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Extraction of DNA from lichen-forming and lichenicolous fungi: a low-cost fast protocol using Chelex

Published online by Cambridge University Press:  19 September 2017

Zuzana Ferencova ,
Víctor J. Rico  and
David L. Hawksworth
Zuzana Ferencova
Affiliation:
Departamento de Biología Vegetal II, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ramón y Cajal, Madrid 28040, Spain. Email: zferenc@farm.ucm.es
Víctor J. Rico
Affiliation:
Departamento de Biología Vegetal II, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ramón y Cajal, Madrid 28040, Spain. Email: zferenc@farm.ucm.es
David L. Hawksworth
Affiliation:
Comparative Plant and Fungal Biology, Royal Botanic Gardens, Kew, Surrey TW9 3DS, UK; also Department of Life Sciences, The Natural History Museum, Cromwell Road, London SW7 5BD, UK
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Type
Short Communications
Information
The Lichenologist , Volume 49 , Issue 5 , September 2017 , pp. 521 - 525
Copyright
© British Lichen Society, 2017 

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References

Casquet, J., Thebaud, C. & Gillespie, R. G. (2012) Chelex without boiling, a rapid and easy technique to obtain stable amplifiable DNA from small amounts of ethanol‐stored spiders. Molecular Ecology Resources 12: 136–141.CrossRefGoogle ScholarPubMed
Cubero, O. F., Crespo, A., Fatehi, J. & Bridge, P. D. (1999) DNA extraction and PCR amplification method suitable for fresh, herbarium-stored, lichenized, and other fungi. Plant Systematics and Evolution 216: 243–249.CrossRefGoogle Scholar
Eckhart, L., Bach, J., Ban, J. & Tschachler, E. (2000) Melanin binds reversibly to thermostable DNA polymerase and inhibits its activity. Biochemical and Biophysical Research Communications 271: 726–730.CrossRefGoogle ScholarPubMed
Ertz, D., Lawrey, J. D., Common, R. S. & Diederich, P. (2014) Molecular data resolve a new order of Arthoniomycetes sister to the primarily lichenized Arthoniales and composed of black yeasts, lichenicolous and rock-inhabiting species. Fungal Diversity 66: 113–137.CrossRefGoogle Scholar
Gardes, M. & Bruns, T. D. (1993) ITS primers with enhanced specificity for basidiomycetes – application to the identification of mycorrhizae and rusts. Molecular Ecology 2: 113–118.CrossRefGoogle Scholar
Grube, M., DePriest, P. T., Gargas, A. & Hafellner, J. (1995) DNA isolation from lichen ascomata. Mycological Research 99: 1321–1324.CrossRefGoogle Scholar
Gueidan, C., Aptroot, A., Cáceres, M. E. S. & Binh, N. Q. (2016) Molecular phylogeny of the tropical lichen family Pyrenulaceae: contribution from dried herbarium specimens and FTA card samples. Mycological Progress 15: 1–21.Google Scholar
Hall, T. A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.Google Scholar
HwangBo, K., Son, S. H., Lee, J. S., Min, S. R., Ko, S. M., Liu, J. R. & Jeong, W. J. (2010) Rapid and simple method for DNA extraction from plant and algal species suitable for PCR amplification using a chelating resin Chelex 100. Plant Biotechnology Reports 4: 49–52.CrossRefGoogle Scholar
Lawrey, J. D., Binder, M., Diederich, P., Molina, M. C., Sikaroodi, M. & Ertz, D. (2007) Phylogenetic diversity of lichen-associated homobasidiomycetes. Molecular Phylogenetics and Evolution 44: 778–789.CrossRefGoogle ScholarPubMed
Lohtander, K., Myllys, L., Sundin, R., Källersjö, M. & Tehler, A. (1998) The species pair concept in the lichen Dendrographa leucophaea (Arthoniales): analyses based on ITS sequences. Bryologist 101: 404–411.CrossRefGoogle Scholar
Myllys, L., Lohtander, K., Källersjö, M. & Tehler, A. (1999) Sequence insertions and ITS data provide congruent information on Roccella canariensis and R. tuberculata (Arthoniales, Euascomycetes) phylogeny. Molecular Phylogenetics and Evolution 12: 295–309.CrossRefGoogle Scholar
Pedersen, N., Russell, S. J., Newton, A. E. & Ansell, S. W. (2006) A novel molecular protocol for the rapid extraction of DNA from bryophytes and the utility of direct amplification of DNA from a single dwarf male. Bryologist 109: 257–264.CrossRefGoogle Scholar
Rehner, S. A. & Samuels, G. J. (1994) Taxonomy and phylogeny of Gliocladium analysed from nuclear large subunit ribosomal DNA sequences. Mycological Research 98: 625–634.CrossRefGoogle Scholar
Schrader, C., Schielke, A., Ellerbroek, L. & Johne, R. (2012) PCR inhibitors – occurrence, properties and removal. Journal of Applied Microbiology 113: 1014–1026.CrossRefGoogle ScholarPubMed
Singer-Sam, J., Tanguay, R. L. & Riggs, A. O. (1989) Use of Chelex to improve PCR signal from a small number of cells. Amplifications 3: 11. [Not seen.]Google Scholar
Strange, J. P., Knoblett, J. & Griswold, T. (2009) DNA amplification from pin-mounted bumble bees (Bombus) in a museum collection: effects of fragment size and specimen age on successful PCR. Apidologie 40: 134–139.CrossRefGoogle Scholar
Tebbe, C. C. & Vahjen, W. (1993) Interference of humic acids and DNA extracted directly from soil in detection and transformation of recombinant DNA from bacteria and a yeast. Applied and Environmental Microbiology 59: 2657–2665.CrossRefGoogle ScholarPubMed
Tsai, Y. L. & Olson, B. H. (1992) Rapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reaction. Applied and Environmental Microbiology 58: 2292–2295.CrossRefGoogle ScholarPubMed
Turan, C., Nanni, I. M., Brunelli, A. & Collina, M. (2015) New rapid DNA extraction method with Chelex from Venturia inaequalis spores. Journal of Microbiological Methods 115: 139–143.CrossRefGoogle ScholarPubMed
Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246.CrossRefGoogle ScholarPubMed
Walsh, P. S., Metzger, D. A. & Higuchi, R. (1991) Chelex®100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10: 506–513.Google Scholar
White, T. J., Bruns, T., Lee, S. & Taylor, J. W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: A Guide to Methods and Applications (M. A. Innis, D. H. Gelfand, J. J. Sninsky & T. J. White, eds): 315–322. San Diego: Academic Press.Google Scholar
Wolinski, H., Grube, M. & Blanz, P. (1999) Direct PCR of symbiotic fungi using microslides. Biotechniques 26: 454–455.CrossRefGoogle ScholarPubMed
Zhang, Y. J., Zhang, S., Liu, X. Z., Wen, H. A. & Wang, M. (2010) A simple method of genomic DNA extraction suitable for analysis of bulk fungal strains. Letters in Applied Microbiology 51: 114–118.Google ScholarPubMed