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The Sticta filix morphodeme (Ascomycota: Lobariaceae) in New Zealand with the newly recognized species S. dendroides and S. menziesii: indicators of forest health in a threatened island biota?

  • Hannah RANFT (a1), Bibiana MONCADA (a2), Peter J. DE LANGE (a3), H. Thorsten LUMBSCH (a4) and Robert LÜCKING (a5)...

We present a phylogenetic revision of the Sticta filix morphodeme in New Zealand. This non-monophyletic group of early diverging clades in the genus Sticta is characterized by a stalked thallus with a green primary photobiont and the frequent formation of a dendriscocauloid cyanomorph. Traditionally, three species have been distinguished in New Zealand: S. filix (Sw.) Nyl., S. lacera (Hook. f. & Taylor) Müll. Arg. and S. latifrons A. Rich., with two cyanomorphs separated under the names Dendriscocaulon dendriothamnodes Dughi ex D. J. Galloway (traditionally associated with S. latifrons) and D. dendroides (Nyl.) R. Sant. ex H. Magn. (traditionally associated with S. filix). Sticta lacera was not included in the present study due to the lack of authentic material (all specimens originally identified under that name and sequenced clustered with S. filix); S. filix was confirmed as a distinct species whereas S. latifrons s. lat. was shown to represent two unrelated species, S. latifrons s. str. and the reinstated S. menziesii Hook. f. & Taylor. The cyanomorphs of S. filix and S. latifrons are not conspecific with the types of the names D. dendriothamnodes and D. dendroides, respectively; the D. dendriothamnodes cyanomorph belongs to the Australian taxon Sticta stipitata C. Knight ex F. Wilson, which is not present in New Zealand, whereas the D. dendroides cyanomorph corresponds to a previously unrecognized species with unknown chloromorph, recombined here as Sticta dendroides (Nyl.) Moncada, Lücking & de Lange. Thus, instead of three species (S. filix, S. lacera, S. latifrons) with their corresponding cyanomorphs, five species are now distinguished in this guild in New Zealand: S. dendroides (cyanomorph only), S. filix (chloro- and cyanomorph), S. lacera (chloromorph only), S. latifrons (chloro- and cyanomorph) and S. menziesii (chloro- and cyanomorph). A key is presented for identification of the chloromorphs and the dendriscocauloid cyanomorphs of all species. Semi-quantitative analysis suggests that species in this guild are good indicators of intact forest ecosystems in New Zealand and that the two newly recognized species, S. dendroides and S. menziesii, appear to perform particularly well in this respect. The use of lichens as bioindicators of environmental health is not yet established in New Zealand and so, based on our results, we make the case to develop this approach more thoroughly.

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Anderson, A. (2002) Faunal collapse, landscape change and settlement history in remote Oceania. World Archaeology 33: 375390.
Ariyawansa, H. A., Hawksworth, D. L., Hyde, K. D., Jones, E. B. G., Maharachchikumbura, S. S. N., Manamgoda, D. S., Thambugala, K. M., Udayanga, D., Camporesi, E., Daranagama, A., et al. (2014) Epitypification and neotypification: guidelines with appropriate and inappropriate examples. Fungal Diversity 69: 5791.
Atkinson, I. A. E. (1989) Introduced animals and extinctions. In Conservation for the Twenty-first Century (D. C. Western & M. C. Pearl, eds): 5475. New York: Oxford University Press.
Brodo, I. M., Duran Sharnoff, S. & Sharnoff, S. (2001) Lichens of North America. New Haven & London: Yale University Press.
Brown, M. A., Stephens, R. T. T., Peart, R. & Fedder, B. (2015) Vanishing Nature: Facing New Zealand’s Biodiversity Crisis. Auckland: Environmental Defence Society Inc.
Buckley, H. L., Rafat, A., Ridden, J. D., Cruickshank, R. H., Ridgway, H. J. & Paterson, A. M. (2014) Phylogenetic congruence of lichenised fungi and algae is affected by spatial scale and taxonomic diversity. Peer 2: e573 https://doi:org/10.7717/peerj.573
Campbell, J. & Fredeen, A. L. (2004) Lobaria pulmonaria abundance as an indicator of macrolichen diversity in interior cedar-hemlock forests of east-central British Columbia. Canadian Journal of Botany 82: 970982.
