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High performance thin layer chromatography (HPTLC), an improved technique for screening lichen substances

  • Ulf Arup (a1), Stefan Ekman (a1), Louise Lindblom (a1) and Jan-Eric Mattsson (a1)
  • DOI:
  • Published online: 28 March 2007

High performance thin layer chromatography (HPTLC) is a method that can be used for screening lichen substances. It is as simple to use as standard TLC, but has many advantages: It is more sensitive, it is possible to run more samples in a shorter period of time, and the amount of solvent used is much smaller. The material needed and the methods used are described in detail. Horizontal chromatogram development was used. Since two of the solvents used in system B have been substituted, and since the properties of the HPTLC plates are slightly different, our results are not entirely in accordance with the standardized TLC method. A revised table for the identification of 69 lichen substances (obtained from 62 taxa) is accordingly presented.

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The Lichenologist
  • ISSN: 0024-2829
  • EISSN: 1096-1135
  • URL: /core/journals/lichenologist
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