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Chitosan/hollow silica sphere nanocomposites for wound healing application

  • Ayşe Aslan (a1), Silviya Elanthikkal (a2) and Ayhan Bozkurt (a3)
Abstract

Chitosan is an important cationic biopolymer widely used in various biomedical applications such as wound care, drug delivery, biomaterial scaffolds, and tissue engineering. In this work, hollow silica sphere (HSS) nanoparticles with an average size of 400–450 nm (via TEM) were synthesized by sol–gel process and were epoxidized using epichlorohydrin. Then, chitosan was chemically bound to the epoxidized HSS to form a bionanocomposite, HSS-epoxy-CHI-X where X represents the percentage of chitosan in the sample. BET analysis showed that pure HSS has a specific surface area (Ass) of around 4.8 m2/g and adsorption average pore diameter of 10.18 nm. Bionanocomposites were characterized by FT-IR spectroscopy to confirm the bonding of chitosan on HSS nanoparticles. X-ray diffraction studies demonstrated that the amorphous character of the materials improved with the HSS content. Thermogravimetric analysis illustrated that at low temperatures, the thermal stability of bionanocomposites was higher than that of the sample with no chitosan in it. Scanning electron microscopic analysis results confirmed the homogenous distribution of HSS in the bionanocomposites. Considering the biological activity of chitosan and absorption characteristics of HSS, newly developed HSS-epoxy-CHI-X bionanocomposites were tested for wound healing by in vitro scratch assay using NIH 3T3 fibroblast cells. Among the bionanocomposites analyzed, HSS-epoxy-CHI-60 exhibited better performance where about 90% wound closure was observed for this sample after 21 h of exposure.