Cansaran-Duman, D., Altunkaynak, E., Aslan, A., Büyük, Á. & Aras, S. (2015) Application of molecular markers to detect DNA damage caused by environmental pollutants in lichen species. Genetics and Molecular Research 14: 46374650.
de Lange, P. J. & Galloway, D. J. (2015) Lichen notes from the Kermadec Islands. I. Lobariaceae. Bulletin of the Auckland . Museum 20: 141170.
de Lange, P. J., Heenan, P. B., Norton, D. A., Rolfe, J. R. & Sawyer, J. W. D. (2010) Threatened Plants of New Zealand. Christchurch: Canterbury University Press.
de Lange, P. J., Galloway, D. J., Blanchon, D. J., Knight, A., Rolfe, J. R., Crowcroft, G. M. & Hitchmough, R. (2012) Conservation status of New Zealand lichens. New Zealand Journal of Botany 50: 303363.
Drummond, A. J. & Rambaut, A. (2007) BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evolutionary Biology 7: 214.
Galloway, D. J. (1983) New taxa in the New Zealand lichen flora. New Zealand Journal of Botany 21: 191200.
Galloway, D. J. (1985) Flora of New Zealand Lichens. Wellington: P. D. Hasselberg, Government Printer.
Galloway, D. J. (1988) Studies in Pseudocyphellaria (lichens). I. The New Zealand species. Bulletin of the British Museum (Natural History), Botany Series 17: 1267.
Galloway, D. J. (1992) Lichens of Laguna San Rafael, Parque Nacional ‘Laguna san Rafael’, southern Chile: indicators of environmental change. Global Ecology and Biogeography Letters 2: 3745.
Galloway, D. J. (1997) Studies on the lichen genus Sticta (Schreber) Ach. IV. New Zealand species. Lichenologist 29: 105168.
Galloway, D. J. (2001) Lobariaceae . In Flora of Australia. Volume 58A, Lichens 3 (P. M. McCarthy, ed.): 37101. Melbourne: ABRS/CSIRO.
Galloway, D. J. (2007) Flora of New Zealand Lichens. Revised Second Edition Including Lichen-Forming and Lichenicolous Fungi. Volumes 1 and 2. Lincoln, New Zealand: Manaaki Whenua Press.
Galloway, D. J. (2008) Austral lichenology: 1690–2008. New Zealand Journal of Botany 46: 433521.
Galloway, D. J. (2009) Lichen notes 2: lichens on the move – are they telling us something? New Zealand Botanical Society Newsletter 98: 1519.
Galloway, D. J. (2015) Contributions to a history of New Zealand lichenology 5*. James Murray (1923–1961). Phytotaxa 198: 167.
Galloway, D. J. & Elix, J. A. (2013) Reinstatement of Crocodia Link (Lobariaceae, Ascomycota) for five species formerly included in Pseudocyphellaria Vain. Australasian Lichenology 72: 3242.
Galloway, D. J., Stenroos, S. & Ferraro, L. I. (1995) Flora Criptogámica de Tierra del Fuego. Lichenes, Peltigerales: Lobariaceae y Stictaceae. Vol. 6, Fasc. 6. Buenos Aires: Consejo Nacional de Investigaciones Cientificas y Técnicas de la República Argentina.
Gardes, M. & Bruns, T. D. (1993) ITS primers with enhanced specificity for basidiomycetes – application to the identification of mycorrhizae and rust. Molecular Ecology 2: 113118.
Gauslaa, Y. (1994) Lungenever, Lobaria pulmonaria, som indikator på artsrike kontinuitetsskoger. Blyttia 52: 119128.
Giorgio, B., Luisa, F. & Sonia, R. (2015) Structural variables drive the distribution of the sensitive lichen Lobaria pulmonaria in Mediterranean old-growth forests. Ecological Indicators 53: 3742.
Gustafsson, L., Fedrowitz, K. & Hazell, P. (2013) Survival and vitality of a macrolichen 14 years after transplantation on aspen trees retained at clearcutting. Forest Ecology and Management 291: 436441.
