Skip to main content
    • Aa
    • Aa

Diurnal changes of photoadaptive pigments in microphytobenthos

  • Leonie Jordan (a1), Andrew McMinn (a1) and Peter Thompson (a2)

Microphytobenthos need photoadaptive strategies to survive the highly dynamic light environment in which they reside. Xanthophyll pigments can provide photoprotection by cycling electrons between epoxide and de-epoxide forms, dissipating excess light energy as heat. This study examined the xanthophyll cycle in microphytobenthos on a tidally exposed substrate at Browns River, Tasmania. Fv/Fm decreased from 0.52±0.01 to 0.47±0.01 at noon in surface samples and a decrease in the diadinoxanthin:chlorophyll-a ratio from 0.022±0.003 to 0.015±0.005 also suggests that the microphytobenthos was under physiological stress at noon. The results indicate that the cells exposed to light at the surface migrated deeper into the sediments and replenished the epoxide form of their xanthophylls. The results suggest that micrphytobenthos utilizes both behavioural and physiological strategies to survive in the dynamic intertidal environment.

Corresponding author
Correspondence should be addressed to: Andrew McMinn, Institute of Antarctic and Southern Ocean studies, University of Tasmania, Box 252-77, Hobart 7001, Tasmania, Australia email:
Hide All
T. Anning , G. Harris and R. Geider (2001) Thermal acclimation in the marine diatom Chaetoceros calcitrans (Bascillariophyta). European Journal of Phycology 36, 233241.

H. Claustre , P. Kerherve , J.C. Marty and L. Prieur (1994) Phytoplankton photoadaptation related to some frontal physical processes. Journal of Marine Systems 5, 251265.

M. Consalvey , D.M. Paterson and G.J.C. Underwood (2004) The ups and downs of life in a benthic biofilm: migration of benthic diatoms. Diatom Research 19, 181202.

S. Cruz and J. Serôdio (2008) Relationship of rapid light curves of variable fluorescence to photoacclimation and non-photochemical quenching in a benthic diatom. Aquatic Botany 88, 256264.

A.W. Decho (2000) Microbial biofilms in intertidal systems: an overview. Continental Shelf Research 20, 12571273.

M. Eskling , P.O. Arvidsson and H.E. Akerlund (1997) The xanthophyll cycle, its regulation and components. Physiologia Plantarum 100, 806816.

T. Fujiki , T. Toda , T. Kikuchi and S. Taguchi (2003) Photoprotective response of xanthophyll pigments during phytoplankton blooms in Sagami Bay, Japan. Journal of Plankton Research 25, 317322.

B. Genty , J.M. Briantais and N.R. Baker (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochimica et Biophysica Acta 990, 8792.

A.G. Ivanov , P. Sane , V. Hurry , M. Krol , D. Sveshnikor , N.P.A. Huner and G. Oquist (2003) Low temperature modulation of the redox properties of the acceptor side of photosystem II: Photoprotection through reaction centre quenching of excess energy. Physiologia Plantarium 119, 376383.

A.D. Jassby and T. Platt (1976) Mathematical formulation of the relationship between photosynthesis and light for phytoplankton. Limnology and Oceanography 21, 540547.

W.J. Jeong , Y.I. Park , K.H. Suh , J.A. Raven , O.J. Yoo and Y.R. Liu (2002) A large population of small chloroplasts in tobacco leaf cells allows more effective chloroplast movement than a few enlarged chloroplasts. Plant Physiology 129, 112121.

B. Jesus , R.G. Perkins , M. Consalvey , V. Brotas and D.M. Paterson (2006) Effects of vertical migrations by benthic microalgae on fluorescence measurements of photophysiology. Marine Ecology Progress Series 315, 5566.

L. Jordan , A. McMinn and S. Wotherspoon (2008) Diurnal and monthly vertical profiles of benthic microalgae within intertidal sediments from two temperate localities. Marine and Freshwater Research 59, 931939.

M.B. Kingston (1999) Effect of light on vertical migration and photosynthesis of Euglena proxima (Euglenophyta). Journal of Phycology 35, 245253.

