Hostname: page-component-848d4c4894-jbqgn Total loading time: 0 Render date: 2024-06-17T08:34:20.787Z Has data issue: false hasContentIssue false

Silicon carbide quantum dots for bioimaging

Published online by Cambridge University Press:  28 September 2012

David Beke*
Affiliation:
Institute for Solid State Physics and Optics, Wigner Research Centre for Physics, Hungarian Academy of Sciences, H-1121 Budapest, Hungary; and Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1111 Budapest, Hungary
Zsolt Szekrényes
Affiliation:
Institute for Solid State Physics and Optics, Wigner Research Centre for Physics, Hungarian Academy of Sciences, H-1121 Budapest, Hungary
Denes Pálfi
Affiliation:
The Faculty of Information Technology, Pázmány Péter Catholic University, H-1083 Budapest, Hungary; and Institute of Experimental Medicine, Hungarian Academy of Sciences, H-1083 Budapest, Hungary
Gergely Róna
Affiliation:
Institute of Enzymology, Research Centre of Natural Sciences, Hungarian Academy of Sciences, H-1113 Budapest, Hungary
István Balogh
Affiliation:
Institute for Solid State Physics and Optics, Wigner Research Centre for Physics, Hungarian Academy of Sciences, H-1121 Budapest, Hungary
Pal Andor Maák
Affiliation:
Department of Atomic Physics, Budapest University of Technology and Economics, H-1111 Budapest, Hungary
Gergely Katona
Affiliation:
Institute of Experimental Medicine, Hungarian Academy of Sciences, H-1083 Budapest, Hungary
Zsolt Czigány
Affiliation:
Institute for Technical Physics and Materials Science, Research Centre of Natural Sciences, Hungarian Academy of Sciences, H-1121 Budapest, Hungary
Katalin Kamarás
Affiliation:
Institute for Solid State Physics and Optics, Wigner Research Centre for Physics, Hungarian Academy of Sciences, H-1121 Budapest, Hungary
Balazs Rózsa
Affiliation:
The Faculty of Information Technology, Pázmány Péter Catholic University, H-1083 Budapest, Hungary; and Institute of Experimental Medicine, Hungarian Academy of Sciences, H-1083 Budapest, Hungary
Laszlo Buday
Affiliation:
Institute of Enzymology, Research Centre of Natural Sciences, Hungarian Academy of Sciences, H-1113 Budapest, Hungary
Beata Vértessy
Affiliation:
Institute of Enzymology, Research Centre of Natural Sciences, Hungarian Academy of Sciences, H-1113 Budapest, Hungary
Adam Gali
Affiliation:
Institute for Solid State Physics and Optics, Wigner Research Centre for Physics, Hungarian Academy of Sciences, H-1121 Budapest, Hungary; and Department of Atomic Physics, Budapest University of Technology and Economics, H-1111 Budapest, Hungary
*
a)Address all correspondence to this author. e-mail: beke.david@wigner.mta.hu
Get access

Abstract

Luminescent nanocrystals or quantum dots (QDs) have great potential for bioanalysis as well as optoelectronics. Here we report an effective and inexpensive fabrication method of silicon carbide quantum dots (SiC QDs), with diameter below 8 nm, based on electroless wet chemical etching. Our samples show strong violet-blue emission in the 410–450 nm region depending on the solvents used and particle size. The cytotoxic properties of the SiC QDs based on alamarBlueTM assay cells were studied. The presence of the QDs dots does not affect cell growth in a wide concentration range. Two-photon excitation showed significant response from SiC nanocrystals that were injected into hippocampal CA1 pyramidal cells.

