From metabolism experiments carried out on the pigs enumerated in the first paper of this series the following conclusions are drawn:

(a) The metabolism in a state of inanition appears to be more a function of a power of the weight than of the true surface area as determined by the photographic method.

(b) Hogs born in the summer and autumn of one year appear to show two maxima of metabolism; one almost immediately, as has been observed previously, and another during the following summer, provisionally ascribed to the effect of light on thyroid activity, produced by the intermediary action of the anterior pituitary.

(c) Actual, and theoretically computed average growths are shown to agree remarkably well in the case of eleven of the pigs which were treated in an exactly similar manner. Individual growth curves showed no such agreement. This is considered to strengthen the evidence for the writer's previously stated view that nett energy is a statistical rather than a physiological constant.

1. Following an examination of weaning weight with respect to litter size, it is concluded that no general relation between the two exists. Although a significant regression of weight on litter size was found, it appears probable that in herds where the relation between fertility and milk yield is different, such a regression will not necessarily be found.

2. A sampling experiment was carried out to determine the extent to which a sample might be expected to represent the whole litter. A correlation of 0·96 was found between the mean growth rate of samples consisting of the four pigs nearest the average at weaning and the mean of the whole litter. This represents a loss of 8 per cent of the information. With three or two pigs chosen in the same way the loss was greater. Samples of four pigs chosen at random did not give results significantly different from those of the four pigs nearest the average. The choice of the heaviest four pigs resulted in a loss of 15 per cent of the information.

3. The slight difference between the results from the random sample and the sample of the four average pigs indicated that there must have been only a small correlation, between weaning weight and subsequent rate of growth. An analysis of post-weaning rate of growth showed that the intra-class correlation of pigs of the same weaning weight was 0·15. Individual weight at weaning would therefore appear to be of slight value in estimating subsequent performance.

4. Intra-litter correlations of growth rate for litter classes eight and nine were found to be 0·3 and 0·5. These values are higher than those found by others, probably as a result of the inclusion of the pre-weaning period in the calculation of the growth rates. There would appear to be grounds for believing that at least a fifth of the individual variance may be accounted for by additive gene effects.

The object of this communication is to give an account of an experiment, carried through in the autumn and winter season of 1934–5, designed to determine the changes in composition involved in making silage by the ordinary, molasses and A.I.V. processes. The changes in composition included those of both crude as well as digestible nutrients.

A short account is given of the main changes which take place during the ensilage process. Details are given of the methods of analysis used in examining samples of silage for pH, crude-protein, volatile base, amino acid, lactic acid, total volatile acid, acetic acid and butyric acid contents. The results of the examination of 293 samples of silage are discussed. Of these 258 were made from grassland herbage, 24 from silage crops, 9 from sugar-beet tops, and 2 from potatoes. The silages were made in towers, wood-lined pits, and a small number in stacks or clamps. The samples are divided up into those made by the ordinary process, with added molasses, with added whey, with moderate amounts of mineral acid with or without molasses, and with mineral acids added in amounts sufficient to bring the mass rapidly to a pH of 3·0–4·0—the A.I.V. process.

For comparative purposes the silages made by these processes have been divided, within each process, into groups of varying pH. reaction.

1. Waksman's simplified scheme of fractionation was used in attempts to trace the organic transformations occurring in some tropical samples of composts, soils and peats.

2. It was found that a useful partition of the nitrogenous substances into hydrolysable and resistant fractions could be effected by boiling with 5 per cent sulphuric acid for 6 hours.

3. Two profile layers of a black calcareous Trinidadian clay soil were compared with two horizons of a prairie soil examined by Waksman & Stevens. The surface layers of both soils were found to be similar in chemical characters, but the lower subsoil layers of the Trinidadian soil gave evidence of a more advanced and profound degree of organic decomposition.

4. Serial soil samples, taken from eight undisturbed natural profiles representative of humid tropical soil types occurring in Jamaica and Trinidad, were similarly examined.

1. It has been shown experimentally that the excretion of nitrogen noted by us in cultures of inoculated legumes takes place from the nodule bacteria, probably from the intranodular ones, and not from the roots. No excretion of amino acids occurs in cultures of uninoculated legumes growing on nitrate nitrogen.

2. Our earlier hypothesis that the legumes receive their nitrogen nutrition from the nodules in the form of organic nitrogen compounds, particularly amino acids, is in perfect accord with our new observations concerning the process of excretion. All facts indicate that the amino acids concerned are primary products of the nitrogen fixation, and not breakdown products of proteins. Bond's valuable work along quite different lines produced results which support this conclusion. He, however, did not study the chemical nature of the nitrogen compounds in question.

