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Genetic Divergence Between South African Helcion Species and North-East Atlantic H. Pellucidum (Mollusca: Patellogastropoda)

  • L.I. Weber (a1), D.R. Gray (a1), A.N. Hodgson (a1) and S.J. Hawkins (a1)

Allozyme electrophoresis was used to estimate the genetic divergence between three species of South African Helcion and the north-east Atlantic H. pellucidum (Patellidae). At a finer level the genetic relationships among South African Helcion were explored. Twenty-one loci were resolved for all the species. High values of expected heterozygosities (0·202–0·341) were found for the genus. Phenetic analysis (UPGMA (unweighted pair-group method with artihmetic averaging) and Distance Wagner Procedure) and cladistic parsimony methods (alleles as binary characters and loci as multistate unordered characters) agreed in showing the same topology for the tree which represents the genetic relationships among Helcion species. The north-east Atlantic H. pellucidum was the most divergent species showing high values of Nei's genetic distances (1·423–1·654) with its South African congeners. In the literature such values of genetic divergence have been associated with non-congeneric species. The degree of genetic divergence (0·351–0·615) found within the South African branch was characteristic for congeneric species. The results of this study supported the monophyletic condition of this branch, and showed H. pectunculus and H. pruinosus to be more closely related to each other than to H. dunkeri. Nevertheless, the monophyly of the genus Helcion is uncertain and therefore its taxonomic status was questioned. A possible independent origin for northern and southern hemisphere species of Helcion is considered.