Hall, T. A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 9598.
Hayward, G. C., Blanchon, D. J. & Lumbsch, H. T. (2014) Molecular data support Ramalina ovalis as a distinct lineage (Ramalinaceae, Ascomycota). Lichenologist 46: 553561.
Hodkinson, B. P., Lendemer, J. C., McDonald, T. & Harris, R. C. (2014) The status of Sticta sylvatica, an ‘exceedingly rare’ lichen species in eastern North America. Evansia 31: 1724.
Holdaway, R. N. (1989) New Zealand’s pre-human avifauna and its vulnerability. New Zealand Journal of Ecology 12 (supplement): 1125.
Hooker, J. D. (1855) The Botany of the Antarctic Voyage of H. M. Discovery Ships Erebus and Terror 1839–1843. II. Flora Novae-Zelandiae, Part II: Flowerless Plants. London: Lovell Reeve.
Hutcheson, J., Walsh, P. & Given, D. R. (1999) Potential Value of Indicator Species for Conservation and Management of New Zealand Terrestrial Communities. Wellington: Department of Conservation.
Jaklitsch, W. M., Baral, H. O., Lücking, R. & Lumbsch, H. T. (2016) Ascomycota. In Syllabus of Plant Families. Adolf Engler’s Syllabus der Pflanzenfamilien (W. Frey, ed.): 1322. Stuttgart: Borntraeger.
James, P. W. & Henssen, A. (1976) The morphological and taxonomic significance of cephalodia. In Lichenology: Progress and Problems (D. H. Brown, D. L. Hawksworth & R. H. Bailey, eds): 2777. London: Academic Press.
Jüriado, I. & Liira, J. (2009) Distribution and habitat ecology of the threatened forest lichen Lobaria pulmonaria in Estonia. Folia Cryptogamica Estonica 46: 5565.
Kalwij, J. M., Wagner, H. H. & Scheidegger, C. (2005) Effects of stand-level disturbances on the spatial distribution of a lichen indicator. Ecological Applications 15: 20152024.
Katoh, K., Misawa, K., Kuma, K. & Miyata, T. (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research 30: 30593066.
Katoh, K., Asimenos, G. & Toh, H. (2009) Multiple alignment of DNA sequences with MAFFT. Methods in Molecular Biology 537: 3964.
King, C. M. (ed.) (1990) The Handbook of New Zealand Mammals. Auckland: Oxford University Press.
Lücking, R., Hodkinson, B. P. & Leavitt, S. D. (2017 ‘2016’) The 2016 classification of lichenized fungi in the Ascomycota and Basidiomycota – approaching one thousand genera. Bryologist 119: 361416.
Magain, N. & Sérusiaux, E. (2015) Dismantling the treasured flagship lichen Sticta fuliginosa (Peltigerales) into four species in Western Europe. Mycological Progress 14: 10.1007/s11557-015-1109-0.
Magain, N., Goffinet, B. & Sérusiaux, E. (2012) Further photomorphs in the lichen family Lobariaceae from Reunion (Mascarene archipelago) with notes on the phylogeny of Dendriscocaulon cyanomorphs. Bryologist 115: 243254.
Mark, A. F., Scott, G. A. M., Sanderson, F. R. & James, P. W. (1964) Forest succession on landslides above Lake Thomson, Fiordland. New Zealand Journal of Botany 2: 6089.
Marx, A., Backes, C., Meese, E., Lenhof, H. P. & Keller, A. (2016) EDISON-WMW: Exact dynamic programing solution of the Wilcoxon-Mann-Whitney Test. Genomics Proteomics Bioinformatics 14: 5561.
McCune, B. (2000) Lichen communities as indicators of forest health. Bryologist 103: 353356.
Moncada, B., Lücking, R. & Betancourt-Macuase, L. (2013 a) Phylogeny of the Lobariaceae (lichenized Ascomycota: Peltigerales), with a reappraisal of the genus Lobariella . Lichenologist 45: 203263.
Moncada, B., Coca, L. F. & Lücking, R. (2013 b) Neotropical members of Sticta (lichenized Ascomycota: Lobariaceae) forming photosymbiodemes, with the description of seven new species. Bryologist 116: 169200.
