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G-protein-coupled receptors function as logic gates for nanoparticle binding using systems and synthetic biology approach

  • Aman Chandra Kaushik (a1), Xueying Mao (a2), Cheng-Dong Li (a1), Yan Li (a3), Dong-Qing Wei (a1) and Shakti Sahi (a4)...

Abstract

G-protein-coupled receptor 142 (GPR142) belongs to rhodopsin family. GPR142 and GPR119, both Gq-coupled receptors, are expressed in pancreatic β cells of pancreas; their activation eventually leads to triggering of insulin secretion. In this paper, through a systems and synthetic biology approach, the effect of a common hit compound has been investigated in GPR142 and GPR119 pathways. This hit that has the potential to be developed as a lead for nanodrug was obtained through high-throughput virtual screening. The hit compound was further docked with nanoparticles (GOLD, SPION, and CeO2). The probable effect of this potential hit on insulin secretion in type 2 diabetes and its dynamic behavior was explored. Kinetic simulation was performed for cross-validation of its role in both the pathways. This study opens up a probable avenue in therapy of type 2 diabetes through regulation of GPR142 and GPR119 receptors. The biological circuit constructed may further have an application as a modulator to control the up- and downregulation of the biochemical pathway and can be implemented as sensors or nanochips for therapy.

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Corresponding author

a)Address all correspondence to these authors. e-mail: dqwei@sjtu.edu.cn

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G-protein-coupled receptors function as logic gates for nanoparticle binding using systems and synthetic biology approach

  • Aman Chandra Kaushik (a1), Xueying Mao (a2), Cheng-Dong Li (a1), Yan Li (a3), Dong-Qing Wei (a1) and Shakti Sahi (a4)...

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