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Study on cytotoxicity of polyethylene glycol and albumin bovine serum molecule–modified quantum dots prepared by hydrothermal method

Published online by Cambridge University Press:  17 April 2020

Enlv Hong ,
Lumin Liu ,
Chen Li ,
Dan Shan ,
Hailong Cao  and
Baiqi Wang
Enlv Hong
Affiliation:
Department of Occupational and Environmental Health, School of Public Health, Tianjin Medical University, Tianjin 300070, China
Lumin Liu
Affiliation:
Department of Occupational and Environmental Health, School of Public Health, Tianjin Medical University, Tianjin 300070, China
Chen Li
Affiliation:
Department of Occupational and Environmental Health, School of Public Health, Tianjin Medical University, Tianjin 300070, China
Dan Shan
Affiliation:
Department of Occupational and Environmental Health, School of Public Health, Tianjin Medical University, Tianjin 300070, China
Hailong Cao*
Affiliation:
Department of Gastroenterology and Hepatology, General Hospital, Tianjin Medical University, Tianjin 300052, China
Baiqi Wang*
Affiliation:
Department of Occupational and Environmental Health, School of Public Health, Tianjin Medical University, Tianjin 300070, China Tianjin Institute of Digestive Disease, Tianjin Key Laboratory of Environment, Nutrition and Public Health, Tianjin 300070, China National Demonstration Center for Experimental Preventive, Medicine Education (Tianjin Medical University), Tianjin 300070, China
*
*)Address all correspondence to these authors. e-mail: Cao HL: caohailong@tmu.edu.cn and Wang BQ: wbqpaper@126.com
*)Address all correspondence to these authors. e-mail: Cao HL: caohailong@tmu.edu.cn and Wang BQ: wbqpaper@126.com
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Abstract

Fluorescent quantum dots (QDs) modified with polyethylene glycol (PEG) and albumin bovine serum (BSA) have profound application in the detection and treatment of hepatocellular carcinoma (HCC) cells. In the present study, the effects and mechanism of PEG and BSA modification on the cytotoxicity of QDs have been explored. It was found that the diameter of the as-prepared QDs, PEG@QDs, BSA@QDs is 3–5 nm, 4–5 nm, and 4–6 nm, respectively. With increase of the treatment time from 0 to 24 h, the HCC cell viability treated with QDs, PEG@QDs, and BSA@QDs obviously decreases, showing a certain time-dependent manner. When the concentration of several nanomaterials is increased from 10 to 90 nM, the cell viability decreases accordingly, exhibiting a certain concentration-dependent manner. Under the same concentration change conditions, the reactive oxygen species contents of cells treated by QDs, PEG@QDs, and BSA@QDs also rise from 7.9 × 103, 6.7 × 103, and 4.7 × 103 to 13.2 × 103, 14.3 × 103, and 12.3 × 103, respectively. In these processes, superoxide dismutase does not play a major role. This study provides strong foundation and useful guidance for QD applications in the diagnosis and treatment of HCC.

Keywords

quantum dotssurface modificationhepatocellular carcinomascell activityreactive oxygen species
Type
Article
Information
Journal of Materials Research , Volume 35 , Issue 9 , 14 May 2020 , pp. 1135 - 1142
Copyright
Copyright © Materials Research Society 2020

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Footnotes

#)

These authors contributed equally to this work.

