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Effect of sterilization processes on the properties of a silane hybrid coating applied to Ti6Al4V alloy

Published online by Cambridge University Press:  21 November 2017

Estela K. Kerstner Baldin ,
Charlene Garcia ,
João Antonio P. Henriques ,
Mariana Roesch Ely ,
Eliena Jonko Birriel ,
Rosmary Nichele Brandalise  and
Célia de Fraga Malfatti
Estela K. Kerstner Baldin*
Affiliation:
Research Laboratory Corrosion (LAPEC), University of Rio Grande do Sul (UFRGS), Porto Alegre 9500, RS, Brazil
Charlene Garcia
Affiliation:
Laboratory of Genomics, Proteomics and DNA Repair, University of Caxias do Sul (UCS), Caxias do Sul 1130, RS, Brazil
João Antonio P. Henriques
Affiliation:
Laboratory of Genomics, Proteomics and DNA Repair, University of Caxias do Sul (UCS), Caxias do Sul 1130, RS, Brazil
Mariana Roesch Ely
Affiliation:
Laboratory of Genomics, Proteomics and DNA Repair, University of Caxias do Sul (UCS), Caxias do Sul 1130, RS, Brazil
Eliena Jonko Birriel
Affiliation:
Postgraduate Program in Process and Technology Engineering, University of Caxias do Sul (UCS), Caxias do Sul 1130, RS, Brazil
Rosmary Nichele Brandalise
Affiliation:
Postgraduate Program in Process and Technology Engineering, University of Caxias do Sul (UCS), Caxias do Sul 1130, RS, Brazil
Célia de Fraga Malfatti
Affiliation:
Research Laboratory Corrosion (LAPEC), University of Rio Grande do Sul (UFRGS), Porto Alegre 9500, RS, Brazil
*
a)Address all correspondence to this author. e-mail: estela.kerstner@ufrgs.br
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Abstract

Sterilization is one of the last stages prior to the implantation of a biomaterial. Therefore, the method should be chosen carefully as this is determinant not to compromise the properties of the material. In this context, three sterilization processes were evaluated as to their effect on the properties of a silane hybrid coating: steam autoclave, ethylene oxide, and hydrogen peroxide plasma. The coating was obtained from a sol consisting of alkoxysilane Tetraethoxysilane and organoalcoxysilane Methyltriethoxysilane (MTES), applied to the Ti6Al4V substrate, to increase its corrosion resistance and biocompatibility. After sterilization, the samples were characterized by scanning electron microscopy, atomic force microscopy, profilometry, wetabillity, and Fourier transform infrared spectroscopy. The electrochemical behavior was monitored by open circuit potential and potentiodynamic polarization curves. The cytocompatibility was evaluated by adhesion, viability, and morphological alterations in the MG-63 cells. The results showed that the protective behavior of the hybrid coating was compromised regardless of the sterilization method. However, the steam autoclave caused more morphological changes on the silane hybrid coating as well as on the Ti6Al4V substrate than the other two sterilization methods. Although the sterilized hybrid coating did not show cytotoxicity, the hybrid coating sterilized by hydrogen peroxide plasma showed a higher percentage of viable cells. The ethylene oxide presented the lowest percentage of viability and the highest cell death rate.

Keywords

biomaterialTi6Al4Vsilane hybrid coatingsterilizationelectrochemical propertiescytocompatibility
Type
Articles
Information
Journal of Materials Research , Volume 33 , Issue 2 , 29 January 2018 , pp. 161 - 177
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Copyright
Copyright © Materials Research Society 2017 

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Footnotes

Contributing Editor: Amit Bandyopadhyay

References

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