Hostname: page-component-76dd75c94c-lntk7 Total loading time: 0 Render date: 2024-04-30T07:24:00.266Z Has data issue: false hasContentIssue false
  • English
  • Français

1175 – The Effect Of Mk-801 On The Erk-creb Signal System In Prenatally Stressed Offspring Rats

Published online by Cambridge University Press:  15 April 2020

L. Guan ,
X. Zhao ,
X. Zhang ,
H. Sun ,
D. Wang ,
Q. Su ,
G. Tang ,
L. Yang ,
Q. Song  and
N. Jia
...Show all authors
L. Guan
Affiliation:
Department of Pharmacology, Xi’an Jiaotong University, Xi’an, China
X. Zhao
Affiliation:
Department of Pharmacology, Xi’an Jiaotong University, Xi’an, China
X. Zhang
Affiliation:
Department of Pharmacology, Xi’an Jiaotong University, Xi’an, China
H. Sun
Affiliation:
Department of Pharmacology, Xi’an Jiaotong University, Xi’an, China
D. Wang
Affiliation:
Department of Biology, Northwest University, Xi’an, China
Q. Su
Affiliation:
Department of Neonatology, Xi’an, China
G. Tang
Affiliation:
Department of Biology, Northwest University, Xi’an, China
L. Yang
Affiliation:
Department of Neonatology, Xi’an, China
Q. Song
Affiliation:
Department of Biology, Northwest University, Xi’an, China
N. Jia
Affiliation:
Department of Human Anatomy and Embryology, Xi’an Jiaotong University, Xi’an, China
H. Li
Affiliation:
Department of Neonatology, First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
Z. Zhu
Affiliation:
Department of Pharmacology, Xi’an Jiaotong University, Xi’an, China Department of Biology, Northwest University, Xi’an, China

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.
Introduction

Studies revealed that prenatal stress (PS) may increase the vulnerability to depression in their offspring, and ERK-CREB signal system might play a role in its mechanism.

Objectives and aims

The present study investigated the effect of MK-801 on depressive-like behavior and its impacts on ERK2, CREB, Bcl-2 mRNA expression in PS female rat offspring.

Methods

The pregnant rats were randomly divided into three groups, the control group (Con) was left undisturbed, the PS-saline group (PS-saline) and the PS-MK-801 group (PS-MK-801) were subjected to restraint stress on days 14–20 of pregnancy three times daily for 45 min, and received an i.p. administration of saline or MK-801(sigma, 0.2 mg/kg) 30 min before the first stress respectively. Forced swimming test was undertaken to assess depressive-like behavior in one month female offspring. ERK2, CREB, Bcl-2 mRNA in the hippocampus, frontal cortex, and striatum were detected by RT-PCR.

Results

PS-saline spent significantly more immobile time compared to Con and PS-MK-801 (P < 0.05). ERK2 and CREB mRNA expression in hippocampus and frontal cortex was significantly decreased in PS-saline compared to Con and PS-MK-801 (P < 0.05), while in striatum CREB mRNA expression in PS-saline was lower than Con (P < 0.05). Bcl-2 mRNA expression in hippocampus and striatum was significantly decreased in PS-saline (P < 0.05), and in frontal cortex, its expression was significantly lower in PS-saline and PS-MK-801 (P < 0.05).

Conclusions

PS may suppress ERK-CREB signal pathway in female offspring rats, which could be partly prevented by MK- 801. (Supported by National Natural Science Foundation of China, No: 30970952).

Type
Abstract
Information
European Psychiatry , Volume 28 , Issue S1: Abstracts of the 21th European Congress of Psychiatry , 2013 , 28-E549
Copyright
Copyright © European Psychiatric Association 2013
Submit a response

Comments

No Comments have been published for this article.