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Hydroxyapatite-dextran methacrylate core/shell hybrid nanocarriers for combinatorial drug therapy

Published online by Cambridge University Press:  13 August 2020

S. Ram Prasad Open the ORCID record for S. Ram Prasad [Opens in a new window] ,
A. Jayakrishnan  and
T. S. Sampath Kumar
S. Ram Prasad
Affiliation:
Biomaterials Laboratory, Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai600 036, Tamil Nadu, India Medical Materials Laboratory, Department of Metallurgical and Materials Engineering, Indian Institute of Technology Madras, Chennai600 036, Tamil Nadu, India
A. Jayakrishnan*
Affiliation:
Biomaterials Laboratory, Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai600 036, Tamil Nadu, India
T. S. Sampath Kumar*
Affiliation:
Medical Materials Laboratory, Department of Metallurgical and Materials Engineering, Indian Institute of Technology Madras, Chennai600 036, Tamil Nadu, India
*
a)Address all correspondence to these authors. e-mail: jayakrishnan1953@gmail.com; jayakrishnana@rgcb.res.in
b)e-mail: tssk@iitm.ac.in
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Abstract

In this study, a hybrid dual drug-loaded hydroxyapatite-oxidized dextran methacrylate core–shell nanocarrier was formulated and explored for combinatorial delivery of doxorubicin (DOX) and methotrexate (MTX) to bone cancer. The synthesized nanocarrier was well characterized by different techniques. In vitro drug release studies in both acidic (pH 5) and alkaline (pH 7.4) conditions showed sequential release of MTX followed by DOX in a sustained manner for 10 days. Biocompatibility and cytotoxicity studies performed using drug-loaded nanoparticles (NPs) on fibroblast L929 cells and osteosarcoma MG63 cells (OMG63) showed that the NPs were highly biocompatible and showed concentration-dependent toxicity. Gene expression studies in OMG-63 cells exhibited the upregulation of caspase-3 and BAX which confirmed the apoptosis induced by dual drug-loaded NPs. The nanocarrier is expected to be a potential bone void filling material, as well as a platform for sequential delivery of DOX and MTX for the treatment of bone cancer.

Keywords

biomaterialcore/shellnanostructure

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Type
Article
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Journal of Materials Research , Volume 35 , Issue 18 , 28 September 2020 , pp. 2451 - 2465
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Copyright © Materials Research Society 2020

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Footnotes

c)

Present address: Raja Ramanna Fellow, Rajiv Gandhi Centre for Biotechnology, Jagathy, Trivandrum 695 014, Kerala, India

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