References

Siegel, R.L., Miller, K.D., and Jemal, A.: Cancer statistics, 2020. Ca-Cancer J. Clin. 70, 7–30 (2020).CrossRefGoogle ScholarPubMed
Bosch, F.X., Ribes, J., Diaz, M., and Cleries, R.: Primary liver cancer: Worldwide incidence and trends. Gastroenterology 127, S5–S16 (2004).CrossRefGoogle ScholarPubMed
Survival Rates for Liver Cancer, American Cancer Society: Available at: https://www.cancer.org/cancer/liver-cancer/detection-diagnosis-staging/survival-rates.html (accessed March 2, 2020).Google Scholar
Ferrari, M.: Cancer nanotechnology: Opportunities and challenges. Nat. Rev. Cancer 5, 161–171 (2005).CrossRefGoogle ScholarPubMed
Bertrand, N., Wu, J., Xu, X.Y., Kamaly, N., and Farokhzad, O.C.: Cancer nanotechnology: The impact of passive and active targeting in the era of modern cancer biology. Adv. Drug Delivery Rev. 66, 2–25 (2014).CrossRefGoogle ScholarPubMed
Juzenas, P., Chem, W., Sun, Y.P., Coelho, M.A.N., Generalov, R., Generalova, N., and Christensen, I.L.: Intracellular delivery: Fundamentals and applications. Adv. Drug Delivery Rev. 60, 1600–1614 (2008).CrossRefGoogle Scholar
Biju, V., Mundayoor, S., Omkumar, R.V., Anas, A., and Ishikawa, M.: Bioconjugated quantum dots for cancer research: Present status, prospects and remaining issues. Biotechnol. Adv. 28, 199 (2010).CrossRefGoogle ScholarPubMed
Yong, K.T., Wang, Y.C., Roy, I., Rui, H., Swihart, M.T., Law, W.C., Kwak, S.K., Ye, L., Liu, J.W., Mahajan, S.D., and Reynolds, J.L.: Preparation of quantum dot/drug nanoparticle formulations for traceable targeted delivery and therapy. Theranostics 2, 681–694 (2012).CrossRefGoogle ScholarPubMed
Biju, V., Itoh, T., Anas, A., Sujith, A., and Ishikaw, M.: Semiconductor quantum dots and metal nanoparticles: Syntheses, optical properties, and biological applications. Anal. Bioanal. Chem. 391, 2469–2495 (2008).CrossRefGoogle ScholarPubMed
Michalet, X., Pinaud, F.F., Bentolila, L.A., Tsay, J.M., Doose, S., Li, J.J., Sundaresan, G., Wu, A.M., Gambhir, S.S., and Weiss, S.: Quantum dots for live cells, in vivo imaging, and diagnostics. Science 307, 538–544 (2005).CrossRefGoogle ScholarPubMed
Zhao, D., He, Z.K., Chan, W.H., and Choi, M.M.F.: Synthesis and characterization of high-quality water-soluble near-infrared-emitting CdTe/CdS quantum dots capped by N-acetyl-L-cysteine via hydrothermal method. J. Phys. Chem. C 113, 1293–1300 (2009).CrossRefGoogle Scholar
Zhang, H., Wang, L.P., Xiong, H.M., Hu, L.H., Yang, B., and Li, W.: Hydrothermal synthesis for high-quality CdTe nanocrystals. Adv. Mater. 15, 1712–1715 (2003).CrossRefGoogle Scholar
Meng, H., Chen, J.Y., Mi, L., Wang, P.N., Ge, M.Y., Yue, Y., and Dai, N.: Conjugates of folic acids with BSA-coated quantum dots for cancer cell targeting and imaging by single-photon and two-photon excitation. J. Biol. Inorg Chem. 16, 117–123 (2011).CrossRefGoogle ScholarPubMed
Li, K., Xia, C., Wang, B., Chen, H., Wang, T., He, Q., Cao, L., and Wang, Y.: Effects of quantum dots on the ROS amount of liver cancer stem cells. Colloids Surf., B 155, 193–199 (2017).CrossRefGoogle ScholarPubMed
Upadhyay, Y., Bothra, S., Kumar, R., Ashok Kumar, S.K., and Sahoo, S.K.: Mimicking biological process to detect alkaline phosphatase activity using the vitamin B6 cofactor conjugated bovine serum albumin capped CdS quantum dots. Colloids Surf., B 185, 110624 (2020).CrossRefGoogle ScholarPubMed
Liu, J., Li, C., Brans, T., Harizaj, A., Steene, S.V., Beer, T.D., Smedt, S.D., Szunerits, S., Boukherroub, R., Xiong, R., and Braeckmans, K.: Surface functionalization with polyethylene glycol and polyethyleneimine improves the performance of graphene-based materials for safe and efficient intracellular delivery by laser-induced photoporation. Int. J. Mol. Sci. 21, 1540 (2020).CrossRefGoogle ScholarPubMed
Liu, L., Jiang, H., Dong, J., Zhang, W., Dang, G., Yang, M., Li, Y., Chen, H., Ji, H., and Dong, L.: PEGylated MoS2 quantum dots for traceable and pH-responsive chemotherapeutic drug delivery. Colloids Surf., B 185, 110590 (2020).CrossRefGoogle ScholarPubMed
