Skip to main content
    • Aa
    • Aa

COI sequencing as tool for the taxonomic attribution of Patella spp. (Gastropoda): the case of morphologically undistinguishable juveniles settled on a Patella ferruginea adult

  • Marco Casu (a1), Daria Sanna (a1), Benedetto Cristo (a1), Tiziana Lai (a1), Gian Luca Dedola (a1) and Marco Curini-Galletti (a1)...

Patella ferruginea is an endangered marine gastropod, distributed on the western Mediterranean coasts, whose range has progressively contracted, due to intense human exploitation. A genetic analysis was performed on two unidentified young individuals belonging to the genus Patella found attached to the shell of an adult of P. ferruginea, with a twofold aim: (i) to achieve their correct taxonomic attribution by means of the DNA barcoding; and (ii) to shed some light on the hypothesized larval philopatry and/or juveniles phoresis in P. ferruginea. The survey was carried out comparing the sequences of the cytochrome c oxidase subunit I (COI) Folmer region obtained for the two juveniles with those obtained for adults of P. ferruginea, P. caerulea, P. rustica, and P. ulyssiponensis, from different sites of the western Mediterranean, by means of maximum likelihood cluster analysis and a Bayesian-based assignment test. Results obtained evidenced that: (i) COI may be used with confidence as DNA barcoding in the genus Patella; and (ii) the two juveniles studied are not conspecific: one belonged to P. ferruginea, the other to P. rustica. The latter finding raises doubts about the juvenile phoresis and about the occurrence of larval philopatry in P. ferruginea, suggesting that an extensive use of a molecular approach for a better evaluation of the recruitment features of this endangered species should be adopted.

Corresponding author
Correspondence should be addressed to: M. Casu, Dipartimento di Zoologia e Genetica Evoluzionistica, University of Sassari, Via Muroni 25, I-07100 Sassari, Italy email:
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

D. Bensasson , D-X. Zhang , D.L. Hartl and G.M. Hewitt (2001) Mitochondrial pseudogenes: evolution's misplaced witnesses. Trends in Ecology and Evolution 16, 314321.

M. Casu , D. Casu , T. Lai , P. Cossu and M. Curini-Galletti (2006) Inter Simple Sequence Repeat markers revealed strong genetic differentiation among populations of the endangered mollusc Patella ferruginea (Gastropoda: Patellidae) from two Sardinian Marine Protected Areas. Marine Biology 149, 11631174.

M. Casu , T. Lai , D. Sanna , P. Cossu , M. Curini-Galletti (2009) An integrative approach to the taxonomy of the pigmented European Pseudomonocelis Meixner, 1943 (Platyhelminthes: Proseriata). Biological Journal of the Linnean Society, 98, 907922.

J. Corander , P. Waldmann , P. Marttinen and M.J. Sillanpää (2004) BAPS 2: enhanced possibilities for the analysis of genetic population structure. Bioinformatics 20, 23632369.

S. Duran , M. Pascual and X. Turon (2004) Low levels of genetic variation in mtDNA sequences over the western Mediterranean and Atlantic range of the sponge Crambe crambe (Poecilosclerida). Marine Biology 144, 3135.

M.C. Ebach and C. Holdrege (2005) DNA barcoding is no substitute for taxonomy. Nature 434, 434697.

D. Erpenbeck , J.N.A. Hooper and G. Worheide (2006) CO1 phylogenies in diploblasts and the ‘Barcoding of life’—are we sequencing a suboptimal partition? Molecular Ecology Notes 6, 550553.

F. Espinosa and T. Ozawa (2006) Population genetics of the endangered limpet Patella ferruginea (Gastropoda: Patellidae): taxonomic, conservation and evolutionary considerations. Journal of Zoological Systematics and Evolutionary Research 44, 816.

F. Espinosa , J.M. Guerra García , D. Fa and J.C. García Gomez (2006) Aspects of reproduction and their implications for the conservation of the endangered limpet, Patella ferruginea. Invertebrate Reproduction and Development 49, 8592.

F. Espinosa , A.R. González , M.J. Maestre , D. Fa , J.M. Guerra-García and J.C. García Gomez (2008b) Responses of the endangered limpet Patella ferruginea to reintroduction under different environmental conditions: survival, growth rates and life-history. Italian Journal of Zoology 75, 371384.

E. Fisher-Piette (1938) The concept of species and geographical isolation in the case of north Atlantic Patella. Proceedings of the Linnean Society of London 150, 268275.

P.D.N. Hebert , S. Ratnasingham and J.R. deWaard (2003) Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proceedings of the Royal Society of London. Series B 270, 9699.