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Avise J.C., 1994. Molecular markers, natural history and evolution. New York: Chapman & Hall.
Ayala F.J., Tracey M.L., Hedgecock D. & Richmond R.C., 1974. Genetic differentiation during the speciation process in Drosophila. Evolution, 28, 576592.
Brewer G.J., 1970. An introduction of isozymes techniques. New York: Academic Press.
Buth D.G., 1984. The application of electrophoretic data in systematic studies. Annual Review of Ecology and Systematics, 15, 501522.
Côrte-Real H.B.S.M., 1992. Taxonomy and population genetics of exploited species of Patella in the Azores, Madeira and Canaries. PhD thesis, University of Liverpool.
Cretella M., Scillitani G., Toscano F., Turella P., Picariello O. & Cataudo A., 1994. Relationships between Patella ferruginea Gmelin, 1791 and the other Tyrrhenian species of Patella (Gastropoda: Patellidae). Journal of Molluscan Studies, 60, 917.
D'angelo G. & Gargiullo S., 1979. Guida alle conchiglie Mediterranee. Milano: Fabbri Editori, S.P.A.
Ebling F.J., Kitching J.A., Purchon R.D. & Bassindale R., 1948. The ecology of Lough Ine rapids with special reference to water currents. 2. The fauna of the Sacchorhiza Canopy. Journal of Animal Ecology, 17, 233244.
Fretter V. & Graham A., 1976. The prosobranch molluscs of Britain and Denmark. Part 1. Pleurotomariacea, Fissurellacea and Patellacea. Journal of Molluscan Studies, supplement 1, 37 pp.
Gaffney P.M., 1980. On the number of Patella species in south-west England. Journal of the Marine Biological Association of the United Kingdom, 60, 565574.
Gillespie J.H., 1991. The causes of molecular evolution. Oxford University Press.
Graham A. & Fretter V., 1947. The life history of Patina pellucida (L.). Journal of the Marine Biological Association of the United Kingdom, 26, 509601.
Harris H. & Hopkinson D.A., 1976. Handbook of enzyme electrophoresis in human genetics. Amsterdam: North Holland.
Hayward P.J. & Ryland J.S., 1990. The marine fauna of the British Isles and north-west Europe. Vol. 2. Molluscs to chordates. Oxford: Clarendon Press.
Hendy M.D. & Penny D., 1982. Branch and bound algorithms to determine minimal evolutionary trees. Mathematical Biosciences, 59, 277290.
Hillis D.M. & Moritz C., 1990. Molecular systematics. Sunderland, Massachusetts: Sinauer Associates.
Hodgson A.N. & Bernard R.T.F., 1988. A comparison of the structure of the spermatozoa and spermatogenesis of 16 species of patellid limpet (Mollusca: Gastropoda: Archaeogastropoda). Journal of Morphology, 195, 205223.
Hodgson A.N., Ridgway S.A., Branch G.M. & Hawkins S.J., 1996. Spermatozoan morphology of 19 species of prosobranch limpets (Patellogastropoda) with a discussion of patellid relationships. Philosophical Transactions of the Royal Society B, 357, 339347.
Hurst C.D. & Skibinski D.O.F., 1995. Comparison of allozyme and mitochondrial DNA spatial differentiation in the limpet Patella vulgata. Marine Biology, 122, 257263.
IUBNC (International Union of Biochemistry, Nomenclature Committee), 1984. Enzyme nomenclature. Orlando, Florida: Academic Press.
Jamieson B.G.M., Hodgson A.N. & Bernard R.T.F., 1991. Phylogenetic trends and variation in the ultrastructure of the spermatozoa of sympatric species of South African patellid limpets (Archaeogastropoda, Mollusca). Invertebrate Reproduction and Development, 20, 137146.
Kain J.M. & Svendsen P., 1969. A note on the behaviour of Patina pellucida in Britain and Norway. Sarsia, 38, 2530.
Kilburn R. & Rippey E., 1982. Sea shells of Southern Africa. Johannesburg: Macmillan South Africa.
Li W.-H. & Grauer D., 1991. Fundamentals of molecular evolution. Sunderland, Massachusetts: Sinauer Associates.
Lindberg D.R., 1988. The Patellogastropoda. Malacological Review, supplement 4, 3563.
Lindberg D.R. & McLean J.H., 1981. Tropical eastern Pacific limpets of the family Acmaeidae (Mollusca: Archaeogastropoda): generic criteria and descriptions of six new species from the mainland and the Galapagos Islands. Proceedings of the California Academy of Sciences, 42(12), 323339.
Martin A.P. & Palumbi S.R., 1993. Body size, metabolic rate, generation time, and the molecular clock. Proceedings of the National Academy of Sciences of the United States of America, 90, 40874091.
McGrath D., 1992. Recruitment and growth of the blue-rayed limpet, Helcion pellucidum (L.), in south east Ireland. Journal of Molluscan Studies, 58, 425431.
Murphy R.W., Sites J.W., Buth D.G. & Haufler C.H., 1990. Proteins. I. Isozyme electrophoresis. In Molecular systematics (ed. D.M. Hillis and C. Moritz), pp. 45126. Sunderland, Massachusetts: Sinauer Associates.
Nei M., 1978. Estimation of average heterozygosity and genetic distance from small number of individuals. Genetics, 89, 583590.
Nei M., 1987. Molecular evolutionary genetics. New York: Columbia University Press.
Poulik M.D., 1957. Starch gel electrophoresis in a discontinuous system of buffers. Nature, London, 180, 14771479.
Powell A.W.B., 1973. The patellid limpets of the world (Patellidae). Indo-Pacific Mollusca, 3(15), 75206.
Redfield J.A. & Salini J.P., 1980. Techniques of starch-gel electrophoresis of penaeid prawn enzymes (Penaeus spp. and Metapenaeus spp.). Report. Division of Fisheries and Oceanography, CSIRO, Australia, no. 116, 20 pp.
Richardson B.J., Baverstock P.R. & Adams M., 1986. Allozyme electrophoresis. Sydney: Academic Press.
Ridgway S.A., 1994. The systematics, phylogeny and biogeography of the limpets genus Patella (Mollusca: Gastropoda) in the northern Atlantic Ocean and Mediterranean Sea. PhD thesis, University of London.
Sella G. & Badino G., 1980. Differenze elettroforetiche tra le specie mediterranee di Patella e loro impiego nela tassonomia del genere. In Atti IV Congresso S.M.I, (ed. F. Giusti), pp. 357368. Sienna: Atti Acadelma Fisiocritici.
Sella G., Robotti C.A. & Biglione V., 1993. Genetic divergence among three sympatric species of Mediterranean Patella (Archaeogastropoda). Marine Biology, 115, 401405.
Shaklee J.B., Allendorf F.W., Morizot D.C. & Whitt G.S., 1990. Gene nomenclature for protein-coding loci in fish Transactions of the American Fisheries Society, 119, 215.
Shaw S.R. & Prasad R., 1970. Starch gel electrophoresis of enzymes – a compilation of recipes. Biochemical Genetics, 4, 297320.
Siciliano M.J. & Shaw C.R., 1976. Separation and visualisation of enzymes of gels. In Chromatographic and electrophoretic techniques. Vol. 2. Zone electrophoresis (ed. I. Smith), pp. 185209. London: Heinemann Medical Books.
Sousa M. & Oliveira E., 1994. An ultrastructural study of spermatogenesis in Helcion pellucidus (Gastropoda, Prosobranchia). Invertebrate Reproduction and Development, 26, 119126.
Swofford D.L., 1985. PAUP. Phylogenetic analysis using parsimony. Version 2·4. Illinois: Illinois Natural History Survey.
Swofford D.L. & Selander R.B., 1981. BIOSYS-I. A fortran program for the comprehensive analysis of electrophoretic data in population genetics and systematics. Journal of Heredity, 72, 281283.
Thorpe J.P., 1982. The molecular clock hypothesis: biochemical evolution, genetic differentiation and systematics. Annual Review of Ecology and Systematics, 13, 139168.
Thorpe J.P., 1989. Possible effects of interprotein variation in mean rate of amino acid substitution on the relationship of genetic distance with time since evolutionary divergence. Biological Journal of the Linnean Society, 37, 335344.
Thorpe J.P. & Solé-Cava A.M., 1994. The use of allozyme electrophoresis in invertebrate systematics. Zoologica Scripta, 23, 318.
Thorson G., 1941. Marine Gastropoda Prosobranchiata. Zoology of Iceland, Copenhagen, 4(60), 1150.
Vahl O., 1971. Growth and density of Patina pellucida (L.) (Gastropoda: Prosobranchia) on Laminaria hyperborea (Gunnerus) from western Norway. Ophelia, 9, 3150.
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Journal of the Marine Biological Association of the United Kingdom
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  • EISSN: 1469-7769
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