Ko, N.R., Van, S.Y., Hong, S.H., Kim, S., Kim, M., Lee, J.S., Lee, S.J., Lee, Y., Kwon, I.K., and Oh, S.J.: Dual pH- and GSH-responsive degradable PEGylated graphene quantum dot-based nanoparticles for enhanced HER2-positive breast cancer therapy. Nanomaterials 10, 91 (2020).CrossRefGoogle ScholarPubMed
Wiseman, H. and Halliwell, B.: Damage to DNA by reactive oxygen and nitrogen species: Role in inflammatory disease and progression to cancer. Biochem. J. 313, 17–29 (1996).CrossRefGoogle ScholarPubMed
Schumacker, P.T.: Reactive oxygen species in cancer cells: Live by the sword, die by the sword. Cancer Cell 10, 175–176 (2006).CrossRefGoogle ScholarPubMed
Loo, D.T. and Rillerna, J.R.: Measurement of cell death. Methods Cell Biol. 57, 251–264 (1998).CrossRefGoogle ScholarPubMed
Thannickal, V.J. and Fanburg, B.L.: Reactive oxygen species in cell signaling. Am J Physiol-Lung C 279, L1005–L1028 (2000).CrossRefGoogle ScholarPubMed
Sauer, H., Watenberg, M., and Hescheler, J.: Reactive oxygen species as intracellular messengers during cell growth and differentiation. Cell. Physiol. Biochem. 11, 173–186 (2001).CrossRefGoogle ScholarPubMed
Hensley, K., Robinson, K.A., Gabbita, S.P., Salsman, S., and Floyd, R.A.: Reactive oxygen species, cell signaling, and cell injury. Free Radicals Biol. Med. 28, 1456–1462 (2000).CrossRefGoogle ScholarPubMed
Li, K.G., Chen, J.T., Bai, S.S., Wen, X., Song, S.Y., Yu, Q., Li, J., and Wang, Y.Q.: Intracellular oxidative stress and cadmium ions release induce cytotoxicity of unmodified cadmium sulfide quantum dots. Toxicol. Vitro 23, 1007–1013 (2009).CrossRefGoogle ScholarPubMed
Wang, J., Zheng, X., and Zhang, H.: Exploring the conformational changes in fibrinogen by forming protein corona with CdTe quantum dots and the related cytotoxicity. Spectrochim. Acta 220, 117143 (2019).CrossRefGoogle ScholarPubMed
Monaheng, N.M., Parani, S., Gulumian, M., and Oluwafemi, O.S.: Eco-friendly synthesis of glutathione-capped CdTe/CdSe/ZnSe core/double shell quantum dots: Its cytotoxicity and genotoxicity effects on Chinese hamster ovary cells. Toxicol. Res. 8, 868–874 (2019).CrossRefGoogle ScholarPubMed
Valko, M., Rhodes, C.J., Moncol, J., Izakovic, M., and Mazur, M.: Free radicals, metals, and antioxidants in oxidative stress-induced cancer. Chem.-Biol. Interact. 160, 1–40 (2006).CrossRefGoogle ScholarPubMed
Blokhina, O., Virolainen, E., and Fagerstedt, K.V.: Antioxidants, oxidative damage and oxygen deprivation stress: A review. Ann. Bot. 91, 179–194 (2003).CrossRefGoogle ScholarPubMed
Inoue, M., Sato, E.F., Nishikawa, M., Park, A.M., Kira, Y., Imada, I., and Utsumi, K.: Cross talk of nitric oxide, oxygen radicals, and superoxide dismutase regulates the energy metabolism and cell death and determines the fates of aerobic life. Antioxid. Redox Signaling 5, 475–484 (2003).CrossRefGoogle ScholarPubMed
Buttner, G.R., Ng, C.F., Wang, M., Rodgers, V.G.J., and Schafer, F.Q.: A new paradigm: Manganese superoxide dismutase influences the production of H2O2 in cells and thereby their biological state. Free Radicals Biol. Med. 41, 1338–1350 (2006).CrossRefGoogle Scholar
Chang, B., Yang, X., Wang, F., Wang, Y., Yang, R., Zhang, N., and Wang, B.: Water soluble fluorescence quantum dot probe labeling liver cancer cells. J. Mater. Sci.: Mater. Med. 24, 2505–2508 (2013).Google ScholarPubMed
Wang, B., Chen, H., Yang, R., Wang, F., Zhou, P., and Zhang, N.: Highly fluorescent QD probes labeling hepatocellular carcinoma cells. RSC Adv. 5, 1841 (2015).CrossRefGoogle Scholar
Gopee, N.V., Roberts, D.W., Webb, P., Cozart, C.R., Siitonen, P.H., Warbritton, A.R., Yu, W.W., Colvin, V.L., Walker, N.J., and Howard, P.C.: Migration of intradermally injected quantum dots to sentinel organs in mice. Toxicol. Sci. 98, 249–257 (2007).CrossRefGoogle ScholarPubMed
Li, M.Y., Ge, Y.X., Chen, Q.F., Xu, S.K., Wang, N.Z., and Zhang, X.J.: Hydrothermal synthesis of highly luminescent CdTe quantum dots by adjusting precursors’ concentration and their conjunction with BSA as biological fluorescent probes. Talanta 72, 89–94 (2007).CrossRefGoogle ScholarPubMed