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Osteogenic differentiation of mesenchymal stem cells on hybrid coatings sterilized by different processes

Published online by Cambridge University Press:  14 October 2019

Estela K. Kerstner Baldin
Affiliation:
LAPEC—Research Laboratory Corrosion, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS 91501-970, Brazil
Célia de Fraga Malfatti
Affiliation:
LAPEC—Research Laboratory Corrosion, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS 91501-970, Brazil
Rosmary Nichele Brandalise
Affiliation:
PGPROTEC—Postgraduate Program in Process and Technology Engineering, University of Caxias do Sul (UCS), Caxias do Sul, RS 95070-560, Brazil
Bruno Meira Soares
Affiliation:
LACOM—Laboratory of Analysis of Organic Compounds and Metals, Federal University of Rio Grande (FURG), Rio Grande, RS 96203-900, Brazil
Daniela Pavulack
Affiliation:
IPCT—Institute for Research on Stem Cells, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS 90610-000, Brazil
Daniela Steffens
Affiliation:
Postgraduate Program in Pathology, Federal University of Health Sciences of Porto Alegre (UFCSPA), Porto Alegre, RS 90050-170, Brazil
Patricia Pranke
Affiliation:
Hematology and Stem Cell Laboratory, Faculty of Pharmacy, and Post Graduate Program in Physiology, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS 90610-000, Brazil
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Abstract

The objective of the present work was to evaluate the behavior of osteogenesis of mesenchymal stem cells (MSCs) on a double-layer, protective, and bioactive hybrid coating sterilized by 3 different processes: steam autoclave, hydrogen peroxide plasma, and ethylene oxide. The hybrid coating was obtained from a sol consisting of the silane precursors tetraethoxysilane (TEOS) and methyltriethoxysilane (MTES), applied on a Ti6Al4V substrate. To promote bioactivity, hydroxyapatite (HA) particles were dispersed in a second coating (bioactive layer: TEOS/MTES + HA) applied on the first (TEOS/MTES). The sterilized coatings were evaluated by scanning electron microscopy, wettability, and micrometer roughness. The behavior of hydrolytic degradation was evaluated by the mass variation of the samples and the release of silicon by the technique of high-resolution atomic absorption spectrometry. All coatings presented morphological and superficial alterations after sterilization. Sterilization by ethylene oxide and hydrogen peroxide plasma intensified the hydrolytic degradation of the bioactive coating causing a greater release of silicon. The sterilized hybrid coatings did not show cytotoxicity to MSCs. Adhesion, viability, and osteogenic differentiation were favored on the sterilized coating of hydrogen peroxide plasma, which is opposite to what was observed for the ethylene oxide-sterilized coating.

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Copyright © Materials Research Society 2019 

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