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Entrapment of DFPase in titania coatings from a biomimetically derived method

Published online by Cambridge University Press:  30 March 2011

Shaun Filocamo ,
Robert Stote ,
David Ziegler  and
Heidi Gibson
Shaun Filocamo*
Affiliation:
US Army Natick Soldier Research, Development, and Engineering Center, Natick, Massachusetts 01760
Robert Stote
Affiliation:
US Army Natick Soldier Research, Development, and Engineering Center, Natick, Massachusetts 01760
David Ziegler
Affiliation:
US Army Natick Soldier Research, Development, and Engineering Center, Natick, Massachusetts 01760
Heidi Gibson
Affiliation:
US Army Natick Soldier Research, Development, and Engineering Center, Natick, Massachusetts 01760
*
a)Address all correspondence to this author. e-mail: Shaun.Filocamo@us.army.mil
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Abstract

Silicon oxide has been widely used to encapsulate biomolecules to preserve their activity in less than ideal environments. However, there are other inorganic oxides with inherent properties that would be advantageous in creating a multifunctional material. Titanium oxide exhibits properties that have applications in areas such as electronics, energy conversion, and decontamination. Herein is reported the formation of titania coatings fabricated on polymer beads using a biomimetic approach and characterized with scanning electron microscopy and energy dispersive x-ray spectroscopy. The approach involves the use of functionalized polymer beads, which initiate oxide formation from a water-soluble titanium complex. The method was used to encapsulate the enzyme diisopropylfluorophosphatase, in situ, within the oxide matrix under buffered aqueous conditions while retaining its enzymatic activity against diisopropylfluorophosphate. In addition, the biomimetically produced titania was shown to exhibit UV-assisted degradation activity against an ethidium bromide dye, upon liberation from the coating template.

Keywords

Biomimetic (chemical reaction)CoatingOxide
Type
Articles
Information
Journal of Materials Research , Volume 26 , Issue 8 , 28 April 2011 , pp. 1042 - 1051
Copyright
Copyright © Materials Research Society 2011

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