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On the diffusion of circular domains on a spherical vesicle

Published online by Cambridge University Press:  11 May 2010

S. ALIASKARISOHI ,
P. TIERNO ,
P. DHAR ,
Z. KHATTARI ,
M. BLASZCZYNSKI  and
TH. M. FISCHER
S. ALIASKARISOHI
Affiliation:
Institut für Experimentalphysik, Universität Bayreuth, 95440 Bayreuth, Germany
P. TIERNO
Affiliation:
Departament de Química Física, Universitat de Barcelona, Marti i Franqes 1, 08028 Barcelona, Spain
P. DHAR
Affiliation:
Department of Chemical Engineering, University of California Santa Barbara, Santa Barbara, CA 93106, USA
Z. KHATTARI
Affiliation:
Department of Physics, Hashemite University, 13115 Zarqa, Jordan
M. BLASZCZYNSKI
Affiliation:
Institut für Experimentalphysik, Universität Bayreuth, 95440 Bayreuth, Germany
TH. M. FISCHER*
Affiliation:
Institut für Experimentalphysik, Universität Bayreuth, 95440 Bayreuth, Germany
*
†Email address for correspondence: thomas.fischer@uni-bayreuth.de
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Abstract

Tracking the motion of lipid domains on a vesicle is a rheological technique allowing the measurement of surface shear viscosities of vesicular lipid phases. The ratio of surface to bulk viscosity defines a viscous length scale. Hydrodynamic interactions split the motion of the domains into different modes of diffusion. The measurability of surface shear viscosities from any mode of diffusion is limited to viscous length scales between the radius of the domains and the radius of the vesicle. The measurability of the surface shear viscosity results from the sensitivity of the diffusion to surface shear viscosities and from sufficient spatial resolution to resolve the diffusive motion. Switching between the various modes of diffusion is a trade between sensitivity gained and resolution lost by the hydrodynamic interactions leaving the measurability unchanged. Measurability drops with the number of domains making single-domain rheology the best technique to measure surface shear viscosities. Ultimately confinement of the domains to small vesicles renders measurements of surface rheological properties with domain-tracking rheology impossible. Experiments on domains in vesicles of a mixture of dioleoylphosphatidylcholine (DOPC), dipalmytoylphosphatidylcholin (DPPC) and cholesterol (Chol) exhibit diffusion that is entirely controlled by dissipation into the water. The diffusion is suppressed compared to the diffusion of isolated domains in a flat membrane due to confinement to the curved vesicle and by hydrodynamic interactions between the domains. Effects of surface shear viscosity can be neglected.

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Type
Papers
Information
Journal of Fluid Mechanics , Volume 654 , 10 July 2010 , pp. 417 - 451
Copyright
Copyright © Cambridge University Press 2010

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