Type
Articles
Copyright
Copyright © Materials Research Society 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Murcia, M.J. and Naumann, C.A.: Biofunctionalization of fluorescent nanoparticles, in Nanotechnologies for Life Sciences, Vol. 1, edited by Kumar, S.S.R. (Wiley-VCH, Weinheim, 2005), pp. 140.Google Scholar
Medintz, I.L., Uyeda, H.T., Goldman, E.R., and Mattoussi, H.: Quantum dot bioconjugates for imaging, labeling and sensing. Nat. Mater. 4, 435, (2005).CrossRefGoogle ScholarPubMed
Kim, S., Fisher, B., Eisler, H.J., and Bawendi, M.: Type-II quantum dots: CdTe/CdSe (core/shell) and CdSe/ZnTe (core/shell) heterostructures. J. Am. Chem. Soc. 125, 11466, (2003).CrossRefGoogle ScholarPubMed
Cao, Y.W., Aksenton, J., Soloviev, V., and Banin, U.: Colloidal synthesis and properties of InAs/InP and InAs/CdSe core/shell, nanocrystals. in Semiconductor Quantum Dots, edited by Moss, S.C., Ila, D., Lee, H.W.H., and Norris, D.J. (Mater. Res. Soc. Symp. Proc. 571, Warrendale, PA, 2000) p. 75.Google Scholar
Hardman, R.: A toxicologic review of quantum dots: Toxicity depends on physicochemical and environmental factors. Environ. Health Perspect. 114, 165, (2006).CrossRefGoogle ScholarPubMed
Hua, F., Erogbogbo, F., Yong, K.T., Roy, I., Xu, G.X., Prasad, P.N., and Swihart, M.T.: Organically capped silicon nanoparticles with blue photoluminescence prepared by hydrosilylation followed by oxidation. ACS Nano 2, 873, (2008).Google Scholar
Kanemitsu, Y., Shimizu, N., Komoda, T., Hemment, P.L.F., and Sealy, B.J.: Photoluminescent spectrum and dynamics of Si+-ion-implanted and thermally annealed SiO2 glasses. Phys. Rev. B 54, R14329 (1996).CrossRefGoogle Scholar
Hadjisawas, G. and Kelires, P.: Structure and energetics of Si nanocrystals embedded in a-SiO2. Phys. Rev. Lett. 93, 226104 (2004).CrossRefGoogle Scholar
Wu, X., Fan, J., Qiu, T., Yang, X., Siu, G., and Chu, P.K.: Experimental evidence for the quantum confinement effect in 3C-SiC nanocrystallites. Phys. Rev. Lett. 94, 6 (2005).CrossRefGoogle ScholarPubMed
Fan, J.Y., Wu, X.L., Li, H.X., Liu, H.W., Siu, G.G., and Chu, P.K.: Luminescence from colloidal 3C-SiC nanocrystals in different solvents. Appl. Phys. Lett. 88, 041909 (2006).CrossRefGoogle Scholar
Botsoa, J., Bluet, J.M., Lysenko, V., Marty, O., Barbier, D., and Guillot, G.: Photoluminescence of 6H–SiC nanostructures fabricated by electrochemical etching. J. Appl. Phys. 102, 083526 (2007).CrossRefGoogle Scholar
Makkai, Z., Pécz, B., Bársony, I., Vida, G., Pongrácz, A., Josepovits, K.V., and Deák, P.: Isolated SiC nanocrystals in SiO2. Appl. Phys. Lett. 86, 253109 (2005).CrossRefGoogle Scholar
Coletti, C., Jaroszeski, M.J., Pallaoro, A., Hoff, A.M., Iannotta, S., and Saddow, S.E.: Biocompatibility and wettability of crystalline SiC and Si surfaces. In IEEE EMBS Proceedings 29th Annual International Conference. 58495852 (EMBS, Lyon, France, 2007).Google Scholar
Raya, C.T., Maldonado, D.H., Rico, J.R., Gañan, C.G., de Arellano-Lopez, A.R., and Fernandez, J.M.: Fabrication, chemical etching, and compressive strength of porous biomimetic SiC for medical implants. J. Mater. Res. 23, 32473254 (2008).CrossRefGoogle Scholar
Botsoa, J., Lysenko, V., Géloën, A., Marty, O., Bluet, J.M., and Guillot, G.: Application of 3C-SiC quantum dots for living cell imaging. Appl. Phys. Lett. 92, 173902 (2008).CrossRefGoogle Scholar
Serdiuk, T., Lysenko, V., Skryshevsky, V., and Géloën, A.: Vapor phase-mediated cellular uptake of sub-5 nm nanoparticles. Nanoscale Res. Lett. 7, 212 (2012).CrossRefGoogle ScholarPubMed
Beke, D., Szekrényes, Zs., Balogh, I., Veres, M., Fazakas, É., Varga, L.K., Kamarás, K., Czigány, Zs., and Gali, A.: Characterization of luminescent silicon carbide nanocrystals prepared by reactive bonding and subsequent wet chemical etching. Appl. Phys. Lett. 99, 213108 (2011).CrossRefGoogle Scholar
Zhu, J., Liu, Z., Wu, X.L., Xu, L.L., Zhang, W.C., and Chu, P.K.: Luminescent small-diameter 3C-SiC nanocrystals fabricated via a simple chemical etching method. Nanotechnology 18, 365603 (2007).CrossRefGoogle Scholar
Katona, G., Szalay, G., Maák, P., Kaszás, A., Veress, M., Hillier, D., Chiovini, B., Vizi, E.S., Roska, B., and Rózsa, B.: Fast two-photon in vivo imaging with three-dimensional random-access scanning in large tissue volumes. Nat. Methods 9, 201 (2012).CrossRefGoogle ScholarPubMed
Maravall, M., Mainen, Z.F., Sabatini, B.L., and Svoboda, K.: Estimating intracellular calcium concentrations and buffering without wavelength ratioing. Biophys. J. 78, 26552667 (2000).CrossRefGoogle ScholarPubMed