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Characterization of the in vitro production of N-acyl homoserine lactones by cultivable bacteria inhabiting the sponge Suberites domuncula

Published online by Cambridge University Press:  24 February 2016

Jasnizat Bin Saidin ,
Mohd Effendy Abd Wahid  and
Gaël Le Pennec
Jasnizat Bin Saidin
Affiliation:
Universiti Malaysia Terengganu, IMB, Kuala Terengganu, Terengganu, Malaysia Université de Bretagne-Sud, EA 3884, LBCM, IUEM, F-56100 Lorient, France
Mohd Effendy Abd Wahid
Affiliation:
Universiti Malaysia Terengganu, IMB, Kuala Terengganu, Terengganu, Malaysia
Gaël Le Pennec*
Affiliation:
Université de Bretagne-Sud, EA 3884, LBCM, IUEM, F-56100 Lorient, France
*
Correspondence should be addressed to:G. Le Pennec, Laboratoire de Biotechnologie et Chimie Marines, Université de Bretagne-Sud, BP 92116, 56321 Lorient Cedex, France email: gael.le-pennec@univ-ubs.fr
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Abstract

Sponges together with associated bacteria form complex holobionts governed by various relationships established between the partners. Molecules of communication may participate in the homeostasis of these biological associations. Bacteria produce N-acyl homoserine lactones to communicate. In the present study, we characterized 102 cultivable bacteria isolated from the sponge Suberites domuncula at the genus level and described their production of AHLs during 96 h of culture in a Marine Broth medium. The presence of AHLs was monitored using the Escherichia coli pSB406 reporter strain. Sixty-seven bacteria were AHLs positive. Among them, 46 were related to the Gammaproteobacteria, eight to the Alphaproteobacteria, seven to the Firmicutes and six to the Flavobacteria. The kinetic production of AHLs was determined and compared with a reference bacterium, Pseudomonas aeruginosa PAO1 (pDA224). We established three profiles of AHLs production with no definitive pattern related to an order, a family or a genus of bacteria.

Keywords

N-Acyl homoserine lactonePoriferaSuberites domunculacultivable bacteriasymbiosis
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 97 , Issue 1 , February 2017 , pp. 119 - 127
Copyright
Copyright © Marine Biological Association of the United Kingdom 2016 

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