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Design and synthesis of an antigenic mimic of the Ebola glycoprotein

Published online by Cambridge University Press:  31 January 2011

Ryan D. Rutledge ,
Brian J. Huffman ,
David E. Cliffel  and
David W. Wright
Ryan D. Rutledge
Affiliation:
Vanderbilt University, Department of Chemistry, Nashville, Tennessee 37235
Brian J. Huffman
Affiliation:
Vanderbilt University, Department of Chemistry, Nashville, Tennessee 37235
David E. Cliffel
Affiliation:
Vanderbilt University, Department of Chemistry, Nashville, Tennessee 37235
David W. Wright*
Affiliation:
Vanderbilt University, Department of Chemistry, Nashville, Tennessee 37235
*
a)Address all correspondence to this author. e-mail: david.wright@vanderbilt.edu
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Abstract

An antigenic mimic of the Ebola glycoprotein was synthesized and tested for its ability to be recognized by an anti-Ebola glycoprotein antibody. Epitope-mapping procedures yielded a suitable epitope that, when presented on the surface of a nanoparticle, forms a structure that is recognized by an antibody specific for the native protein. This mimic-antibody interaction has been quantitated through ELISA and QCM-based methods and yielded an affinity (Kd = 12 × 10−6 M) within two orders of magnitude of the reported affinity of the native Ebola glycoprotein for the same antibody. These results suggest that the rational design approach described herein is a suitable method for the further development of protein-based antigenic mimics with potential applications in vaccine development and sensor technology.

Keywords

AuBiomimetic (assembly)Nanostructure

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Type
Articles
Information
Journal of Materials Research , Volume 23 , Issue 12: Biomimetic and Bio-enabled Materials Science and Engineering , December 2008 , pp. 3161 - 3168
Copyright
Copyright © Materials Research Society 2008

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