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Yogurt drink fortified with menaquinone-7 improves vitamin K status in a healthy population

  • Marjo H. J. Knapen (a1), Lavienja A. J. L. M. Braam (a1), Kirsten J. Teunissen (a1), Renate M. L. Zwijsen (a2), Elke Theuwissen (a1) and Cees Vermeer (a1)...
Abstract

Population-based studies have shown an inverse association between dietary menaquinones (MK-n, vitamin K2) intake, coronary calcification and CHD risk, suggesting a potential role of vitamin K in vascular health. To date, the effects of increased menaquinone intake on (markers of) vascular health have been investigated using predominantly food supplements. Dairy products contain many essential nutrients and can serve as a good matrix for food fortification in order to support health. We were therefore interested to study the effects of a menaquinone-fortified yogurt drink (menaquinone as menaquinone-7 (MK-7); 28 µg MK-7/yogurt drink) on vitamin K status and markers of vascular health. The yogurt drink was also fortified with n-3 PUFA, vitamin D, vitamin C, Ca and Mg to support vascular and/or general health. Healthy men (n 32) and postmenopausal women (n 28) with a mean age of 56 (sd 5) years received either basic or fortified yogurt drink twice per d for 12 weeks. MK-7 was efficiently absorbed from the fortified yogurt drink. Levels of circulating MK-7 were significantly increased from 0·28 to 1·94 ng/ml. In accordance, intake of the fortified yogurt drink improved vitamin K status, as measured by significant decreases in uncarboxylated osteocalcin and desphospho-uncarboxylated matrix Gla-protein. No effects were, however, seen on markers of inflammation, endothelial dysfunction and lipid metabolism. In summary, consumption of a yogurt drink fortified with low doses of among others MK-7 for 3 months significantly improved vitamin K status in a healthy population.

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This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
Corresponding author
* Corresponding author: Dr C. Vermeer, fax +31 433885889, email c.vermeer@vitak.com
References
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1. Geleijnse, JM, Vermeer, C, Grobbee, DE, et al. (2004) Dietary intake of menaquinone is associated with a reduced risk of coronary heart disease: the Rotterdam Study. J Nutr 134, 31003105.
2. Beulens, JW, Bots, ML, Atsma, F, et al. (2009) High dietary menaquinone intake is associated with reduced coronary calcification. Atherosclerosis 203, 489493.
3. Gast, GC, de Roos, NM, Sluijs, I, et al. (2009) A high menaquinone intake reduces the incidence of coronary heart disease. Nutr Metab Cardiovasc Dis 19, 504510.
4. Vermeer, C (1990) γ-Carboxyglutamate-containing proteins and the vitamin K-dependent carboxylase. Biochem J 266, 625636.
5. Suttie, JW (1993) Synthesis of vitamin K-dependent proteins. FASEB J 7, 445452.
6. Furie, B, Bouchard, BA & Furie, BC (1999) Vitamin K-dependent biosynthesis of γ-carboxyglutamic acid. Blood 93, 17981808.
7. Theuwissen, E, Cranenburg, EC, Knapen, MH, et al. (2012) Low-dose menaquinone-7 supplementation improved extra-hepatic vitamin K status, but had no effect on thrombin generation in healthy subjects. Br J Nutr 108, 16521657.
8. Shea, MK, O'Donnell, CJ, Vermeer, C, et al. (2011) Circulating uncarboxylated matrix Gla-protein is associated with vitamin K nutritional status, but not coronary artery calcium, in older adults. J Nutr 141, 15291534.
9. Brugè, F, Bacchetti, T, Principi, F, et al. (2011) Olive oil supplemented with menaquinone-7 significantly affects osteocalcin carboxylation. Br J Nutr 106, 10561062.
10. Dalmeijer, GW, van der Schouw, YT, Magdeleyns, E, et al. (2012) The effect of menaquinone-7 supplementation on circulating species of matrix Gla-protein. Atherosclerosis 225, 397402.
11. Szulc, P, Chapuy, MC, Meunier, PJ, et al. (1996) Serum undercarboxylated osteocalcin is a marker of the risk of hip fracture in elderly women. J Clin Invest 9, 17691774.
12. Szulc, P, Arlot, M, Chapuy, MC, et al. (1994) Serum undercarboxylated osteocalcin correlates with hip bone mineral density in elderly women. J Bone Miner Res 9, 15911595.
