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Impact of assembly, testing and launch operations on the airborne bacterial diversity within a spacecraft assembly facility clean-room

  • David A. Newcombe (a1) (a2), Myron T. La Duc (a1), Parag Vaishampayan (a1) and Kasthuri Venkateswaran (a1)

In an effort to minimize the probability of forward contamination of pristine extraterrestrial environments, the National Aeronautics and Space Administration requires that all US robotic spacecraft undergo assembly, testing and launch operations (ATLO) in controlled clean-room environments. This study examines the impact of ATLO activity on the microbial diversity and overall bioburden contained within the air of the clean-room facility in which the Mars Exploration Rovers (MERs) underwent final preparations for launch. Air samples were collected from several facility locations and traditional culture-based and molecular methodologies were used to measure microbial burden and diversity. Surprisingly, the greatest estimates of airborne bioburden, as derived from ATP content and cultivation assays, were observed prior to the commencement of MER ATLO activities. Furthermore, airborne microbial diversity gradually declined from the initiation of ATLO on through to launch. Proteobacterial sequences were common in 16S rDNA clone libraries. Conspicuously absent were members of the Firmicutes phylum, which includes the genus Bacillus. In previous studies, species of this genus were repeatedly isolated from the surfaces of spacecraft and clean-room assembly facilities. Increased cleaning and maintenance initiated immediately prior to the start of ATLO activity could explain the observed declines in both airborne bioburden and microbial diversity.

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D.G. Ahearn , S.A. Crow , R.B. Simmons , D.L. Price , S.K. Mishra & D.L. Pierson (1997). Fungal colonization of air filters and insulation in a multi-story office building: production of volatile organics. Curr. Microbiol. 35, 305308.

J. Appelbaum & D.J. Flood (1990). Solar-Radiation on Mars. Sol. Energy 45, 353363.

V.A. Castro , A.N. Thrasher , M. Healy , C.M. Ott & D.L. Pierson (2004). Microbial characterization during the early habitation of the International Space Station. Microb. Ecol. 47, 119126.

E.A. Christensen , P. Gerner-Smidt & H. Kristensen (1991). Radiation resistance of clinical Acinetobacter spp.: a need for concern? J. Hosp. Infect. 18, 8592.

T. Coenye & P. Vandamme (2003). Diversity and significance of Burkholderia species occupying diverse ecological niches. Environ. Microbiol. 5, 719729.

R.L. Crawford (2005). Microbial diversity and its relationship to planetary protection. Appl. Environ. Microbiol. 71, 41634168.

I.J. Good (1953). The population frequencies of species and the estimation of population parameters. Biometrika 40, 237264.

J.K. Heck , G. van Belle & D. Simberloff (1975). Explicit calculation of the rarefaction diversity measurement and the determination of sufficient sample size. Ecology 56, 14591461.

A. Jawad , A.M. Snelling , J. Heritage & P.M. Hawkey (1998). Exceptional desiccation tolerance of Acinetobacter radioresistens. J. Hosp. Infect. 39, 235240.

M.J. Kempf , F. Chen , R. Kern & K. Venkateswaran (2005). Recurrent isolation of hydrogen peroxide-resistant spores of Bacillus pumilus from a spacecraft assembly facility. Astrobiology 5, 391405.

A. Kramer , I. Schwebke & G. Kampf (2006). How long do nosocomial pathogens persist on inanimate surfaces? A systematic review. BMC Infect. Dis. 6, 130137.

M.T. La Duc , A. Dekas , S. Osman , C. Moissl , D. Newcombe & K. Venkateswaran (2007a). Isolation and characterization of bacteria capable of tolerating the extreme conditions of clean room environments. Appl. Environ. Microbiol. 73, 26002611.

M.T. La Duc , R. Kern & K. Venkateswaran (2004a). Microbial monitoring of spacecraft and associated environments. Microbial. Ecol. 47, 150158.

M.T. La Duc , W. Nicholson , R. Kern & K. Venkateswaran (2003). Microbial characterization of the Mars Odyssey spacecraft and its encapsulation facility. Environ. Microbiol. 5, 977985.

M.T. La Duc , M. Satomi , N. Agata & K. Venkateswaran (2004b). gyrB as a phylogenetic discriminator for members of the Bacillus anthracis-cereus-thuringiensis group. J. Microbiol. Methods 56, 383394.

M. La Duc , T. Stucker & K. Venkateswaran (2007b). Molecular bacterial diversity and bioburden of commercial airliner cabin air. Can. J. Microbiol. 53, 12591271.

