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Upconversion rare earth nanoparticles functionalized with folic acid for bioimaging of MCF-7 breast cancer cells

Published online by Cambridge University Press:  26 December 2017

Dalia Chávez-García ,
Karla Juárez-Moreno ,
Cristian H. Campos ,
Joel B. Alderete  and
Gustavo A. Hirata
Dalia Chávez-García*
Affiliation:
Centro de Enseñanza Técnica y Superior, Campus Ensenada, Ensenada, Baja California C.P. 22860, México
Karla Juárez-Moreno
Affiliation:
Centro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México, Ensenada, Baja California C.P. 22860, México; and CONACYT Research Fellow at Centro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México, Ensenada, Baja California C.P. 22860, Mexico
Cristian H. Campos
Affiliation:
Facultad de Ciencias Químicas, Universidad de Concepción, Concepción C.P. 4070386, Chile
Joel B. Alderete
Affiliation:
Facultad de Ciencias Químicas, Universidad de Concepción, Concepción C.P. 4070386, Chile
Gustavo A. Hirata
Affiliation:
Centro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México, Ensenada, Baja California C.P. 22860, México
*
a)Address all correspondence to this author. e-mail: dalia.chavez@cetys.mx
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Abstract

Luminescent biolabels are being eagerly investigated as a means of detecting cancer cells by bioimaging. Upconversion nanoparticles are a promising option to be used as biolabels for cancer cell detection. This process uses a near infrared beam (NIR λ = 980 nm) as the excitation source to upconvert the energy into light in the visible region. The present study, used Y2O3:Yb3+, Er3+ (1%, 10% mol) and Gd2O3:Yb3+, Er3+ (1%, 10% mol) capable of emitting red photons of λ = 660 nm. The nanoparticles were previously functionalized with aminosilanes and folic acid (UCNP-NH2-FA). Folic acid binds to the folate receptor on the surface of MCF-7 breast cancer cells, and this binding promotes internalization of the UCNPs via endocytosis. The UCNPs were characterized by TEM, EDS, and Fourier transform infrared. Cytotoxicity was also analyzed using the MTT (methy-134 thiazolyltetrazolium) colorimetric assay. The UCNPs-NH2-FA was noncytotoxic to the studied cancer cells and they were clearly localizable within the cell cytoplasm via confocal microscope.

Keywords

luminescencelanthanidebiomedical
Type
Invited Paper
Information
Journal of Materials Research , Volume 33 , Issue 2 , 29 January 2018 , pp. 191 - 200
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Copyright
Copyright © Materials Research Society 2017 

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Footnotes

Contributing Editor: Lakshmi Nair

References

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