M. Klautau , C.A.M. Russo , C. Lazoski , N. Boury-Esnault , J.P. Thorpe and A.M. Solé-Cava (1999) Does cosmopolitanism result from over conservative systematics? A case study using the marine sponge Chondrilla nucula. Evolution 53, 14141422.

E. Maggi , I. Bertocci , S. Vaselli and L. Benedetti-Cecchi (2009) Effects of changes in number, identity and abundance of habitat-forming species on assemblages of rocky shores. Marine Ecology Progress Series 381, 3949.

M. Markmann and D. Tautz (2005) Reverse taxonomy: an approach towards determining the diversity of meiobenthic organisms based on ribosomal RNA signature sequences. Philosophical Transactions of the Royal Society B: Biological Sciences 360, 19171924.

A. Mauro , M. Arculeo and N. Parrinello (2003) Morphological and molecular tools in identifying the Mediterranean limpets Patella caerulea, Patella aspera and Patella rustica. Journal of Experimental Marine Biology and Ecology 295, 131143.

C.P. Meyer and G. Paulay (2005) DNA barcoding: error rates based on comprehensive sampling. PloS Biology 3, 22292238.

M.T. Monaghan , M. Balke , T.R. Gregory and A.P. Vogler (2005) DNA-based species delineation in tropical beetles using mitochondrial and nuclear markers. Philosophical Transactions of the Royal Society B: Biological Sciences 360, 19251933.

C. Moritz and C. Cicero (2004) DNA barcoding: promise and pitfalls. PLoS Biology 2, 354.

T. Nakano and T. Ozawa (2007) Worldwide phylogeography of limpets of the order patellogastropoda: molecular, morphological and palaeontological evidence. Journal of Molluscan Studies 73, 7999.

J.M. Padial , S. Castroviejo-Fisher , J. Köhler , C. Vilà , J.C. Chaparro and I. De la Riva (2009) Deciphering the products of evolution at the species level: the need for an integrative taxonomy. Zoologica Scripta 38, 431447.

D. Posada and K.A. Crandall (1998) ModelTest: testing the model of DNA substitution, version 3.7. Bioinformatics 14, 817818.

F. Rodríguez , J.F. Oliver , A. Marín and J.R. Medina (1990) The general stochastic model of nucleotide substitutions. Journal of Theoretical Biology 142, 485501.

J. Rozas and R. Rozas (1999) DnaSP version 3: an integrated program for molecular population genetics and molecular evolution analysis. Bioinformatics 15, 174175.

D. Sanna , T. Lai , P. Francalacci , M. Curini-Galletti and M. Casu (2009) Development of primers for the amplification of cytochrome c oxidase subunit I (COI) partial region (mtDNA) in Monocelis lineata (Platyhelminthes: Proseriata) species complex. Genetics and Molecular Biology 32, 864867.

A. Sá-Pinto , M. Branco , D.J. Harris and P. Alexandrino (2005) Phylogeny and phylogeography of the genus Patella based on mitochondrial DNA sequence data. Journal of Experimental Marine Biology and Ecology 325, 95110.

H.C. Schröder , S.M. Efremova , V.B. Itskovich , S. Belikov , Y. Masuda , A. Krasko , I.M. Müller and W.E.G. Müller (2003) Molecular phylogeny of the freshwater sponges in Lake Baikal. Journal of Zoological Systematics and Evolutionary Research 41, 8086.

T.L. Shearer , J.H. van Oppen , S.L. Romano and G. Worheide (2002) Slow mitochondrial DNA sequence evolution in the Anthozoa (Cnidaria). Molecular Ecology 11, 24752478.

M.D. Sorenson and T.W. Quinn (1998) Numts: a challenge for avian systematics and population biology. The Auk 115, 214221.

K. Tamura , J. Dudley , M. Nei and S. Kumar (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24, 15961599.

D. Tautz , P. Arctander , A. Minelli , R.H. Thomas and A.P. Vogler (2003) A plea for DNA taxonomy. Trends in Ecology and Evolution 18, 7074.

J.D. Thompson , D.G. Higgins and T.J. Gibson (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22, 46734680.

A.P. Vogler and M.T. Monaghan (2007) Recent advances in DNA taxonomy. Journal of Zoological Systematics and Evolutionary Research 45, 110.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Journal of the Marine Biological Association of the United Kingdom
  • ISSN: 0025-3154
  • EISSN: 1469-7769
  • URL: /core/journals/journal-of-the-marine-biological-association-of-the-united-kingdom
Please enter your name
Please enter a valid email address
Who would you like to send this to? *



Full text views

Total number of HTML views: 1
Total number of PDF views: 4 *
Loading metrics...

Abstract views

Total abstract views: 62 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 30th May 2017. This data will be updated every 24 hours.