B. Lawley , S. Ripley , P. Bridge & P. Convey (2004). Molecular analysis of geographic patterns of eukaryotic diversity in Antarctic soils. Appl. Environ. Microbiol. 70, 59635972.

M.L. Ledrich , S. Stemmler , P. Laval-Gilly , L. Foucaud & J. Falla (2005). Precipitation of silver-thiosulfate complex and immobilization of silver by Cupriavidus metallidurans CH34. Biometals 18, 643650.

C. Lozupone , M. Hamady & R. Knight (2006). UniFrac – an online tool for comparing microbial community diversity in a phylogenetic context. BMC Bioinformatics 7, 371.

C. Lozupone & R. Knight (2005). UniFrac: a new phylogenetic method for comparing microbial communities. Appl. Environ. Microbiol. 71, 82288235.

C. Moissl , M.T. La Duc , S. Osman , A.E. Dekas & K. Venkateswaran (2007b). Molecular bacterial community analysis of clean rooms where spacecraft are assembled. FEMS Microbiol. Ecol. 61, 509521.

D.A. Newcombe , A.C. Schuerger , J.N. Benardini , D. Dickinson , R. Tanner & K. Venkateswaran (2005). Survival of spacecraft-associated microorganisms under simulated martian UV irradiation. Appl. Environ. Microbiol. 71, 81478156.

S. Osman , M.T.L. Duc , A. Dekas , D. Newcombe & K. Venkateswaran (2008a). Microbial bioburden and diversity of commercial airline cabin air during short- and long-duration of travel. ISME J. 2, 482497.

S. Osman , Z. Peeters , M.T. La Duc , R. Mancinelli , P. Ehrenfreund & K. Venkateswaran (2008b). Effect of shadowing on the survival of bacteria to conditions simulating Martian atmosphere and UV-radiation. Appl. Environ. Microbiol. 74, 959970.

M.R. Patel , J.C. Zarnecki & D.C. Catling (2002). Ultraviolet radiation on the surface of Mars and the Beagle 2 UV sensor. Planetary Space Sci. 50, 915927.

L. Poirel , S. Figueiredo , V. Cattoir , A. Carattoli & P. Nordmann (2008). Acinetobacter radioresistens as a silent source of carbapenem resistance for Acinetobacter spp. Antimicrob. Agents Chemother. 52, 12521256.

G. Rontó , A. Bérces , H. Lammer , C.S. Cockell , G.J. Molina-Cuberos , M.R. Patel & F. Selsis (2003). Solar UV Irradiation Conditions on the Surface of Mars. Photochem. Photobiol. 77, 3440.

R. Rossello-Mora & R. Amann (2001). The species concept for prokaryotes. FEMS Microbiol. Rev. 25, 3967.

R. Ruimy , V. Breittmayer , P. Elbaze , B. Lafay , O. Boussemart , M. Gauthier & R. Christen (1994). Phylogenetic analysis and assessment of the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas deduced from small-subunit rRNA sequences. Int. J. Syst. Bacteriol. 44, 416426.

J.D. Rummel (1992). Planetary Protection Policy (U.S.A.). Adv. Space Res. 12, 129131.

P.D. Schloss & J. Handelsman (2005). Introducing DOTUR, a computer program for defining operational taxonomic units and estimating species richness. Appl. Environ. Microbiol. 71, 15011506.

R.B. Simmons , D.L. Price , J.A. Noble , S.A. Crow & D.G. Ahearn (1997). Fungal colonization of air filters from hospitals. Am. Ind. Hyg. Assoc. J. 58, 900904.

E. Stackebrandt & B.M. Goebel (1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Syst. Evol. Microbiol. 44, 846849.

K. Venkateswaran , N. Hattori , M.T. La Duc & R. Kern (2003). ATP as a biomarker of viable microorganisms in clean-room facilities. J. Microbiol. Meth. 52, 367377.

K. Venkateswaran , M. Satomi , S. Chung , R. Kern , R. Koukol , C. Basic & D. White (2001). Molecular microbial diversity of a spacecraft assembly facility. Syst. Appl. Microbiol. 24, 311320.

M.C. Verdenelli , C. Cecchini , C. Orpianesi , G.M. Dadea & A. Cresci (2003). Efficacy of antimicrobial filter treatments on microbial colonization of air panel filters. J. Appl. Microbiol. 94, 915.

G. Walzer , E. Rosenberg & E.Z. Ron (2006). The Acinetobacter outer membrane protein A (OmpA) is a secreted emulsifier. Environ. Microbiol. 8, 10261032.

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International Journal of Astrobiology
  • ISSN: 1473-5504
  • EISSN: 1475-3006
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