Skip to main content
×
×
Home

A hidden basidiolichen rediscovered: Omphalina oreades is a separate species in the genus Lichenomphalia (Basidiomycota: Agaricales: Hygrophoraceae)

  • Robert LÜCKING (a1), R. Greg THORN (a2), Irja SAAR (a3), Michele D. PIERCEY-NORMORE (a4), Bibiana MONCADA (a5), Jennifer DOERING (a6), Henry MANN (a4), Renée LEBEUF (a7), Maria VOITK (a8) and Andrus VOITK (a8)...
Abstract

Molecular studies have shown the type collection of Omphalina oreades to be conspecific with a small brown basidiolichen from the Appalachian range in Newfoundland, both with 4-spored basidia. Two sequences deposited in GenBank, originally identified as O. grisella, fell in the same clade. Sequences of the type collection of Omphalia grisella, with 2-spored basidia, formed a sister clade together with two GenBank deposits, one identified as O. grisella and the other as Omphalina velutina. Omphalina oreades is recombined here as Lichenomphalia oreades comb. nov., and the species redescribed and illustrated. Sequences of the internal transcribed spacer regions of nuclear ribosomal DNA (ITS rDNA) from the algae associated with two collections of L. oreades fell within a highly supported clade with members of an undetermined species of Coccomyxa. The most abundant algal ribosomal large subunit sequence from granules at the base of a different collection matched GenBank sequences identified as Chloroidium ellipsoideum, which is probably either a free-living algal species or a partner to a species of Trapeliopsis. The second most abundant sequence matched Coccomyxa subellipsoidea and is the most likely photobiont of L. oreades. Further studies are required to elucidate the relationship of L. velutina to these taxa.