Moncada, B., Lücking, R. & Suárez, A. (2014 a) Molecular phylogeny of the genus Sticta (lichenized Ascomycota: Lobariaceae) in Colombia. Fungal Diversity 64: 205231.
Moncada, B., Reidy, B. & Lücking, R. (2014 b) A phylogenetic revision of Hawaiian Pseudocyphellaria sensu lato (lichenized Ascomycota: Lobariaceae) reveals eight new species and a high degree of inferred endemism. Bryologist 117: 119160.
Moncada, B., Ranft, H., de Lange, P. J., Blanchon, D., Knight, A., Lumbsch, H. T. & Lücking, R. (2016) The lichen family Lobariaceae in New Zealand: assessing traditional species concepts using the ITS barcoding locus. In Abstracts of the 8th International Association for Lichenology Symposium, 1–5 August, 2016, Helsinki, Finland, p. 132.
Myles, B. C. (2014) The evolution of a lichen symbiosis: a case study investigating Menegazzia (Ascomycota) and Trebouxia (Chlorophyta). MSc thesis, University of Otago.
Nascimbene, J., Brunialti, G., Ravera, S., Frati, L. & Caniglia, G. (2010) Testing Lobaria pulmonaria (L.) Hoffm. as an indicator of lichen conservation importance of Italian forests. Ecological Indicators 10: 353360.
Normann, F., Weigelt, P., Gehrig-Downie, C., Gradstein, S. R., Sipman, H. J. M., Obregon, A. & Bendix, J. (2010) Diversity and vertical distribution of epiphytic macrolichens in lowland rain forest and lowland cloud forest of French Guiana. Ecological Indicators 10: 11111118.
Nylander, W. (1867) Addenda quaedam ad lichenographiam Novae Zelandiae. Flora 50: 438440.
Nylander, W. (1888) Lichenes Novae Zelandiae. Paris: Schmidt.
Parnmen, S., Rangsiruji, A., Mongkolsuk, P., Boonpragob, K., Nutakki, A. & Lumbsch, H. T. (2012) Using phylogenetic and coalescent methods to understand the species diversity in the Cladia aggregata complex (Ascomycota, Lecanorales). PLoS ONE 7: e52245.
Parnmen, S., Leavitt, S. D., Rangsiruji, A. & Lumbsch, H. T. (2013) Identification of species in the Cladia aggregata group using DNA barcoding. Phytotaxa 115: 114.
Pino-Bodas, R., Burgaz, A. R., Martín, M. P., Ahti, T., Stenroos, S., Wedin, M. & Lumbsch, H. T. (2015) The phenotypic features used for distinguishing species within the Cladonia furcata complex are highly homoplasious. Lichenologist 47: 287303.
Prebble, M. & Wilmshurst, J. M. (2009) Detecting the initial impact of humans and introduced species on island environments in Remote Oceania using palaeoecology. Biological Invasions 11: 15291556.
Ramírez-Morán, N. A., León-Gómez, M. & Lücking, R. (2016) Uso de biotipos de líquenes como bioindicadores de perturbación en fragmentos de bosque altoandino (Reserva Biológica “Encenillo”, Colombia). Caldasia 38: 3152.
Rosabal, D., Burgaz, A. R. & De la Masa, R. (2010) Diversity and distribution of epiphytic macrolichens on tree trunks in two slopes of the montane rainforest of Gran Piedra, Santiago de Cuba. Bryologist 113: 313321.
Rose, F. (1974) The epiphytes of oak. In The British Oak: Its History and Natural History (M. G. Morris & F. H. Perring, eds): 250273. Faringdon: E. W. Classey.
Rose, F. (1976) Lichenological indicators of age and ecological continuity in Woodlands. In Lichenology: Progress and Problems (D. H. Brown, D. L. Hawksworth & R. H. Bailey, eds): 279307. London: Academic Press.
Rose, F. (1992) Temperate forest management: its effects on bryophyte and lichen floras and habitats. In Bryophytes and Lichens in a Changing Environment (J. W. Bates & A. M. Farmer, eds): 211233. Oxford: Oxford Scientific Publications.
