Skip to main content

Crustacea in the Diet of Eledone Cirrhosa (Mollusca: Cephalopoda) determined by Serological Methods

  • P. R. Boyle (a1), M. S. Grisley (a1) and G. Robertson (a1)

Serological methods for prey identification have been applied to the gut contents of a field sample of 100 Eledone cirrhosa from the Moray Firth and 7 from the Sound of Jura. Protein extracts from the crop were electrophoresed (Laurell rockets) into antisera raised against potential crustacean prey species, Cancer pagurus, Carcinus maenas, Crangon crangon, Liocarcinus spp. and Nephrops norvegicus. The strengths of the resulting reactions were evaluated on the basis of peak height, staining density and the number of homocentric peaks of immune precipitates. Sixty-two of the 100 Moray Firth animals gave a positive reaction to one or more of the antisera and the most critical assessment of the results ranked the incidence of the prey as Liocarcinus (15) > Nephrops (10) > Cancer (8) > Crangon (2) > Carcinus (0), a total of 35 reactions from 28 animals. Absence of a reaction in the 38 animals from which appreciable sample volumes were also obtained indicates that alternative prey had been consumed. Of the 7 Sound of Jura animals, 4 gave a positive reaction to Nephrops alone. A total of only 24 animals gave any indication of diet by visual recognition of remains. The value and difficulties of the methodology are discussed.

Hide All
Ambrose, R. F., 1983. Midden formation by octopuses: the role of biotic and abiotic factors. Marine Behaviour and Physiology, 10, 137144.
Ambrose, R. F., 1984. Food preferences, prey availability, and the diet of Octopus bimaculatus Verrill. Journal of Experimental Marine Biology and Ecology, 77, 2944.
Ambrose, R. F. & Nelson, B. V., 1983. Predation by Octopus vulgaris in the Mediterranean. Marine Ecology, 4, 251261.
Boyle, P. R., 1986. A descriptive ecology of Eledone cirrhosa (Mollusca: Cephalopoda) in Scottish waters. Journal of the Marine Biological Association of the United Kingdom, 66, 855865.
Clarke, H. G. M. & Freeman, T., 1966. A quantitative immunoelectrophoresis method (Laurell electrophoresis). Protides of the Biological Fluids, 14, 503509.
Feller, R. J. & Gallagher, E. D., 1982. Antigenic similarities among estuarine soft-bottom benthic taxa. Oecologia, 52, 305310.
Feller, R. J., Taghon, G. L., Gallagher, E. D., Kenny, G. E. & Jumars, P. A., 1979. Immunological methods of food web analysis in a soft-bottom benthic community. Marine Biology, 54, 6174.
Grisley, M. S. & Boyle, P. R. 1985. A new application of serological techniques to gut content analysis. Journal of Experimental Marine Biology and Ecology, 90, 19.
Hartwick, B., Tulloch, L. & Macdonald, S., 1981. Feeding and growth of Octopus dofleini (Wülker). Veliger, 24, 129138.
Hirtle, R. W. M., 1978. Observations on feeding and grooming behaviour of captive squid, Illex illecebrosus (Lesueur, 1821). Technical Report. Fisheries and Marine Service, Canada, no. 833, 13.1–13.5.
Hurley, A. C, 1976. Feeding behavior, food consumption, growth and respiration of the squid Loligo opalescens raised in the laboratory. Fishery Bulletin. National Oceanic and Atmospheric Administration of the United States, 74, 176182.
Laurell, C.-B., 1966. Quantitative estimation of proteins by electrophoresis in agarose gel, containing antibodies. Analytical Biochemistry, 15, 4552.
Maldonado, H., 1963. The positive learning process in Octopus vulgaris. Zeitschrift fur vergleichende Physiologie, 47, 191214.
Martins, H. R., 1982. Biological studies of the exploited stock of Loligo forbesi (Mollusca: Cephalopoda) in the Azores. Journal of the Marine Biological Association of the United Kingdom, 62, 799808.
Messenger, J. B., 1968. The visual attack of the cuttlefish Sepia officinalis. Animal Behaviour, 16, 342357.
Moriyasu, M. 1981. Biologie des Pêches de Céphalopodes Benthiques. Application aux Eledones, Eledone cirrhosa (Lam., 1798) du Golfe du Lion. Thèse Doctorat, 3ème cycle, Université des Sciences et Techniques du Languedoc, France.
Moynihan, M. & Rodaniche, A. F., 1982. The Behaviour and Natural History of the Caribbean Reef Squid Sepioteuthis sepioidea. Berlin: Verlag Paul Parey. [Advances in Ethology, no. 25; Journal of Comparative Ethology, supplement 25.]
Nigmatullin, C. M. & Ostapenko, A. A. 1976. Feeding of Octopus vulgaris Lam. from the northwest African coast. International Council for the Exploration of the Sea (CM. Papers and Reports), K:6, 15 pp. [Mimeo.]
Nixon, M., 1984. Is there external digestion in Octopus? Journal of Zoology, 202, 441447.
Nixon, M., 1986. Cephalopod diets. In CephalopodLife Cycles, vol. 2 (ed. Boyle, P. R.), pp. 201219. London: Academic Press.
Sanchez, P., 1981. Régime alimentaire d 'Eledone cirrosa (Lamarck, 1798) (Mollusca: Cephalopoda) dans la Mer Catalane. Rapport et proces-verbaux des reunions. Commission internationale pour I'exploration scientifique de la Mer Méditerranée, 27(5), 209212.
Vovk, A. N., 1983. Food Relations of Long-finned Squid, Loligo pealei Lesueur, in the Northwest Atlantic and its Position in the Ecosystem. North Atlantic Fisheries Organisation. [SCR Document, 83/IX/83.]
Yarnall, J. L., 1969. Aspects of the behaviour of Octopus cyanea Gray. Animal Behaviour, 17, 747754.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Journal of the Marine Biological Association of the United Kingdom
  • ISSN: 0025-3154
  • EISSN: 1469-7769
  • URL: /core/journals/journal-of-the-marine-biological-association-of-the-united-kingdom
Please enter your name
Please enter a valid email address
Who would you like to send this to? *


Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed