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Untangling the hidden intrathalline microalgal diversity in Parmotrema pseudotinctorum: Trebouxia crespoana sp. nov.

Published online by Cambridge University Press:  08 May 2018

Pavel ŠKALOUD ,
Patricia MOYA ,
Arántzazu MOLINS ,
Ondřej PEKSA ,
Arnoldo SANTOS-GUERRA  and
Eva BARRENO
Pavel ŠKALOUD
Affiliation:
Department of Botany, Faculty of Sciences, Charles University, Benátská 2, 12800-Prague, Czech Republic. Email: skaloud@natur.cuni.cz
Patricia MOYA
Affiliation:
ICBIBE, Dpto. Botánica, Facultat de Ciències Biològiques, Universitat de València, C/ Dr. Moliner 50, 46100-Burjassot, València, Spain
Arántzazu MOLINS
Affiliation:
ICBIBE, Dpto. Botánica, Facultat de Ciències Biològiques, Universitat de València, C/ Dr. Moliner 50, 46100-Burjassot, València, Spain
Ondřej PEKSA
Affiliation:
The West Bohemian Museum in Pilsen, Kopeckého sady 2, 30100-Plzeň, Czech Republic
Arnoldo SANTOS-GUERRA
Affiliation:
Calle Guaidil 16, 38280 Tegueste, Tenerife, Spain
Eva BARRENO
Affiliation:
ICBIBE, Dpto. Botánica, Facultat de Ciències Biològiques, Universitat de València, C/ Dr. Moliner 50, 46100-Burjassot, València, Spain
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Abstract

Intrathalline phycobiont diversity was investigated in a rosette-forming lichen, Parmotrema pseudotinctorum, using a combination of Sanger sequencing, 454-pyrosequencing, conventional light and confocal microscopy, and transmission electron microscopy. A total of 39 thalli sampled in five Canary Island populations were investigated. Three novel lineages of lichen phycobionts were detected, all being inferred within the Trebouxia clade G. The most abundant phycobiont lineage, occurring in all lichen populations investigated, is described here as Trebouxia crespoana sp. nov. This species produces spherical to pyriform cells possessing a crenulate chloroplast with lobes elongated at their ends, and one corticola-type pyrenoid with very thin, unbranched tubules of curved profile. Trebouxia crespoana is clearly distinguished from all other Trebouxia species by a characteristic cap-like cell wall thickening produced on one side of vegetative cells, and the larger size of vegetative cells that reach 21(–26) µm in diameter.

Keywords

454-pyrosequencingCanary Islandslichensmorphologyphycobiontsultrastructure
Type
Articles
Information
The Lichenologist , Volume 50 , Issue 3 , May 2018 , pp. 357 - 369
Copyright
© British Lichen Society, 2018 

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