13. Booth, SL, Broe, KE, Peterson, JW, et al. (2004) Associations between vitamin K biochemical measures and bone mineral density in men and women. J Clin Endocrinol Metab 89, 49044909.
14. Vergnaud, P, Garnero, P, Meunier, PJ, et al. (1997) Undercarboxylated osteocalcin measured with a specific immunoassay predicts hip fracture in elderly women: the EPIDOS study. J Clin Endocrinol Metab 82, 719724.
15. Knapen, MHJ, Nieuwenhuijzen Kruseman, AC, Wouters, RSME, et al. (1998) Correlation of serum osteocalcin fractions with bone mineral density in women during the first 10 years after menopause. Calc Tissue Int 63, 375379.
16. Luukinen, H, Käkönen, SM, Pettersson, K, et al. (2000) Strong prediction of fractures among older adults by the ratio of carboxylated to total serum osteocalcin. J Bone Miner Res 15, 24732478.
17. Cranenburg, EC, Vermeer, C, Koos, R, et al. (2008) The circulating inactive form of matrix Gla protein (ucMGP) as a biomarker for cardiovascular calcification. J Vasc Res 45, 427436.
18. Cranenburg, EC, Koos, R, Schurgers, LJ, et al. (2010) Characterisation and potential diagnostic value of circulating matrix Gla protein (MGP) species. Thromb Haemost 104, 811822.
19. Rennenberg, RJ, de Leeuw, PW, Kessels, AG, et al. (2010) Calcium scores and matrix Gla-protein levels: association with vitamin K status. Eur J Clin Invest 40, 344349.
20. Ueland, T, Dahl, CP, Gullestad, L, et al. (2011) Circulating levels of non-phosphorylated undercarboxylated matrix Gla-protein are associated with disease severity in patients with chronic heart failure. Clin Sci 121, 119127.
21. Sokoll, LJ, Booth, SL, O'Brien, ME, et al. (1997) Changes in serum osteocalcin, plasma phylloquinone, and urinary γ-carboxyglutamic acid in response to altered intakes of dietary phylloquinone in human subjects. Am J Clin Nutr 65, 779784.
22. Booth, SL, O'Brien-Morse, ME, Dallal, GE, et al. (1999) Response of vitamin K status to different intakes and sources of phylloquinone-rich foods: comparison of younger and older adults. Am J Clin Nutr 70, 368377.
23. Westenfeld, R, Krueger, T, Schlieper, G, et al. (2012) Effect of vitamin K2 supplementation on functional vitamin K deficiency in hemodialysis patients: a randomized trial. Am J Kidney Dis 59, 186195.
24. Booth, SL, Sadowski, JA, Weihrauch, JL, et al. (1993) Vitamin K1 (phylloquinone) content of foods: a provisional table. J Food Comp Anal 6, 109120.
25. Shearer, MJ & Bolton-Smith, C (2000) The U.K. food data-base for vitamin K and why we need it. Food Chem 68, 213218.
26. Schurgers, LJ & Vermeer, C (2000) Determination of phylloquinone and menaquinones in food. Effect of food matrix on circulating vitamin K concentrations. Haemostasis 30, 298307.
27. Gaucheron, F (2011) Milk and dairy products: a unique micronutrient combination. J Am Coll Nutr 30, 400S409S.
28. Kromhout, D, Bosschieter, EB & de Coulander, CL (1987) The inverse relation between fish consumption and 2-year mortality from coronary heart disease. Lancet i, 177183.
29. Burr, ML, Fehily, AM, Gilbert, JF, et al. (1989) Effects of changes in fat, fish and fibre intakes on death and myocardial reinfarction. Lancet ii, 757761.
30. Kris-Etherton, PM, Harris, WS & Appel, LJ (2003) Fish consumption, fish oil, omega-3 fatty acids, and cardiovascular disease. Arterioscler Thromb Vasc Biol 23, 151152.
31. Friedewald, WT, Levy, RI & Fredrickson, DS (1972) Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18, 499502.
32. Kanellakis, S, Moschonis, G, Tenta, R, et al. (2012) Changes in parameters of bone metabolism in postmenopausal women following a 12-month intervention period using dairy products enriched with calcium, vitamin D, and phylloquinone (vitamin K1) or menaquinone-7 (vitamin K2): the Postmenopausal Health Study II. Calc Tissue Int 90, 251262.
