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The genus Relicinopsis is nested within Relicina (Parmeliaceae, Ascomycota)

  • Paul M. KIRIKA (a1) (a2), Pradeep K. DIVAKAR (a3), Steven D. LEAVITT (a4), Kawinnat BUARUANG (a5), Ana CRESPO (a3), George MUGAMBI (a6), Grace W. GATHERI (a1) and H. Thorsten LUMBSCH (a4)...
Abstract

Macro-morphological features traditionally used to segregate genera in Parmeliaceae have been shown to be highly plastic, placing limits on their taxonomic value. Here we aim to elucidate the evolutionary relationships of the genera Relicina and Relicinopsis and reassess the phenotypic features traditionally used to separate these genera. To this end, we gathered ribosomal DNA sequences of ITS, nuLSU and mtSSU and analyzed them in a phylogenetic framework. Relicina was recovered as paraphyletic, with Relicinopsis nested within, and three different clades were identified within Relicina. Alternative hypothesis tests significantly rejected the monophyly of Relicina. Our results indicate that the presence or absence of bulbate cilia is of limited taxonomic value in this clade. Based on differences in conidia, however, we propose to accept Relicinopsis as a subgenus within Relicina as Relicina subgen. Relicinopsis (Elix & Verdon) Kirika, Divakar & Lumbsch. It is proposed that five new combinations of species previously classified in Relicinopsis be placed in Relicina.

