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Rapid synthesis of hydroxyapatite nanoparticles via a novel approach in the dual-frequency ultrasonic system for specific biomedical application

Published online by Cambridge University Press:  06 May 2019

Shi-ting Deng ,
Zi-ting Lin ,
Hai-xia Tang ,
Shahid Ullah  and
Yong-guang Bi Open the ORCID record for Yong-guang Bi [Opens in a new window]
Shi-ting Deng
Affiliation:
College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China
Zi-ting Lin
Affiliation:
College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China
Hai-xia Tang
Affiliation:
College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China
Shahid Ullah
Affiliation:
Department of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen, Guangdong 518060, China
Yong-guang Bi*
Affiliation:
College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China; and The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangzhou Higher Education Mega Centre, South China University of Technology, Guangzhou 510006, China
*
a)Address all correspondence to this author. e-mail: biyongguang2002@163.com
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Abstract

The hydroxyapatite nanoparticles (nHAPs) were synthesized rapidly by the self-assembled dual-frequency ultrasonic method. The ultrasonic time and power effect on the morphology and phase composition of nHAPs were investigated through field-emission scanning electron microscopy (FE-SEM), X-ray diffraction, energy dispersive spectrometer (EDS) spectrometer, and Fourier transform infrared spectroscopy, which showed that the most uniform nanoparticles were obtained when the ultrasonic time was 30 min and the ultrasonic power was 280 W. Cytotoxicity and hemolysis tests showed that an indistinctive cytotoxic effect was within the concentration of 25–400 μg/mL and the hemolytic ratio was below 2.0% at concentration of 25–200 μg/mL, respectively, revealing a good biocompatibility of nHAPs. By loading tetracycline hydrochloride onto nHAPs spheres, the drug release results showed that the drug loading and encapsulation efficiency were (26.34 ± 2.99)% and (52.68 ± 5.98)%, respectively. The drug-loaded sample shows a slow-release property, indicating that nHAPs may be promising as drug carriers.

Keywords

hydroxyapatitedual-frequency ultrasoundbiological materials

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Type
Article
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Journal of Materials Research , Volume 34 , Issue 16 , 28 August 2019 , pp. 2796 - 2806
Copyright
Copyright © Materials Research Society 2019 

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