3. The excretion of nitrogen occurs in media capable of absorbing the excreted nitrogen compounds (cellulose, kaolin, sand, soil). The demonstration of the excretion is not possible in water cultures except when very large quantities of water are used. On the basis of these facts a hypothesis is advanced to explain the nature of the excretion.

4. The term total fixed nitrogen has been used as an expression for the extent of nitrogen fixation, while the term extent of excretion is employed to indicate that percentage of the total fixed nitrogen which is excreted from the nodules.

5. The extent of excretion depends largely on the strain used for inoculation. With strains of apparently equal effectiveness in nitrogen fixation, the extent of excretion may vary considerably, so that actually such strains differ in their effectiveness.

1. Treatment in bulk of the grain of wheat, barley, winter oats and spring oats with a proprietary dust, and two experimental dusts A and B which contained organic compounds of mercury, had no harmful effect on germination when drilling followed shortly after treatment. In the case of spring oats, drilling was delayed 7½ weeks after treatment without any harmful effects on germination.

2. The proprietary and experimental dusts both proved effective in controlling bunt disease of wheat, and the leaf-stripe and net-blotch diseases of barley.

3. In the case of barley, the proprietary and experimental dusts both increased the speed of “brairding” but not the final plant population. This hastening of seedling growth did not lead to a higher yield.

4. In the case of winter oats, only the experimental dust A gave a significant increase in plant population and a satisfactory control of the loose smut disease.

1. The total yield of dry matter of rye-grass, grown in the presence of inoculated (a) peas, (b) red clover, (c) serradella, in sand with no added nitrogen, after 13 weeks' growth was increased by about three times in the peas-rye-grass series, twice in the clover-rye grass series, and nearly twice in the serradella series in comparison with the yield of rye-grass grown alone.

2. The nitrogen percentage and total nitrogen yield of rye-grass were greatly influenced by associated growth with peas, clover or serradella. Rye grass grown with peas after 13 weeks' growth contained nearly five times, grown with clover three times, grown with serradella about twice, as much total nitrogen, as grass of the same age similarly grown, but in the absence of leguminous plants.

3. Among the three leguminous species tested at Pulawy, peas were the best companions for rye-grass, giving the highest amount of assimilated nitrogen, while serradella gave the smallest.

4. In another experiment, when barley and (a) peas, (b) red clover, (c) lucerne were grown together in sand without added nitrogen, only peas exerted a beneficial influence upon the yield of dry matter and the nitrogen percentage and total nitrogen yield of barley.

The term “guano” is applied almost wholly to the excrement of sea-birds and, since it contains a high proportion of nitrogen and phosphorus, guano forms a very valuable fertilizing material. The Union of South Africa has a large number of small islands scattered round the coast which are used as breeding grounds by various sea-birds. The deposits of guano from different islands vary considerably and are therefore systematically mixed to obtain an approximately uniform grade of material. The average analytical results for the years 1921–8 were: 10·0 per cent nitrogen, 11·2 per cent phosphoric oxide, 2·0 per cent potash.

Composts on which the mushroom mycelium fails to make normal growth are a common cause of poor yields and crop failures. No investigation into the matter appears to have been made, though other aspects of mushroom nutrition have received attention. A good deal of research has been directed towards the discovery of the principal mineral (3, 5, 6, 7, 11) and organic (1, 2, 4, 6, 7, 8, 9) nutrients required by the mushroom. Environmental factors such as temperature (2), partial pressures of gases (12), concentration of soluble materials (6, 7), hydrogen-ion concentration (10, 11) and moisture content (7) of the compost have been studied. But failures occur, of the kind described, when these factors appear to be favourable and when the compost contains ample food material. It has been suggested (l, 2) that poor composts may be unsuitable in physical condition or contain injurious compounds. The influence of the physical or physicochemical condition of the compost on mycelial growth deserves attention, since a compost is a colloidal system composed of solid, liquid and gas phases. In such a system it is possible, without changing the chemical identity of the materials, to bring about great changes in free surface energy, kinetic energy, interfacial tensions, dispersion and hydration of colloids, electrokinetic phenomena, diffusion and osmotic pressures—all of which are probably of vital importance in the nutrition of the mushroom. The properties of stability, swelling and degree of dispersion of most colloidal systems are determined by the nature of the adsorbed ions, especially the adsorbed cations.

