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A hidden basidiolichen rediscovered: Omphalina oreades is a separate species in the genus Lichenomphalia (Basidiomycota: Agaricales: Hygrophoraceae)

Published online by Cambridge University Press:  19 September 2017

Robert LÜCKING ,
R. Greg THORN ,
Irja SAAR ,
Michele D. PIERCEY-NORMORE ,
Bibiana MONCADA ,
Jennifer DOERING ,
Henry MANN ,
Renée LEBEUF ,
Maria VOITK  and
Andrus VOITK
Robert LÜCKING
Affiliation:
Freie Universität Berlin, Königin-Luise-St 6–8, 14195 Berlin, Germany
R. Greg THORN
Affiliation:
Department of Biology, University of Western Ontario, 1151 Richmond St. N., London, Ontario, CanadaN6A 5B7
Irja SAAR
Affiliation:
Institute of Ecology and Earth Sciences, University of Tartu, Ravila St. 14A, 50411 Tartu, Estonia
Michele D. PIERCEY-NORMORE
Affiliation:
School of Science and the Environment, Grenfell Campus, Memorial University of Newfoundland, 20 University Drive, Corner Brook, Newfoundland and Labrador, CanadaA2H 5G4
Bibiana MONCADA
Affiliation:
Licenciatura en Biología, Universidad Distrital Francisco José de Caldas, Cra. 4 No. 26D-54, Bogotá D.C., Colombia; and Science & Education, The Field Museum, 1400 South Lake Shore, Chicago, IL 60605, USA
Jennifer DOERING
Affiliation:
Department of Biological Sciences, University of Manitoba, Winnipeg, Manitoba, CanadaR3T 2N2
Henry MANN
Affiliation:
School of Science and the Environment, Grenfell Campus, Memorial University of Newfoundland, 20 University Drive, Corner Brook, Newfoundland and Labrador, CanadaA2H 5G4
Renée LEBEUF
Affiliation:
775, rang du Rapide Nord, Saint-Casimir, Quebec, Canada, G0A 3L0
Maria VOITK
Affiliation:
13 Maple St., Humber Village, Newfoundland and Labrador, CanadaA2H 2N2.
Andrus VOITK*
Affiliation:
13 Maple St., Humber Village, Newfoundland and Labrador, CanadaA2H 2N2.
*
Email: seened@gmail.com
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Abstract

Molecular studies have shown the type collection of Omphalina oreades to be conspecific with a small brown basidiolichen from the Appalachian range in Newfoundland, both with 4-spored basidia. Two sequences deposited in GenBank, originally identified as O. grisella, fell in the same clade. Sequences of the type collection of Omphalia grisella, with 2-spored basidia, formed a sister clade together with two GenBank deposits, one identified as O. grisella and the other as Omphalina velutina. Omphalina oreades is recombined here as Lichenomphalia oreades comb. nov., and the species redescribed and illustrated. Sequences of the internal transcribed spacer regions of nuclear ribosomal DNA (ITS rDNA) from the algae associated with two collections of L. oreades fell within a highly supported clade with members of an undetermined species of Coccomyxa. The most abundant algal ribosomal large subunit sequence from granules at the base of a different collection matched GenBank sequences identified as Chloroidium ellipsoideum, which is probably either a free-living algal species or a partner to a species of Trapeliopsis. The second most abundant sequence matched Coccomyxa subellipsoidea and is the most likely photobiont of L. oreades. Further studies are required to elucidate the relationship of L. velutina to these taxa.

Keywords

ITS barcodingIllumina sequencingNew Hampshirephylogenytypification
Type
Articles
Information
The Lichenologist , Volume 49 , Issue 5 , September 2017 , pp. 467 - 481
Copyright
© British Lichen Society, 2017 

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