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Thamnolia tundrae sp. nov., a cryptic species and putative glacial relict

  • Ioana ONUT-BRÄNNSTRÖM (a1), Hanna JOHANNESSON (a1) and Leif TIBELL (a1)

Abstract

The lichen species of the genus Thamnolia, with their striking wormlike thalli and frequent occurrence in arctic and tundra environments, have often been debated with regard to the use of chemistry in lichen taxonomy. Phylogenetic studies have arrived at different conclusions as to the recognition of species in the genus, but in a recent study based on the analyses of six nuclear markers (genes or noncoding regions) of a worldwide sample of Thamnolia, we showed the existence of three well-supported lineages with two different chemistries and geographical distributions. Here, we present two analyses based on ITS and three markers, respectively, which were extended from the study mentioned above to include type specimens and additional Thamnolia strains and taxa. In these analyses the same three clades were retrieved. A putative DEAD-box helicase is used here for the first time as an informative phylogenetic marker to provide taxonomic resolution at species level. The distribution of morphological and chemical characters across the phylogeny was analyzed and it was concluded that three morphologically cryptic, but genetically well supported, species occur: T. vermicularis s. str., T. subuliformis s. str. and T. tundrae sp. nov. Thamnolia vermicularis s. str. contains individuals with uniform secondary chemistry (producing thamnolic acid) and a rather limited distribution in the European Alps, Tatra Mts and the Western Carpathians, a distribution which might result from glacial survival in an adjacent refugium/refugia. Thamnolia subuliformis s. str. is widely distributed in all hemispheres and the samples contain two chemotypes (either with thamnolic or squamatic acids). Thamnolia tundrae is described as new; it produces baeomycesic and squamatic acids, and has a distribution limited to the arctic tundra of Eurasia extending to the Aleutian Islands in North America. It may have survived the latest glaciation in coastal refugia near its present distribution. Thus, secondary chemistry alone is not suitable for characterizing species in Thamnolia, secondary chemistry and geographical origin are informative, and the ITS region can be confidently used for species recognition. Nomenclatural notes are given on several other names that have been used in Thamnolia.

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Andrei, M., Iacob, M. & Pascale, M. (2006–2007) Vegetative multiplication in Thamnolia vermicularis (Sw.) Schaer. Romanian Journal of Biology, Plant Biology 51-52: 5557.
Bryant, D. & Moulton, V. (2004) Neighbor-Net: an agglomerative method for the construction of phylogenetic networks. Molecular Biology and Evolution 21: 255265.
Crespo, A. & Lumbsch, H. T. (2010) Cryptic species in lichen-forming fungi. IMA Fungus 1: 167170.
Culberson, W. L. (1963) The lichen genus Thamnolia . Brittonia 15: 140144.
Darriba, D., Taboada, G., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9: 772.
Huson, D. H. & Bryant, D. (2006) Application of phylogenetic networks in evolutionary studies. Molecular Biology and Evolution 23: 254267.
Kashiwandani, H. & Kurokawa, S. (2003) Index of Type Specimens of Lichens Preserved in the National Science Museum, Tokyo. Tokyo: National Science Museum.
Katoh, S. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30: 772780.
Kärnefelt, E. I. & Thell, A. (1995) Genotypical variation and reproduction in natural populations of Thamnolia. Bibliotheca Lichenologica 58: 213243.
Larsson, A. (2014) AliView: a fast and lightweight alignment viewer and editor for large data sets. Bioinformatics 30: 32763278.
Leavitt, S. D., Moreau, C. S. & Lumbsch, H. T. (2015) The dynamic discipline of species delimitation: progress toward effectively recognizing species boundaries in natural populations. In Recent Advances in Lichenology (D. K. Upreti, P. K. Divakar, V. Shukla & R. Bajpai, eds): 1144. New Delhi: Springer India.
Leavitt, S. D., Divakar, P. K., Crespo, A. & Lumbsch, H. T. (2016) A matter of time – understanding the limits of the power of molecular data for delimiting species boundaries. Herzogia 29: 479492.
Lord, J. M., Knight, A., Bannister, J. M., Ludwig, L. R., Malcolm, W. M. & Orlovich, D. A. (2013) Rediscovery of pycnidia in Thamnolia vermicularis: implications for chemotype occurrence and distribution. Lichenologist 45: 397411.
Lumbsch, H. T. & Leavitt, S. D. (2011) Goodbye morphology? A paradigm shift in the delimitation of species in lichenized fungi. Fungal Diversity 50: 5972.
Minks, A. (1874) Thamnolia vermicularis. Eine Monographie. Flora 57: 337347.
Molina, C., Del-Prado, R. & Kumar, P. (2011) Another example of cryptic diversity in lichen-forming fungi: the new species Parmelia mayi (Ascomycota: Parmeliaceae). Organisms Diversity and Evolution 11: 331342.
Nelsen, M. P. & Gargas, A. (2009) Assessing clonality and chemotype monophyly in Thamnolia (Icmadophilaceae). Bryologist 112: 4253.
Onuţ-Brännström, I., Tibell, L. & Johannesson, H. (2017) A worldwide phylogeography of the whiteworm lichens Thamnolia reveals three lineages with distinct habitats and evolutionary histories. Ecology and Evolution 7: 36023615.
Platt, J. L. & Spatafora, J. W. (2000) Evolutionary relationships of nonsexual lichenized fungi: molecular phylogenetic hypotheses for the genera Siphula and Thamnolia from SSU and LSU rDNA. Mycologia 92: 475487.
Rambaut, A., Suchard, M., Xie, D. & Drummond, A. (2014) Tracer v1.6. Available from http://beast.bio.ed.ac.uk/Tracer.
Redelings, B. (2014) Erasing errors due to alignment ambiguity when estimating positive selection. Molecular Biology and Evolution 31: 19791993.
Santesson, R. (2004) Two new species of Thamnolia . Symbolae Botanicae Upsalienses 34 (1): 393397.
Satô, M. (1965) The mixture ratio of the lichen genus Thamnolia in New Zealand. Bryologist 68: 320324.
Sheard, J. W. (1977) Paleogeography, chemistry and taxonomy of the lichenized Ascomycetes Dimelaena and Thamnolia . Bryologist 80: 100118.
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30: 27252729.
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The Lichenologist
  • ISSN: 0024-2829
  • EISSN: 1096-1135
  • URL: /core/journals/lichenologist
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