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Simultaneous detection of Aurelia and Chrysaora scyphozoan jellyfish on a DNA microarray

Published online by Cambridge University Press:  19 August 2009

Jang-Seu Ki ,
Dae-Sik Hwang  and
Jae-Seong Lee
Jang-Seu Ki
Affiliation:
Department of Molecular and Environmental Bioscience, Graduate School, Hanyang University, Seoul 133–791, South Korea National Research Laboratory of Marine Molecular and Environmental Bioscience, Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 133–791, South Korea
Dae-Sik Hwang
Affiliation:
Department of Molecular and Environmental Bioscience, Graduate School, Hanyang University, Seoul 133–791, South Korea
Jae-Seong Lee*
Affiliation:
National Research Laboratory of Marine Molecular and Environmental Bioscience, Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 133–791, South Korea
*
Correspondence should be addressed to: J.-S. Lee, Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 133–791, South Korea email: jslee2@hanyang.ac.kr
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Abstract

To demonstrate the effectiveness of microarrays for the detection of jellyfish, we developed a low density DNA chip based on the mitochondrial COI gene sequences of scyphozoans (jellyfish). We designed species-specific oligonucleotide probes by sequence comparisons between scyphozoans and other cnidarians such as hydrozoans and anthozoans. Each amine-labelled capture probe was arrayed onto a silylated slide. PCR products of the COI gene were hybridized to the DNA microarray that contained COI consensus sequences. We tested the ability of the DNA chip to discriminate between species from the genera Aurelia and Chrysaora based on samples of both species from the polyp and ephyra stages. The array produced unique hybridization patterns for each of the two tested jellyfish species. Furthermore, we were able to simultaneously detect individual jellyfish species from mixtures of these two different species in the laboratory and from environmental samples. These results show that the low density DNA chip that we designed can be used as a technical platform for parallel molecular detection of various jellyfish species.

Keywords

jellyfishmitochondrial COIoligonucleotide arrayDNAchipparallel detection
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 90 , Special Issue 6: In Honour of Sir Frederick Stratten Russell, FRS , September 2010 , pp. 1111 - 1117
Copyright
Copyright © Marine Biological Association of the United Kingdom 2009

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