1. Experiments were carried out in four seasons at Barberton, South Africa, occupying approximately 2¼ acres of ground. Three soil treatments were used, viz. Normal (N), Compressed (C) and Grubbed (loosened) (G).

2. Measures of consolidation of the soil were made with a specially designed apparatus, and large differences established (pp. 516–520).

3. Estimations of water content and nitrate content were carried out. No great differences were found (pp. 520–524).

4. Fortnightly samples of the plants were taken, from which numerical data were collected designed to estimate morphological development (primary data, pp. 526–529).

5. Derived data on net assimilation rate, relative leaf-growth rate and efficiency index were calculated (pp. 529–533).

6. Growth in total dry weight was found to conform approximately with an exponential law up to the time of flowering. Up to this stage also net assimilation rate was found to remain constant.

7. Growth differences between treatments were related to variation in net assimilation rate only (p. 531).

8. The results were analysed statistically. Significant results alone are emphasized.

9. In all respects growth and development on the consolidated soil was more rapid than that on the grubbed soil, normal being generally intermediate.

10. The results are discussed with reference to the question of nitrogen supply, water supply and aeration of the soil.

In a previous paper(1) reference was made to the subject of seed disinfection, in particular to an investigation on disinfectant dusts containing mercury. It was shown that by the use of certain of these dusts the common seed-borne diseases of cereals (excluding the loose smuts of barley and wheat) could be controlled. The present observations record further work, chiefly greenhouse and field studies, that has been done on green peas. This crop would appear to be of increasing economic importance, since in the past ten years the acreage devoted to it has increased by approximately 49 per cent. Its successful cultivation is often much influenced by climatic and soil conditions, particularly during the first few weeks after sowing. If adverse weather conditions follow, poor germination may result although the seed sown may have been viable and not necessarily diseased. Failures of such a type are usually associated with the rotting of the ungerminated seed in the soil, but from the same bulks, samples sown under more favourable conditions may germinate well and produce a satisfactory crop. Ogilvie (2,3,4) finds that in the Western Advisory province Ascochyta Pisi and Mycosphaerella pinodes are two causes of the early failure of pea plants, and that “pea sickness” is associated with a strain of the eelworm Heterodera schachtii and with foot-rot caused by various species of Fusarium. Premature dying-off of the plants is accompanied sometimes with Heterodera schachtii and foot-rot and sometimes with foot-rot alone.

Some time ago, the author (Hendricks, 1935a) pointed out a modification which may occasionally be desirable in the application of co-variance analysis to experimental data. It was suggested that, in some instances, it is more logical to base the analysis on the assumption that the slope of the line of regression varies from class to class than on the assumption that the regression coefficient has the same value for each class of observations.

1. The effect of low-temperature grain pre-treatment on two winter varieties of wheat and two winter varieties of barley is seen in a very definite reduction in the number of tillers produced, which, however, had no subsequent effect on the number of ears surviving at harvest. Very little effect resulted from the treatment of spring varieties of wheat and barley in the number of tillers produced, although the spring wheat variety did show a slight reduction.

2. Gross yields per plot were unaffected by the treatment in all the varieties, but the yields per plant were affected in the wheat experiment, and the yield per ear in the barley experiment. There was a general increase in the yield per plant of all wheat varieties, while in the yield per ear one barley variety was unaffected, another showed an increase, while the third suffered a reduction.

3. The grain from treated plants was affected in 1000-grain weight and nitrogen content in the wheat experiment, while only the 1000-grain weight suffered any change in the barley experiment. Only one wheat variety showed an increase in 1000-grain weight, while each of the three varieties responded differently with regard to the nitrogen content of the grain. Similarly, each of the three barley varieties was affected differently with regard to 1000-grain weight, one being unaffected, another showing a decrease, while the third showed an increase.

4. Analysis of the behaviour of the varieties with regard to tillering and ear production showed clearly that the maximum of tillers produced bears little relation to the number of ears at harvest. In the barley varieties high yield and low nitrogen were associated with the largest number of ears, which in turn was associated with the lowest tillering. In the wheat varieties high yield was associated with high 1000-grain weight. In both experiments the spring varieties outyielded the winter varieties whether the latter had been treated or not.

1. A preliminary account is given of experiments on the compressibility of soils in field condition, and two methods for obtaining compressibility curves, one for the field and one for the laboratory are described. The laboratory apparatus automatically draws a curve relating deformation to the square root of the load built up.

2. The theoretical relationship between load and deformation is discussed, the conclusions reached being at this stage semi-quantitative.

3. Laboratory compression curves are shown to indicate the characteristics of soils in various states of tilth, and the effects of drainage condition, frost action, etc. are discussed.