C.H. Koh , J.S. Khim , H. Araki , H. Yamanishi and K. Koga (2007) Within-day and seasonal patterns of microphytobenthos biomass determined by co-measurement of sediment and water column chlorophylls in the intertidal mudflat of Nanaura, Saga, Ariake Sea, Japan. Estuarine, Coastal and Shelf Science 72, 4252.

Z. Kolber and P.G. Falkowski (1993) Use of active fluorescence to estimate phytoplankton photosynthesis in situ. Limnology and Oceanography 38, 16461665.

G.H. Krause and E. Weiss (1991) Chlorophyll fluorescence and photosynthesis: the basics. Annual Review of Plant Physiology and Plant Molecular Biology 42, 313349.

J. Lavaud , B. Rousseau and A.L. Etienne (2004) General features of photoprotection by energy dissipation in planktonic diatoms (Bacillariophyceae). Journal of Phycology 40, 130137.

M. Lohr and C. Wilhelm (2001) Xanthophyll synthesis in diatoms: quantification of putative intermediates and comparison of pigment conversion kinetics with rate constants derived from a model. Planta 212, 382391.

N.W. Lomas and P.M. Gilbert (1999) Interactions between NH4+ and NO3 uptake and assimilations: comparison of diatoms and dinoflagellates at several growth temperatures. Marine Biology 133, 541551.

M.D. Mackey , D.J. Mackey , H.W. Higgins and S.W. Wright (1996) CHEMTAX—a program for estimating class abundances from chemical markers: application to HPLC measurements of phytoplankton. Marine Ecology Progress Series 144, 265283.

T.A. Moisan , M. Olaizola and B.G. Mitchell (1998) Xanthophyll cycling in Phaeocystis antarctica: changes in cellular fluorescence. Marine Ecology Progress Series 169, 113121.

P. Muller , X.P. Li and K.K. Niyogi (2001) Non-photochemical quenching: a response to excess light energy. Plant Physiology 125, 15581566.

R.G. Perkins , K. Oxborough , A.R.M. Hanlon , G.J.C. Underwood and N.R. Baker (2002) Can chlorophyll fluorescence be used to estimate the rate of photosynthetic electron transport within microphytobenthic biofilms? Marine Ecology Progress Series 228, 4756.

P. Ralph and R. Gademann (2005) Rapid light curves: a powerful tool to assess photosynthetic activity. Aquatic Botany 82, 222237.

O. Schofield , T.J. Evens and D.F. Millie (1998) Photosystem II quantum yields and xanthophyll cycle pigments of the macroalga Sargassum natans (Phaeophyceae): responses under natural sunlight. Journal of Phycology 34, 104112.

J. Serôdio , H. Coelho , S. Vieira and S. Cruz (2006) Microphytobenthos vertical migratory photoresponse as characterized by light-response curves of surface biomass. Estuarine, Coastal and Shelf Science 68, 547556.

J. Serôdio , S. Cruz , S. Vieira and V. Brotas (2005) Non-photochemical quenching of chlorophyll fluorescence and operation of the xanthophyll cycle in estuarine microphytobenthos. Journal of Experimental Marine Biology and Ecology 326, 157169.

A. Wingler , P.J. Lea , W.P. Quick and R.C. Leegood (2000) Photorespiration-metabolic pathways and their role in stress protection. Philosophical Transactions of the Royal Society of London, Series B 355, 15171529.

S.W. Wright , S.W. Jeffrey , R.F.C. Mantoura , C.A. Llewellyn , T. Bjpomland , D. Repeta and N. Welschmeyer (1991) Improved HPLC method for the analysis of chlorophylls and carotenoids from marine phytoplankton. Marine Ecology Progress Series 77, 183196.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Journal of the Marine Biological Association of the United Kingdom
  • ISSN: 0025-3154
  • EISSN: 1469-7769
  • URL: /core/journals/journal-of-the-marine-biological-association-of-the-united-kingdom
Please enter your name
Please enter a valid email address
Who would you like to send this to? *



Full text views

Total number of HTML views: 1
Total number of PDF views: 5 *
Loading metrics...

Abstract views

Total abstract views: 99 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 18th October 2017. This data will be updated every 24 hours.