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Discrimination between Gyrodactylus salaris, G. derjavini and G. truttae (Platyhelminthes: Monogenea) using restriction fragment length polymorphisms and an oligonucleotide probe within the small subunit ribosomal RNA gene

Published online by Cambridge University Press:  06 April 2009

C. O. Cunningham ,
D. M. McGillivray ,
K. MacKenzie  and
W. T. Melvin
C. O. Cunningham
Affiliation:
SOAFD Marine Laboratory, P.O. Box 101, Victoria Road, Aberdeen AB9 8DB
K. MacKenzie
Affiliation:
SOAFD Marine Laboratory, P.O. Box 101, Victoria Road, Aberdeen AB9 8DB
W. T. Melvin
Affiliation:
Department of Molecular and Cell Biology, University of Aberdeen, Aberdeen AB9 1AS
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Summary

The small subunit ribosomal RNA (srRNA) gene was amplified from Gyrodactylus salaris using the polymerase chain reaction (PCR), cloned, and the complete gene sequence of 1966 bp determined. The V4 region of the srRNA gene was identified and amplified from single specimens of G. salaris, G. derjavini and G. truttae. Comparison of the V4 sequences from these three species revealed sequence differences from which restriction fragment length polymorphisms (RFLPs) were predicted and an oligonucleotide probe (GsV4) specific to G. salaris designed. Digestion of the amplified V4 region of the srRNA gene with Hae III and either Alw I, BstY I, Dde I or Mbo I provided a means of discriminating between G. salaris, G. derjavini and G. truttae. The GsV4 probe was used to detect the srRNA gene from G. salaris in Southern and dot blots of the amplified V4 region. The nucleotide sequences reported in this paper have been submitted to the EMBL Data Library under accession numbers Z26942 (G. salaris), Z35128 (G. derjavini) and Z35129 (G. truttae).

Keywords

Gyrodactylussmall subunit rRNAPCRRFLPoligonucleotide probe.
Type
Research Article
Information
Parasitology , Volume 111 , Issue 1 , July 1995 , pp. 87 - 94
Copyright
Copyright © Cambridge University Press 1995

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