33. Braam, LA, Knapen, MH, Geusens, P, et al. (2003) Vitamin K1 supplementation retards bone loss in postmenopausal women between 50 and 60 years of age. Calc Tissue Int 73, 2126.
34. Bolton-Smith, C, McMurdo, ME, Paterson, CR, et al. (2007) Two-year randomized controlled trial of vitamin K1 (phylloquinone) and vitamin D3 plus calcium on the bone health of older women. J Bone Miner Res 22, 509519.
35. Booth, SL, Dallal, G, Shea, MK, et al. (2008) Effect of vitamin K supplementation on bone loss in elderly men and women. J Clin Endocrinol Metab 93, 12171223.
36. Emaus, N, Gjesdal, CG, Almas, B, et al. (2009) Vitamin K2 supplementation does not influence bone loss in early menopausal women: a randomised double-blind placebo-controlled trial. Osteoporos Int 21, 17311740.
37. Knapen, MH, Schurgers, LJ & Vermeer, C (2007) Vitamin K2 supplementation improves hip bone geometry and bone strength indices in postmenopausal women. Osteoporos Int 18, 963972.
38. Bugel, S, Sorensen, AD, Hels, O, et al. (2007) Effect of phylloquinone supplementation on biochemical markers of vitamin K status and bone turnover in postmenopausal women. Br J Nutr 97, 373380.
39. Cheung, AM, Tile, L, Lee, Y, et al. (2008) Vitamin K supplementation in postmenopausal women with osteopenia (ECKO trial): a randomized controlled trial. PLoS Med 5, e196.
40. Binkley, N, Harke, J, Krueger, D, et al. (2009) Vitamin K treatment reduces undercarboxylated osteocalcin but does not alter bone turnover, density, or geometry in healthy postmenopausal North American women. J Bone Miner Res 24, 983991.
41. van Summeren, MJ, Braam, LA, Lilien, MR, et al. (2009) The effect of menaquinone-7 (vitamin K2) supplementation on osteocalcin carboxylation in healthy prepubertal children. Br J Nutr 102, 11711178.
42. Schurgers, LJ, Teunissen, KJ, Hamulyak, K, et al. (2007) Vitamin K-containing dietary supplements: comparison of synthetic vitamin K1 and natto-derived menaquinone-7. Blood 109, 32793283.
43. Shearer, MJ, Fu, X & Booth, SL (2012) Vitamin K nutrition, metabolism, and requirements: current concepts and future research. Adv Nutr 3, 182195.
44. Shea, MK, Dallal, GE, Dawson-Hughes, B, et al. (2008) Vitamin K, circulating cytokines, and bone mineral density in older men and women. Am J Clin Nutr 88, 356363.
45. Shea, MK, Booth, SL, Massaro, JM, et al. (2008) Vitamin K and vitamin D status: associations with inflammatory markers in the Framingham Offspring Study. Am J Epidemiol 167, 313320.
46. Juanola-Falgarona, M, Salas-Salvadó, J, Estruch, R, et al. (2013) Association between dietary phylloquinone intake and peripheral metabolic risk markers related to insulin resistance and diabetes in elderly subjects at high cardiovascular risk. Cardiovasc Diabetol 12, 7.
47. Ebina, K, Shi, K, Hirao, M, et al. (2013) Vitamin K2 administration is associated with decreased disease activity in patients with rheumatoid arthritis. Mod Rheumatol 23, 10011007.
48. Rangel-Huerta, OD, Aguilera, CM, Mesa, MD, et al. (2012) Omega-3 long-chain polyunsaturated fatty acids supplementation on inflammatory biomarkers: a systematic review of randomized clinical trials. Br J Nutr 107, S159S170.
49. Yusof, HM, Miles, E & Calder, P (2008) Influence of very long-chain n-3 fatty acids on plasma markers of inflammation in middle-aged men. Prostaglandins Leukot Essent Fatty Acids 78, 219228.
50. Thies, F, Miles, E, Nebe-von-Caron, G, et al. (2001) Influence of dietary supplementation with long-chain n-3 or n-6 polyunsaturated fatty acids on blood inflammatory cell populations and functions and on plasma soluble adhesion molecules in healthy adults. Lipids 36, 11861193.
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