Copyright
Corresponding author
Email: seened@gmail.com
References
Hide All
Asemaninejad, A., Weerasuriya, N., Gloor, G. B., Lindo, Z. & Thorn, R. G. (2016) New primers for discovering fungal diversity using nuclear large ribosomal DNA. PLoS ONE 11: e0159043.
Barrasa, J. M. & Rico, V. J. (2001) Lichenized species of Omphalina (Tricholomataceae) in the Iberian Peninsula. Lichenologist 33: 371386.
Beiggi, S. & Piercey-Normore, M. D. (2007) Evolution of ITS ribosomal RNA secondary structures in fungal and algal symbionts of selected species of Cladonia sect. Cladonia (Cladoniaceae, Ascomycotina). Journal of Molecular Evolution 64: 528542.
Bigelow, H. E. (1968) Contribution to the fungus flora of northeastern North America. IV. Rhodora 68: 175191.
Bigelow, H. E. (1983) Some clampless species of Clitocybe . Cryptogamie, Mycologie 4: 9398.
Bigelow, H. E. (1985) North American species of Clitocybe. II. Beihefte zur Nova Hedwigia 81: 281471.
Clémençon, H. (1982) Kompendium der Blätterpilze. Europäische omphalinoide Tricholomaceae. Zeitschrift für Mykologie 48: 195237.
Consortium of Pacific Northwest Herbaria (2016) URL http://www.pnwherbaria.org/. (Accessed 2 March 2017).
Courtecuisse, R. (2008) Novitates. 5. Nouvelles combinaisons et nouveaux noms nécessaires suite à la mise au point du référentiel des noms de champignons présents sur le territoire national métropolitain (1 - Basidiomycètes). Documents Mycologiques 34 (135–136):4852.
Contu, M. & La Rocca, S. (1999) Funghi della zona mediterranea insulare italiana. Funghi non delineati 9: 148.
Darienko, T., Gustavs, L., Mudimu, O., Rad Menendez, C., Schumann, R., Karsten, U., Friedl, T. & Pröschold, T. (2010) Chloroidium, a common terrestrial coccoid green alga previously assigned to Chlorella (Trebouxiophyceae, Chlorophyta). European Journal of Phycology 45: 7995.
Doering, M. & Piercey-Normore, M. D. (2009) Genetically divergent algae shape a lichen community on Jack Pine in Manitoba. Lichenologist 41: 6980.
Elborne, S. A. (2008) Lichenomphalia . In Funga Nordica (H. Knudsen & J. Vesterholt, eds): 913. København: Nordsvamp.
Elborne, S. A. (2012) Lichenomphalia . In Funga Nordica (H. Knudsen & J. Vesterholt, eds): 293295. København: Nordsvamp.
Favre, J. (1955) Les champignons supérieurs de la zone alpine du Parc National suisse. Ergebnisse der Wissenschaftlichen Untersuchungen des Schweizerischen Nationalparks 5: 1212.
Geml, J., Timling, I., Robinson, C. H., Lennon, N., Nusbaum, H. C., Brochmann, C., Noordeloos, M. E. & Taylor, D. L. (2012) An arctic community of symbiotic fungi assembled by long-distance dispersers: phylogenetic diversity of ectomycorrhizal basidiomycetes in Svalbard based on soil and sporocarp DNA. Journal of Biogeography 39: 7488.
Grube, M., DePriest, P. T., Gargas, A. & Hafellner, J. (1995) DNA isolation from lichen ascomata. Mycological Research 99: 13211324.
Hall, T. A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 9598.
Hodkinson, B., Moncada, B. & Lücking, R. (2013) Lepidostromatales ordo novum, a unique, lichenized lineage within Basidiomycota (Agaricomycetes), with the description of two new genera, Ertzia and Sulzbacheromyces, and one new species, Lepidostroma winklerianum . Fungal Diversity 64: 165179.
Horak, E. (1960) Die Pilzvegetation im Gletschervorfeld (2290–2350m) des Rotmoosferners in den Ötztaler Alpen. Nova Hedwigia 2: 487507.
Index Fungorum Partnership (2016) URL www.indexfungorum.org. (Accessed 2 March 2017).
Jørgensen, P. M. & Ryman, S. (1989) The correct names of the lichenized Omphalina species in Scandinavia. Graphis Scripta 3: 138143.
Karsten, P. A. (1889) Symbolae ad mycologiam fennicam, pars XXIX. Meddelanden af Societas pro fauna et flora Fennica 16: 84106.
Katoh, K., Asimenos, G. & Toh, H. (2009) Multiple alignment of DNA sequences with MAFFT. Methods in Molecular Biology 537: 3964.
Kuyper, T. W. (1986) Generic delimitation in European omphalinoid Tricholomataceae . Atti del Centro Studi per la Flora Mediterranea (Borgo Val di Taro, Italy) 6: 83104.
Kuyper, T. W. (1995) Genus Phytoconis . In Flora Agaricina Neerlandica Vol. 3 (C. Bas, T. W. Kuyper, M. E. Noordeloos & E. C. Vellinga, eds): 8992. Rotterdam: A. A. Balkema.
Lodge, J. D., Mahajabeen, P., Matheny, P. B., Aime, M. C., Cantrell, S. A., Boertmann, D., Kovalenko, A., Vizzini, A., Dentinger, B. T. M., Kirk, P. M. et al. (2014) Molecular phylogeny, morphology, pigment chemistry and ecology in Hygrophoraceae (Agaricales). Fungal Diversity 64: 199.
Lohtander, K., Oksanen, I. & Rikkinen, J. (2003) Genetic diversity of green algal and cyanobacterial photobionts in Nephroma (Peltigerales). Lichenologist 35: 325339.
Lumbsch, T. & Schmitt, I. (2001) Molecular data suggest that the lichen genus Pertusaria is not monophyletic. Lichenologist 33: 161170.