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Corresponding author
Email: tlumbsch@fieldmuseum.org
References
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Arup, U., Ekman, S., Lindblom, L. & Mattsson, J. E. (1993) High performance thin layer chromatography (HPTLC), an improved technique for screening lichen substances. Lichenologist 25: 6171.
Biomatters Ltd (2005–2015) Geneious r8. Available at: http://www.geneious.com/previous-versions
Buaruang, K., Scharnagl, K., Divakar, P. K., Leavitt, S. D., Crespo, A., Nash, T. H., Manoch, L., Lücking, R. & Lumbsch, H. T. (2015) Molecular data support Pseudoparmelia as a distinct lineage related to Relicina and Relicinopsis (Ascomycota, Lecanorales). Lichenologist 47: 4349.
Crespo, A., Kauff, F., Divakar, P. K., Amo, G., Arguello, A., Blanco, O., Roca-Valiente, B., Núñez-Zapata, J., Cubas, P., Argüello, A. et. al. (2010) Phylogenetic generic classification of parmelioid lichens (Parmeliaceae, Ascomycota) based on molecular, morphological and chemical evidence. Taxon 59: 17351753.
Crespo, A., Divakar, P. K. & Hawksworth, D. L. (2011) Generic concepts in parmelioid lichens, and the phylogenetic value of characters used in their circumscription. Lichenologist 43: 511535.
Divakar, P. K. & Crespo, A. (2015) Molecular phylogenetic and phylogenomic approaches in studies of lichen systematics and evolution. In Recent Advances in Lichenology: Modern Methods and Approaches in Lichen Systematics and Culture Techniques, Volume 2 (D. K. Upreti, P. K. Divakar, V. Shukla & R. Bajpai, eds): 4560. New Delhi: Springer India.
Divakar, P. K. & Upreti, D. K. (2005) Parmelioid Lichens in India (A Revisionary Study). Dehra Dun: Bishen Singh Mahendra Pal Singh.
Divakar, P. K., Ferencova, Z., Del Prado, R., Lumbsch, H. T. & Crespo, A. (2010) Remototrachyna, a new tropical lineage in hypotrachynoid lichens (Parmeliaceae, Ascomycota): a multigene and morphological approach. American Journal of Botany 97: 579590.
Divakar, P. K., Crespo, A., Núñez-Zapata, J., Flakus, A., Sipman, H. J. M., Elix, J. A. & Lumbsch, H. T. (2013) A molecular perspective on generic concepts in the Hypotrachyna clade (Parmeliaceae, Ascomycota). Phytotaxa 132: 2138.
Divakar, P. K., Crespo, A., Wedin, M., Leavitt, S. D., Hawksworth, D. L., Myllys, L., McCune, B., Randlane, T., Bjerke, J. W., Ohmura, Y. et al. (2015) Evolution of complex symbiotic relationships in a morphologically derived family of lichen-forming fungi. New Phytologist 208: 12171226.
Drummond, A. & Rambaut, A. (2007) BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evolutionary Biology 7: 214.
Drummond, A. J., Suchard, M. A., Xie, D. & Rambaut, A. (2012) Bayesian phylogenetics with BEAUti and the BEAST 1.7. Molecular Biology and Evolution 29: 19691973.
Elix, J. A. (1993) Progress in the generic delimitation of Parmelia sensu lato lichens (Ascomycotina: Parmeliaceae) and a synoptic key to the Parmeliaceae . Bryologist 96: 359383.
Elix, J. A. (1994) Parmeliaceae . Flora of Australia 55: 1360.
Elix, J. A., Johnston, J. & Verdon, D. (1986) Canoparmelia, Paraparmelia and Relicinopsis, three new genera in the Parmeliaceae (lichenized Ascomycotina). Mycotaxon 27: 271282.
Gardes, M. & Bruns, T. D. (1993) ITS primers with enhanced specificity for basidiomycetes – application to the identification of mycorrhizae and rusts. Molecular Ecology 2: 113118.
Gutierrez, G., Blanco, O., Divakar, P. K., Lumbsch, H. T. & Crespo, A. (2007) Patterns of group I intron presence in nuclear SSU rDNA of the lichen family Parmeliaceae . Journal of Molecular Evolution 64: 181195.
Hale, M. E. (1974) Bulbothrix, Parmelina, Relicina, and Xanthoparmelia, four new genera in the Parmeliaceae . Phytologia 28: 479490.
Hale, M. E. (1975) A monograph of the lichen genus Relicina (Parmeliaceae). Smithsonian Contributions to Botany 26: 132.
Hale, M. E. (1976) A monograph of the lichen genus Pseudoparmelia Lynge (Parmeliaceae). Smithsonian Contributions to Botany 31: 162.
Hörandl, E. & Stuessy, T. F. (2010) Paraphyletic groups as natural units of biological classification. Taxon 59: 16411653.
Katoh, K. & Toh, H. (2008) Recent developments in the MAFFT multiple sequence alignment program. Briefings in Bioinformatics 9: 286298.
Katoh, K., Kuma, K. I., Toh, H. & Miyata, T. (2005) MAFFT version 5: improvement in accuracy of multiple sequence alignment. Nucleic Acids Research 33: 511518.
Lanfear, R., Calcott, B., Ho, S. Y. & Guindon, S. (2012) PartitionFinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molecular Biology and Evolution 29: 16951701.
Larena, I., Salazar, O., González, V., Julián, M. C. & Rubio, V. (1999) Design of a primer for ribosomal DNA internal transcribed spacer with enhanced specificity for ascomycetes. Journal of Biotechnology 75: 187194.
Lumbsch, H. T. (2001) Analysis of phenolic products in lichens for identification and taxonomy. In Protocols in Lichenology: Culturing, Biochemistry, Ecophysiology and Use in Biomonitoring (I. Kranner, R. Beckett & A. Varma, eds): 281295. Berlin: Springer.
Lumbsch, H. T. (2007) Recent trends in phylogeny and classification of lichen-forming ascomycetes. In Fungi: Multifaceted Microbes (B. N. Ganguli & S. K. Deshmukh, eds): 153168. Delhi: Anamaya.
Printzen, C. (2010) Lichen systematics: the role of morphological and molecular data to reconstruct phylogenetic relationships. Progress in Botany 71: 233279.
Rambaut, A. & Drummond, A. J. (2009) Tracer version 1.5. Available at: http://beast.bio.ed.ac.uk.
Schmidt, H. A., Strimmer, K., Vingron, M. & von Haeseler, A. (2002) TREE-PUZZLE: maximum likelihood phylogenetic analysis using quartets and parallel computing. Bioinformatics 18: 502504.
Shimodaira, H. & Hasegawa, M. (1999) Multiple comparisons of log-likelihoods with applications to phylogenetic inference. Molecular Biology and Evolution 16: 11141116.
Stamatakis, A. (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22: 26882690.
Stamatakis, A., Hoover, P. & Rougemont, J. (2008) A rapid bootstrap algorithm for the RAxML Web servers. Systematic Biology 57: 758771.
Strimmer, K. & Rambaut, A. (2002) Inferring confidence sets of possibly misspecified gene trees. Proceedings of the Royal Society of London Series B-Biological Sciences 269: 137142.
Swinscow, T. D. V. & Krog, H. (1988) Macrolichens of East Africa. London: British Museum (Natural History).
Talavera, G. & Castresana, J. (2007) Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. Systematic Biology 56: 564577.
Thell, A., Crespo, A., Divakar, P. K., Kärnefelt, I., Leavitt, S. D., Lumbsch, H. T. & Seaward, M. R. D. (2012) A review of the lichen family Parmeliaceae – history, phylogeny and current taxonomy. Nordic Journal of Botany 30: 641664.
Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 42384246.
White, T. J., Bruns, T. D., Lee, S. & Taylor, J. (eds) (1990) Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics. San Diego: Academic Press.
Wiens, J. J. (1998) Combining data sets with different phylogenetic histories. Systematic Biology 47: 568581.
Zoller, S., Scheidegger, C. & Sperisen, C. (1999) PCR primers for the amplification of mitochondrial small subunit ribosomal DNA of lichen-forming ascomycetes. Lichenologist 31: 511516.
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