4. Such factors as size of soil crumb, depth of layer tested, and moisture content of soil samples for laboratory studies are considered.

5. Preliminary field experiments are described in which the effect of simple cultivation processes on soil compressibility were measured.

6. Tentative conclusions about the significance of the differences in the shape of the laboratory curves are given, though these may need to be modified, and will certainly be extended following further experimentation.

A series of experiments designed to measure the losses in dry matter and nutrients in different types of silage are described. These extend over a period of 4 years. The ordinary or low-temperature process (maximum temperature 80–100° F.) is described either with or without added molasses or whey solutions, and is compared with processes in which solutions of mineral acids are added. The A.I.V. process of Virtanen, in which the acidity of the mass is reduced rapidly to pH. 3·0–4·0 by the addition of a mineral acid solution, is considered in detail.

1. The conditions governing base exchange equilibria in soil profiles are discussed.

2. Exchangeable cation contents of six profiles are reported, the results including figures for Ca, Sr, Mg, Na, K, H and exchange capacity, as well as clay content and pH value.

3. It is shown that cation displacement occurs as a result of the downward movement of hydrogen ions from the surface layers, and a layer of minimal basic cation content exists at about 30 cm. depth.

4. Below this depth the basic cation content increases once again, but the base exchange capacity follows the clay content fairly closely.

5. Calcium and strontium are displaced (and readsorbed) at similar rates, in accordance with their exchange constants, although there is about 400 times as much calcium as strontium present. The behaviour of the other cations also appears to be in accordance with theoretical demands.

The effect of sulphate of ammonia in tending to make soil acid when it is applied as a manure has been well known ever since about 1891 when Wheeler (1) first observed the phenomenon at the Rhode Island Experiment Station. Similar results to those observed by Wheeler have been found all over the world where this manure has been applied for many years on soils containing very little calcium carbonate. One of these cases has occurred at the Woburn Experimental Station, where acidity has developed which has been fatal to continued cultivation of both wheat and barley. Seeing that the soil in the continuous barley experiments at this station has been treated with sulphate of ammonia for fifty years, with or without other mineral manures, the conditions have given an excellent opportunity to study the effect of soils made acid in this way on the growth and composition of the barley plant.

Weights and measurements of sides of bacon made under standard conditions from different breeds and crosses of pigs and exhibited at the London Dairy Show from 1922–31 have been analysed statistically.

1. The actual live weight of the pig affects the carcass percentage more than does breed or type.

2. For a bacon pig of 200 lb. live weight, there is an optimum carcass percentage (about 79 per cent) and an optimum bacon percentage (about 58 per cent). The highest percentage is not necessarily best, for below about this level the proportion of bone will be too large and above this level the proportion of fat will be too large.

3. The reasons why certain measurements and proportions are necessary to meet market requirements are detailed.

4. The changes in the proportions of the side with increase in weight of the side are described. The proportional increase in bacon weight is much greater than the proportional increase in side length. Both belly thickness and thickness of shoulder fat increase in their proportions at a faster rate than side length, but not so fast as side weight. The thickness of fat over the loin is the only part measured which increases at a faster rate than side weight.

5. The subcutaneous layer of fat matures earliest at the shoulder, over the rump next, and on the loin last. The rate of increase of the back fat slows down as the sides increase in weight. This slackening of growth is greatest in the shoulder and least in the loin, the rump area being intermediate.

Feeding trials with sheep, subsisting out-of-doors on typical winter rations of known dry-matter and starch-equivalent content, have been carried out during the winters of 1933–4, 1934–5 and 1935–6. Records have been kept over these periods of the live-weights of the animals and their daily consumption of dry matter and starch equivalent.

It has been found that the standards of appetite, in terms of lb. dry matter, proposed by Prof. T. B. Wood are uniformly too high, a result in harmony with the findings of Prof. J. A. S. Watson and co-workers at Oxford. It is suggested that Prof. Wood's values should be multiplied throughout by the factor 0·85 in order to obtain reasonable measures of the appetites of sheep at different live-weights.

The data from the feeding trials have been used in an attempt to decide between the old and the recently proposed standards for the maintenance starch-equivalent requirement of the 100 lb. sheep, namely, 0·74 and 1·26 lb. of starch equivalent per day. The results point to the reliability of the higher figure, and it is shown that the results of recent work on the energy metabolism of sheep are in harmony with this conclusion.

A table embodying a revision of Prof. Wood's feeding standards for sheep is included in the paper.