Lutzoni, F. M. (1997) Phylogeny of lichen- and non-lichen-forming omphalinoid mushrooms and the utility of testing for combinability among multiple data sets. Systematic Biology 46: 373406.
Malavasi, V., Škaloud, P., Rindi, F., Tempesta, S., Paoletti, M. & Pasqualetti, M. (2016) DNA-based taxonomy in ecologically versatile microalgae: a re-evaluation of the species concept within the coccoid green algal genus Coccomyxa (Trebouxiophyceae, Chlorophyta). PLoS ONE 11: e0151137.
Mycology Collections Portal (2016) URL http://mycoportal.org/. (Accessed 2 March 2017).
Neville, P. & Fouchier, F. (2009) Une nouvelle espèce méditerranéenne de Lichenomphalia: L. cinereispinula Neville & Fouchier nov. sp . Bulletin Semestriel de la Fédération des Associations Mycologiques Méditerranéennes 36: 1524.
Oberwinkler, F. (2012) Basidiolichens. In The Mycota. Fungal Associations IX, 2nd edn (B. Hock, ed.): 341362. Berlin: Springer Verlag.
Penn, O., Privman, E., Ashkenazy, H., Landan, G., Graur, D. & Pupko, T. (2010 a) GUIDANCE: a web server for assessing alignment confidence scores. Nucleic Acids Research 38: W23W28.
Penn, O., Privman, E., Landan, G., Graur, D. & Pupko, T. (2010 b) An alignment confidence score capturing robustness to guide tree uncertainty. Molecular Biology and Evolution 27: 17591767.
Poelt, J. & Oberwinkler, F. (1964) Zur Kenntnis der flechtenbildenden Blätterpilze der Gattung. Omphalina. Österreichische Botanische Zeitschrift 111: 398401.
Quélet, L. (1886) Quelques espèces critiques ou nouvelles de la flore mycologique de France. Compte Rendu de l’Association Française pour l’avancement des Sciences 24: 444453.
Redhead, S. A. (1984) Lichenized agarics: taxonomic and nomenclatural riddles. In Abstracts of the Second International Symposium on Arctic and Alpine Mycology, 26 August–2 September, 1984, Fetan, Switzerland, pp. 35–36.
Redhead, S. A. & Kuyper, T. W. (1987) Lichenized agarics: taxonomic and nomenclatural riddles. In Arctic and Alpine Mycology II (G. A. Laursen, J. F. Ammirati & S. A. Redhead, eds): 319348. New York: Plenum Publishing.
Redhead, S. A. & Kuyper, T. W. (1988) Phytoconis, the correct generic name for the basidiolichen Botrydina . Mycotaxon 31: 221223.
Redhead, S. A., Lutzoni, F. M., Moncalvo, J.-M. & Vilgalys, R. (2002) Phylogeny of agarics: partial systematics solutions for core omphalinoid genera in the Agaricales (Euagarics). Mycotaxon 83: 1957.
Saar, I. & Voitk, A. (2015) Type studies of two Tricholomopsis species described by Peck. Mycological Progress 14: 46.
Schoch, C. L., Seifert, K. A., Huhndorf, S., Robert, V., Spouge, J. L., Levesque, C. A. & Chen, W., Fungal Barcoding Consortium (2012) Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proceedings of the National Academy of Sciences of the United States of America 109: 6241–6246.
Singer, R. (1948) New and interesting species of Basidiomycetes. II. Papers of the Michigan Academy of Science, Arts and Letters 32: 103150.
Singer, R. (1952) The agarics of the Argentine sector of Tierra del Fuego I. Sydowia 6: 165226.
Singer, R. & Clémençon, H. (1972) Notes on some Leucosporous and Rhodosporous European Agarics. Nova Hedwigia 23: 305351.
Stamatakis, A. (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22: 26882690.
Sulzbacher, M. A., Wartchow, F., Ovrebo, C. L., Sousa, J. O., Baseia, I. G., Moncada, B. & Lücking, R. (2016) Sulzbacheromyces caatingae: notes on its systematics, morphology and distribution based on ITS barcoding sequences. Lichenologist 48: 6170.
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30: 27252729.
Thiers, B. (2016 continuously updated) Index Herbariorum: A global directory of public herbaria and associated staff, New York Botanical Garden’s Virtual Herbarium. http://sweetgum.nybg.org/ih/ (Accessed 5 June 2016).
Voitk, A. (2006) Three lichenomphalias from the top of Gros Morne Mountain. Mycophile 47 (5): 111.
Voitk, A. (2017) Multiclavula of NL. Omphalina 8 (1): 36.
Zoller, S. & Lutzoni, F. (2003) Slow algae, fast fungi: exceptionally high nucleotide substitution rate differences between lichenized fungi Omphalina and their symbiotic green algae Coccomyxa . Molecular Phylogenetics and Evolution 29: 629640.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

The Lichenologist
  • ISSN: 0024-2829
  • EISSN: 1096-1135
  • URL: /core/journals/lichenologist
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords

Metrics

Full text views

Total number of HTML views: 7
Total number of PDF views: 97 *
Loading metrics...

Abstract views

Total abstract views: 335 *
Loading metrics...

* Views captured on Cambridge Core between 19th September 2017 - 16th August 2018. This data will be updated every 24 hours.