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Comparative analysis of biofilm community on different coloured substrata in relation to mussel settlement

Published online by Cambridge University Press:  12 February 2016

Yi-Feng Li ,
Xing-Pan Guo ,
Yu-Ru Chen ,
De-Wen Ding  and
Jin-Long Yang
Yi-Feng Li
Affiliation:
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China
Xing-Pan Guo
Affiliation:
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China
Yu-Ru Chen
Affiliation:
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China
De-Wen Ding
Affiliation:
Marine Ecology Research Center, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China
Jin-Long Yang*
Affiliation:
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China Marine Ecology Research Center, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo 315211, China
*
Correspondence should be addressed to:J.L. Yang, Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China email: jlyang@shou.edu.cn
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Abstract

Mussels are typical macrofouling organisms in the world. In this study, the interaction between the settlement of Mytilus coruscus plantigrades and bacterial community on coloured substrata was determined. Bacterial communities in biofilms developed on seven coloured substrata were analysed by Illumina Miseq sequencing. The mussel settlement response to coloured substrata with no biofilms was also examined. Flavobacteria, Alphaproteobacteria and Gammaproteobacteria were the first, second and third most dominant groups in seven biofilm samples. The results suggest that the inducing activities of these biofilms on plantigrade settlement varied with coloured substrata and the lowest percentage of settlement was observed on biofilms on the green substratum. High-throughput sequencing showed that bacterial community in biofilms also changed with the substratum colour. No significant difference in the inducing activity on plantigrade settlement was observed between the coloured substrata with no biofilms. Thus, difference in plantigrade settlement response may be correlated to the changes in bacterial community on coloured substrata. This finding extends current knowledge of interaction among mussel settlement and bacterial community variability.

Keywords

Mytilus coruscusmussel settlementbacterial communitycoloured substrataMiseq sequencing
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 97 , Issue 1 , February 2017 , pp. 81 - 89
Copyright
Copyright © Marine Biological Association of the United Kingdom 2016 

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