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a)Address all correspondence to this author. e-mail: abozkurt@iau.edu.sa
References
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1.Muzzarelli, R.A.A.: Chitins and chitosans for the repair of wounded skin, nerve, cartilage and bone. Carbohydr. Polym. 76, 167 (2009).
2.Ahmed, S., Jayachandran, M., Qureshi, M.A., and Ikram, S.M.: Chitosan based dressings for wound care. Immunochem. Immunopathol. 1, 1 (2015).
3.Ahmed, S. and Ikram, S.: Chitosan based scaffolds and their applications in wound healing. Achiev. Life Sci. 10, 27 (2016).
4.Zhou, Y., Yang, D., Chen, X., Xu, Q., Lu, F., and Nie, J.: Electrospun water-soluble carboxyethyl chitosan/poly(vinyl alcohol) nanofibrous membrane as potential wound dressing for skin regeneration. Biomacromolecules 9, 349 (2008).
5.Zhou, Y., Yang, H., Liu, X., Mao, J., Gu, S., and Xu, W.: Electrospinning of carboxyethyl chitosan/poly(vinyl alcohol)/silk fibroin nanoparticles for wound dressings. Int. J. Biol. Macromol. 53, 88 (2013).
6.Nguyen, V.C., Nguyen, V.B., and Hsieh, M.F.: Curcumin-loaded chitosan/gelatin composite sponge for wound healing application. Int. J. Polym. Sci. 2013, 1 (2013).
7.Ribeiro, M.P., Espiga, A., Silva, D., Baptista, P., Henriques, J., Ferreira, C., Silva, J.C., Borges, J.P., Pires, E., Chaves, P., and Correia, I.J.: Development of a new chitosan hydrogel for wound dressing. Wound Repair Regen. 17, 817 (2009).
8.Zhang, X., Yang, D., and Nie, J.: Chitosan/polyethylene glycol diacrylate films as potential wound dressing material. Int. J. Biol. Macromol. 43, 456 (2008).
9.Deng, Z., Zhen, Z., Hu, X., Wu, S., Xu, Z., and Chu, P.K.: Hollow chitosan-silica nanospheres as pH-sensitive targeted delivery carriers in breast cancer therapy. Biomaterials 32, 4976 (2011).
10.Liberman, A., Mendez, N., Trogler, W.C., and Kummel, A.C.: Synthesis and surface functionalization of silica nanoparticles for nanomedicine. Surf. Sci. Rep. 69, 132 (2014).
11.Gui, R., Wang, Y., and Sun, J.: Embedding fluorescent mesoporous silica nanoparticles into biocompatible nanogels for tumor cell imaging and thermo/pH-sensitive in vitro drug release. Colloids Surf., B 116, 518 (2014).
12.Liu, W.T., Yang, Y., Shen, P.H., Gao, X.J., He, S.Q., Liu, H., and Zhu, C.S.: Facile and simple preparation of pH-sensitive chitosan-mesoporous silica nanoparticles for future breast cancer treatment. Express Polym. Lett. 9, 1068 (2015).
13.Jiao, J., Li, X., Zhang, S., Liu, J., Di, D., Zhang, Y., Zhao, Q., and Wang, S.: Redox and pH dual-responsive PEG and chitosan-conjugated hollow mesoporous silica for controlled drug release. Mater. Sci. Eng., C 67, 26 (2016).
14.Bao, Y., Shi, C., Wang, T., Li, X., and Ma, J.: Recent progress in hollow silica: Template synthesis, morphologies and applications. Microporous Mesoporous Mater. 227, 121 (2016).
15.Chen, A., Yu, Y., Lv, H., Zhang, Y., Xing, T., and Yu, Y.: Synthesis of hollow mesoporous silica spheres and carambola-like silica materials with a novel resin sphere as template. Mater. Lett. 135, 43 (2014).
16.Dong, Y., Wang, E., Yu, L., Wang, R., Zhu, Y., Fu, Y., and Ni, Q.Q.: Self-templated route to synthesis bowl-like and deflated balloon-like hollow silica spheres. Mater. Lett. 206, 150 (2017).
17.Hah, H.J., Kim, J.S., Jeon, B.J., Koo, S.M., and Lee, Y.E.: Simple preparation of monodisperse hollow silica particles without using templates. Chem. Commun. 14, 1712 (2003).
18.Wang, Q., Liu, Y., and Yan, H.: Mechanism of a self-templating synthesis of monodispersed hollow silica nanospheres with tunable size and shell thickness. Chem. Commun. 23, 2339 (2007).
19.Aslan, A., Soydan, A.M., and Bozkurt, A.: Synthesis and characterization of novel multifunctional polymer grafted hollow silica spheres. J. Mater. Res. 30, 2408 (2015).
20.Chen, F. and Zhu, Y.: Chitosan enclosed mesoporous silica nanoparticles as drug nano-carriers: Sensitive response to the narrow pH range. Microporous Mesoporous Mater. 150, 83 (2012).
21.Sun, X., Tang, Z., Pan, M., Wang, Z., Yang, H., and Liu, H.: Chitosan/kaolin composite porous microspheres with high hemostatic efficacy. Carbohydr. Polym. 177, 135 (2017).
22.Wang, Y., Fu, Y., Li, J., Mu, Y., Zhang, X., Zhang, K., Liang, M., Feng, C., and Chen, X.: Multifunctional chitosan/dopamine/diatom-biosilica composite beads for rapid blood coagulation. Carbohydr. Polym. 200, 6 (2018).
23.Alzina, C., Sbirrazzuoli, N., and Mija, A.: Epoxy amine based nanocomposites reinforced by silica nanoparticles. Relationships between morphologic aspects, cure kinetics, and thermal properties. J. Phys. Chem. C. 115, 22789 (2011).
24.Xiang, X., Ding, S., Suo, H., Xu, C., Gao, Z., and Hu, Y.: Fabrication of chitosan-mesoporous silica SBA-15 nanocomposites via functional ionic liquid as the bridging agent for PPL immobilization. Carbohydr. Polym. 182, 245 (2018).
25.Al-Sagheer, F. and Muslim, S.: Thermal and mechanical properties of chitosan/hybrid composites. J. Nanomater. 2010, 490679 (2010).
26.Flores, C., Lopez, M., Tabary, N., Neut, C., Chai, F., Betbeder, D., Herkt, C., Cazaux, F., Gaucher, V., Martel, B., and Blanchemain, N.: Preparation and characterization of novel chitosan and β-cyclodextrin polymer sponges for wound dressing applications. Carbohydr. Polym. 173, 535 (2017).
27.Chen, J.J., Li, H.J., Zhou, X.H., Li, E.Z., Wang, Y., Guo, Y.L., and Feng, Z.S.: Efficient synthesis of hollow silica microspheres useful for porous silica ceramics. Ceram. Int. 43, 13907 (2017).
28.Zhao, Y., Yan, N., and Feng, M.W.: Thermal degradation characteristics of phenol-formaldehyde resins derived from beetle infested pine barks. Thermochim. Acta 555, 46 (2013).
29.Sinno, H. and Prakash, S.: Complements and the wound healing cascade: An updated review. Plast. Surg. Int. 2013, 146764 (2013).
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Journal of Materials Research
  • ISSN: 0884-2914
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