Scheidegger, C. (2009) Flechten: Bioindikatoren für Veränderungen in der Umwelt. Rundgespräche der Kommission für Ökologie 36: 143159.
Schoch, C. L., Seifert, K. A., Huhndorf, S., Robert, V., Spouge, J. L., Levesque, C. A. & Chen, W., Fungal Barcoding Consortium (2012) Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi . Proceedings of the National Academy of Sciences of the United States of America 109: 62416246.
Selva, S. B. (1994) Lichen diversity and stand continuity in the northern hardwoods and spruce-fir forests of northern New England and western New Brunswick. Bryologist 97: 424429.
Selva, S. B. (1996) Using lichens to assess ecological continuity in northeastern forests. In Eastern Old-Growth Forests: Prospects for Rediscovery and Recovery (M. Byrd, ed.): 3548. Washington, D. C.: Island Press.
Simijaca-Salcedo, D. F., Vargas-Rojas, D. L. & Morales-Puentes, M. E. (2014) Use of non-vascular plant organisms as indicators of urban air pollution (Tunja, Boyacá, Colombia). Acta Biologica Colombiana 19: 221231.
Søchting, U. & Christensen, S. N. (1989) Overvagning af Laver i Danske Naturskove 1988. Horsholm: Naturovervagningsrapport, Miljoministeriet, Skovog Naturstyrelsen.
Stamatakis, A. (2015) Using RAxML to infer phylogenies. Unit 6.14. Current Protocols in Bioinformatics 51: 16.
Stenroos, S., Stocker-Wörgötter, E., Yoshimura, I., Myllys, L., Thell, A. & Hyvönen, J. (2003) Culture experiments and DNA sequence data confirm the identity of Lobaria photomorphs. Canadian Journal of Botany 81: 232247.
Stenroos, S., Velmala, S., Pykälä, J. & Ahti, T. (eds) (2016) Lichens of Finland. Norrlinia 30: 1896.
Summerfield, T. C., Galloway, D. J. & Eaton-Rye, J. J. (2002) Species of cyanolichens from Pseudocyphellaria with indistinguishable ITS sequences have different photobionts. New Phytologist 155: 121129.
Takahashi, K., Wang, L.-S., Tsubota, H. & Deguchi, H. (2006) Photosymbiodemes Sticta wrightii and Dendriscocaulon sp. (lichenized Ascomycota) from Yunnan, China. Journal of the Hattori Botanical Laboratory 100: 783796.
Tennyson, A. J. D. & Martinson, P. (2006) Extinct Birds of New Zealand. Wellington: Te Papa Press.
Thomas, M. A., Ryan, D. J., Farnden, K. J. F. & Galloway, D. J. (2002) Observations on phylogenetic relationships within Lobariaceae Chevall. (Lecanorales, Ascomycota) in New Zealand, based on ITS-5.8s molecular sequence data. Bibliotheca Lichenologica 82: 123138.
Tønsberg, T. & Goward, T. (2001) Sticta oroborealis sp. nov. and other Pacific North American lichens forming dendriscocauloid cyanotypes. Bryologist 104: 1223.
Tønsberg, T., Blom, H. H., Goffinet, B., Holtan-Hartwig, J. & Lindblom, L. (2016) The cyanomorph of Ricasolia virens comb. nov. (Lobariaceae, lichenized Ascomycetes). Opuscula Philolichenum 15: 1221.
White, T. J., Bruns, T. D., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols. A Guide to Methods and Applications (M. A. Innis, D. H. Gelfand, J. J. Sninsky & T. J. White, eds): 315322. San Diego: Academic Press.
Wirth, V., Hauck, M. & Schultz, M. (2013) Die Flechten Deutschlands, 2 Volumes. Stuttgart: Ulmer.
Wolseley, P. A., Moncrieff, C. & Aguirre-Hudson, B. (1994) Lichens as indicators of environmental stability and change in the tropical forests of Thailand. Global Ecology and Biogeography Letters 4: 116123.
Worthy, T. H. & Holdaway, R. N. (2002) The Lost World of the Moa. Christchurch: Canterbury University Press.
Zedda, L. (2002) The epiphytic lichens on Quercus in Sardinia (Italy) and their value as ecological indicators. Englera 24: 1468.
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