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Flow cytometry analysis of the circulating haemocytes from Biomphalaria glabrata and Biomphalaria tenagophila following Schistosoma mansoni infection

Published online by Cambridge University Press:  07 January 2009

R. L. MARTINS-SOUZA ,
C. A. J. PEREIRA ,
P. M. Z. COELHO ,
O. A. MARTINS-FILHO  and
D. NEGRÃO-CORRÊA
R. L. MARTINS-SOUZA
Affiliation:
Departamento de Parasitologia, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), Belo Horizonte – MG. Brasil
C. A. J. PEREIRA
Affiliation:
Departamento de Parasitologia, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), Belo Horizonte – MG. Brasil
P. M. Z. COELHO
Affiliation:
Centro de Pesquisas René Rachou (CPRR-Fiocruz), Belo Horizonte, Minas Gerais, Brasil
O. A. MARTINS-FILHO
Affiliation:
Centro de Pesquisas René Rachou (CPRR-Fiocruz), Belo Horizonte, Minas Gerais, Brasil
D. NEGRÃO-CORRÊA*
Affiliation:
Departamento de Parasitologia, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), Belo Horizonte – MG. Brasil
*
*Corresponding author: Departamento de Parasitologia, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), Av. Presidente Antônio Carlos 6627, Campus Pampulha, 31270-901, Belo Horizonte, MG, Brasil. Tel: +55 31 3409 2855. Fax: +55 31 3409 2970. E-mail: denegrao@icb.ufmg.br
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Summary

Aiming to further characterize the haemocyte subsets in Biomphalaria snails, we have performed a detailed flow cytometric analysis of whole haemolymph cellular components using a multiparametric dual colour labelling procedure. Ethidium bromide/acridine orange fluorescence features were used to first select viable haemocytes followed by flow cytometric morphometric analysis based on the laser scatter properties (forward scatter-FSC and side scatter-SSC). Our findings demonstrated that B. glabrata (BG-BH, highly susceptible to S. mansoni) and 2 strains of B. tenagophila (BT-CF, moderately susceptible and BT-Taim, resistant to S. mansoni) have 3 major circulating haemocyte subsets, referred to as small, medium and large haemocytes. The frequency of small haemocytes was higher in BG-BH, while medium haemocytes were the most abundant cell-type in both B. tenagophila strains. Schistosoma mansoni infection resulted in early reduction of large and medium circulating haemocytes followed by an increase of small haemocytes. Although parasite infection induced haemocyte alterations in all Biomphalaria strains, the response was particularly intense in BT-Taim, the parasite-resistant snail. Interestingly, the trematode infection induces changes in haemocytes with less granular rather than in those with more granular profile. The results indicated that, in B. tenagophila of Taim strain, circulating haemocytes, especially the medium and high subset with less granular profile, are very reactive cells upon S. mansoni infection, suggesting that this cell subset would participate in the early parasite destruction observed in this snail strain.

Keywords

BiomphalariaSchistosoma mansonihaemocyteflow cytometrydefence systemmolluscs
Type
Research Article
Information
Parasitology , Volume 136 , Issue 1 , January 2009 , pp. 67 - 76
Copyright
Copyright © 2009 Cambridge University Press

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