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Biomimetic calcium carbonate–gelatin composites as a model system for eggshell mineralization

Published online by Cambridge University Press:  27 November 2012

Oliver E. Armitage ,
Daniel G.T. Strange  and
Michelle L. Oyen
Oliver E. Armitage
Affiliation:
Department of Engineering, Cambridge University, Cambridge, CB2 1PZ, United Kingdom
Daniel G.T. Strange
Affiliation:
Department of Engineering, Cambridge University, Cambridge, CB2 1PZ, United Kingdom
Michelle L. Oyen*
Affiliation:
Department of Engineering, Cambridge University, Cambridge, CB2 1PZ, United Kingdom
*
a)Address all correspondence to this author. e-mail: mlo29@eng.cam.ac.uk
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Abstract

The composite nature of mineralized natural materials is achieved through both the microstructural inclusion of an organic component and an overall microstructure that is controlled by templating onto organic macromolecules. A modification of an existing laboratory technique is developed for the codeposition of a CaCO3–gelatin composite with a controllable organic content. First, calibration curves are developed to determine the organic content of a CaCO3–gelatin composite from infrared spectra. Second, a CaCO3–gelatin composite is deposited on either glass coverslips or demineralized eggshell membranes using an automated alternating soaking process. Electron microscopy images and use of the infrared spectra calibration curves show that by altering the amount of gelatin in the ionic growth solutions, the final organic component of the mineral can be regulated over the range of 1–10%, similar to that of natural eggshell.

Keywords

biomimetic (assembly)compositecrystal growth
Type
Articles
Information
Journal of Materials Research , Volume 27 , Issue 24 , 28 December 2012 , pp. 3157 - 3164
Copyright
Copyright © Materials Research Society 2012

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