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A simple in situ synthesis of iron oxide magnetic nanoparticles embedded in thermosensitive polymer for DNA capture

Published online by Cambridge University Press:  03 August 2020

Sadia Hossain ,
Mahbubor Rahman Open the ORCID record for Mahbubor Rahman [Opens in a new window] ,
Yeasmin Nahar ,
Abdur Rahman ,
Mostafa Kaiyum Sharafat ,
Motahar Hossain ,
Bungo Ochiai ,
Abdelhamid Elaissari  and
Hasan Ahmad
Sadia Hossain
Affiliation:
Research Laboratory of Polymer Colloids & Nanomaterials, Department of Chemistry, Faculty of Science, Rajshahi University, Rajshahi6205, Bangladesh
Mahbubor Rahman*
Affiliation:
Research Laboratory of Polymer Colloids & Nanomaterials, Department of Chemistry, Faculty of Science, Rajshahi University, Rajshahi6205, Bangladesh Advance Materials Research Laboratory, Department of Materials Science and Engineering, Clemson University, Clemson, SC29625-0971, USA
Yeasmin Nahar
Affiliation:
Research Laboratory of Polymer Colloids & Nanomaterials, Department of Chemistry, Faculty of Science, Rajshahi University, Rajshahi6205, Bangladesh
Abdur Rahman
Affiliation:
Research Laboratory of Polymer Colloids & Nanomaterials, Department of Chemistry, Faculty of Science, Rajshahi University, Rajshahi6205, Bangladesh
Mostafa Kaiyum Sharafat
Affiliation:
Department of Chemistry, Begum Rokeya University, Rangpur, Bangladesh
Motahar Hossain
Affiliation:
Research Laboratory of Polymer Colloids & Nanomaterials, Department of Chemistry, Faculty of Science, Rajshahi University, Rajshahi6205, Bangladesh
Bungo Ochiai
Affiliation:
Department of Chemistry and Chemical Engineering, Graduate School of Science and Engineering, Yamagata University, Yonezawa, Japan
Abdelhamid Elaissari
Affiliation:
Laboratory of Automatic Control, Chemical and Pharmaceutical Engineering (LAGEP)-Lyon, University of Lyon 1, Villeurbanne Cedex69622, France
Hasan Ahmad
Affiliation:
Research Laboratory of Polymer Colloids & Nanomaterials, Department of Chemistry, Faculty of Science, Rajshahi University, Rajshahi6205, Bangladesh
*
a)Address all correspondence to this author. e-mail: mrchem@ru.ac.bd
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Abstract

In this study, we report a simple one-pot synthesis of iron oxide nanoparticles (IONPs) modified with thermoresponsive polymers potentially applicable for nucleic acid capture. Ferrous (Fe2+) and ferric (Fe3+) ions were coprecipitated to a dispersion of previously prepared poly(N-isopropylacrylamide-co-2-aminoethyl methacrylate) P(NIPAAm-co-AEM) for in situ synthesis of magnetite (Fe3O4) and concurrent surface modification of Fe3O4 with the polymer to obtain magnetic nanocomposites. Fourier-transform infrared (FTIR) spectroscopy analysis reveals the surface modification of Fe3O4 with P(NIPAAm-co-AEM) and P(NIPAAm) as functional and control polymers, respectively. Fe3O4@P(NIPAAm-co-AEM) and Fe3O4@P(NIPAAm) nanocomposites’ surfaces contain 7.5 and 2.3 wt% of immobilized polymers, respectively. Vibrating sample magnetometry (VSM) result indicates a high saturation of magnetization value, 75 emu/g, for Fe3O4@P(NIPAAm-co-AEM) nanocomposites. The hydrodynamic diameter of Fe3O4@P(NIPAAm-co-AEM) in water changes depending on pH and temperature. A study for deoxyribonucleic acid (DNA) capture ability of Fe3O4@P(NIPAAm-co-AEM) nanocomposites shows a maximum 18.5 mg/g of DNA can be adsorbed on Fe3O4@P(NIPAAm-co-AEM).

Keywords

iron oxide nanoparticlesin situ synthesissurface modificationthermosensitive polymermagnetic nanocompositeDNA adsorption
Type
Article
Information
Journal of Materials Research , Volume 35 , Issue 18 , 28 September 2020 , pp. 2441 - 2450
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Copyright
Copyright © Materials Research Society 2020

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