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Comparative study of the composition and genetic diversity of the picoeukaryote community in a Chinese aquaculture area and an open sea area

Published online by Cambridge University Press:  01 March 2016

Xue Song ,
Zhimeng Xu ,
Qian Liu ,
Yan Li ,
Yu Ma ,
Jian Wang ,
Mengran Sun ,
Hongbing Shao ,
Hui Sun  and
Gill Malin
...Show all authors
Xue Song
Affiliation:
College of Marine Life Science, Ocean University of China, Qingdao 266003, P.R. China
Zhimeng Xu
Affiliation:
College of Marine Life Science, Ocean University of China, Qingdao 266003, P.R. China
Qian Liu
Affiliation:
College of Marine Life Science, Ocean University of China, Qingdao 266003, P.R. China
Yan Li
Affiliation:
College of Marine Life Science, Ocean University of China, Qingdao 266003, P.R. China
Yu Ma
Affiliation:
College of Marine Life Science, Ocean University of China, Qingdao 266003, P.R. China
Jian Wang
Affiliation:
College of Marine Life Science, Ocean University of China, Qingdao 266003, P.R. China
Mengran Sun
Affiliation:
College of Marine Life Science, Ocean University of China, Qingdao 266003, P.R. China
Hongbing Shao
Affiliation:
College of Marine Life Science, Ocean University of China, Qingdao 266003, P.R. China
Hui Sun
Affiliation:
College of Marine Life Science, Ocean University of China, Qingdao 266003, P.R. China
Gill Malin
Affiliation:
School of Environmental Sciences, University of East Anglia, Norwich NR4 7TJ, UK
Yong Jiang*
Affiliation:
College of Marine Life Science, Ocean University of China, Qingdao 266003, P.R. China
Min Wang*
Affiliation:
College of Marine Life Science, Ocean University of China, Qingdao 266003, P.R. China
*
Correspondence should be addressed to:Y. Jiang and M. Wang, College of Marine Life Science, Ocean University of China, Qingdao 266003, P.R. China email: mingwang@ouc.edu.cnyongjiang@ouc.edu.cn
Correspondence should be addressed to:Y. Jiang and M. Wang, College of Marine Life Science, Ocean University of China, Qingdao 266003, P.R. China email: mingwang@ouc.edu.cnyongjiang@ouc.edu.cn
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Abstract

Picoeukaryotes (<2–3 μm) perform key roles for the functioning of marine ecosystems, but little is known regarding the composition and diversity of picoeukaryotes in aquaculture areas. In this study, the Illumina MiSeq platform was used for sequencing the V4 variable region within the 18S rDNA gene to analyse genetic diversity and relative abundance of picoeukaryotic communities in the Qinhuangdao scallop cultivation area of the Bohai Sea. The community was dominated by three super groups, the alveolates (54%), stramenopiles (41%) and chlorophytes (3%), and three groups, dinoflagellates (54%), pelagomonadales (40%) and prasinophytes (3%). Furthermore, a contrasting station with open water away from the eutrophic aquaculture area was chosen. The communities collected from the two stations exhibited significant differences, with higher diversity in the aquaculture area. These results provide the first snapshot of the picoeukaryotic diversity in surface waters of the Qinhuangdao scallop cultivation area, and basic data for future studies on picoeukaryote community in an aquaculture region.

Keywords

environmental heterogeneitygenetic diversitypicoeukaryotesMiSeq18S rDNA
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 97 , Issue 1 , February 2017 , pp. 151 - 159
Copyright
Copyright © Marine Biological Association of the United Kingdom 2016 

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