T. (1972). In: Comprehensive management of epilepsy in infancy, childhood and adolescence. (Livingston, S., ed.), Thomas, C.C, Springfield, Illinois, pp. 313–314.Google Scholar
Huxtable, L., and Bressler, B. (1973). Effect of taurine on muscle intercellular membrane. Biochem. Biophys. Acta., 323, 573–583.CrossRefGoogle Scholar
Iversen, L.L., and Schon, F.E. (1973). The use of autoradiographic techniques for the identification and mapping of transmitter - specific neurons in CNS. In: “New concepts in neurotransmitter regulation” (Mandell, A.J. Ed.), Plenum Press, New York, pp. 153–194.CrossRefGoogle Scholar
Izumi, K., Donaldson, J., Minnich, J.J., and Barbeau, A. (1973). Ouabaininduced seizures in rats: Suppressive effects of taurine and γ -aminobutyric acid. Canadian Journal of Physiology and Pharmacology, 51, 885–889.CrossRefGoogle ScholarPubMed
Jones, D.A., and Mcilwain, H. (1971). Amino acid distribution and incorporation into protein in isolated electricallystimulated cerebral tissues. Journal of Neurochemistry, 18, 41–58.CrossRefGoogle ScholarPubMed
Kaczmarek, L.K., and Davison, A.N. (1972). Uptake and release of taurine from rat brain slices. Journal of Neurochemistry, 19, 2355–2362.CrossRefGoogle ScholarPubMed
Kaczmarek, L.K., and Adey, W.R. (1973). The efflux of 45C2+ and (3H)γ aminobutyric acid from; cat cerebral cortex. Brain Research, 63, 331–342.CrossRefGoogle Scholar
Koyama, I. (1972). Amino acids in the cobalt-induced epileptogenic and non epileptogenic cat’s cortex. Canadian Journal of Physiology and Pharmacology, 50, 740–752.CrossRefGoogle Scholar
Krnjevic, K., and Lisiewicz, A. (1972). Injections of calcium ions into spinal motoneurones. Journal of Physiology, 225, 363–390.CrossRefGoogle ScholarPubMed
Lähdesmäki, P., and Oja, S.S. (1973). On the mechanism of taurine transport at brain cell membranes. Journal of Neurochemistry, 20, 1411–1417.CrossRefGoogle ScholarPubMed
Litchfield, J.T., and Wilcoxon, F. (1949). A simplified method of evaluating dose-effect experiments. Journal of Pharmacology and Experimental Therapeutics, 96, 99–113.Google ScholarPubMed
Nahorski, S.R., Roberts, D.J., and Stewart, G.G. (1970). Some neurochemical aspects of pentamethylenetetrazole convulsive activity in rat brain. Journal of Neurochemistry, 17, 621–631.CrossRefGoogle ScholarPubMed
Obata, K., and Yoshida, M. (1973). Caudate-evoked inhibition and actions of GABA and other substances on cat pallidal neurones. Brain Research, 64, 455–459.CrossRefGoogle Scholar
Oja, S.S., Karvonen, M.L., and Lähdesmäki, P. (1973). Biosynthesis of taurine and enhancement of decarboxylation of cysteine sulphinate and glutamate by the electrical stimulation of rat brain slices. Brain Research, 55, 173–178.CrossRefGoogle ScholarPubMed
Orrego, F., and Lipmann, F. (1967). Protein synthesis in brain slices. Effects of electrical stimulation and acidic amino acids. Journal of Biological Chemistry, 242, 665–671.CrossRefGoogle ScholarPubMed
Perry, T.L., and Hansen, S. (1973). Quantification of free amino compounds of rat brain: Identification of hypotaurine. Journal of Neurochemistry, 21, 1009–1011.CrossRefGoogle ScholarPubMed
Reynolds, A.P., and Gallagher, B.B. (1973). The effect of hexafluorodiethyl ether (Flurothyl) on the metabolism of rat brain amino acids labelled by (U-14C) glucose. Life Science, 13, 87–95.CrossRefGoogle Scholar
Roberge, A., and Charbonneau, R. (1969). Protective effects of arginine against hepatic coma, insulin shock and epilepsy. Life Science, 8, 369–377.CrossRefGoogle ScholarPubMed
Rubin, P. (1970). The role of calcium in the release of neural transmitter substances and hormones. Pharmacology Revue, 22, 389–428.Google Scholar
Snodgrass, S.R., and Lorenzo, A.V. (1973). Transport of GABA from the perfused ventricular system of the cat. Journal of Neurochemistry, 20, 761–769.CrossRefGoogle ScholarPubMed
Snyder, S.H., Young, A.B., Bennett, J.P., and Mulder, A.H. (1973). Synaptic biochemistry of amino acids. Federation Proceedings, 32, 2039–2047.Google ScholarPubMed
Snyder, S.H., Yamamura, H.I., Pert, C.B., Logan, W.J., and Bennett, J.P. (1973). Neuronal uptake of neurotransmitters and their precursors Studies with “transmitter” amino acids and choline. In: (Mandell, A.J., ed.). New concepts in neurotransmitter regulation. Plenum Press, New York — London, pp. 195–222.CrossRefGoogle Scholar
Van Gelder, N.M., Sherwin, A.L., and Rasmussen, T. (1972). Amino acid content of epileptogenic human brain: Focal versus surrounding regions. Brain Research, 40, 385–393.CrossRefGoogle ScholarPubMed
Van Gelder, N.M. (1972). Antagonism by taurine of cobalt induced epilepsy in cat and mouse. Brain Research, 47, 157–165.CrossRefGoogle ScholarPubMed
Welty, J.D, and Read, W.O. (1964). Studies on some cardiac effects of taurine. Journal of Pharmacology and Experimental Therapeutics, 144, 110–115.Google ScholarPubMed
Winer, B.J., (1971). Statistical principles in experimental design. 2nd Edition, McGraw-Hill Book Co., New York.Google Scholar
Zuckermann, E.C., and Glaser, G.H. (1973). Anticonvulsive action of increased calcium concentration in cerebrospinal fluid. Archives of Neurology, 29, 245–252.CrossRefGoogle ScholarPubMed
Zuckermann, E.C., and Glaser, G.H. (1968). Hippocampal epileptic activity induced by localized ventricular perfusion with high potassium cerebrospinal fluid. Experimental Neurology, 20, 87–110.CrossRefGoogle ScholarPubMed