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Leishmania donovani complex: genotyping with the ribosomal internal transcribed spacer and the mini-exon

Published online by Cambridge University Press:  03 March 2004

I. L. MAURICIO
Affiliation:
Pathogen Molecular Biology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
J. R. STOTHARD
Affiliation:
Pathogen Molecular Biology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK. Current address: Department of Infectious Disease Epidemiology, Imperial College, Norfolk Place, Paddington, London W2 1PG, UK.
M. A. MILES
Affiliation:
Pathogen Molecular Biology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.

Abstract

Intergenic region typing by restriction analysis of the ribosomal internal transcribed spacer (ITS) and mini-exon provide diagnostic markers for some Leishmania. Here, we evaluate restriction analysis of these targets for genotyping and phylogenetic analysis within the Leishmania donovani complex (agents of visceral leishmaniasis). Each method was useful for genotyping of both L. donovani complex strains and Old World Leishmania species. The targets produced less robust groups than gp63 intergenic regions, but support the need for re-evaluation of the taxonomy of the L. donovani complex.

Type
Research Article
Copyright
2004 Cambridge University Press

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References

REFERENCES

CUPOLILLO, E., GRIMALDI JUNIOR, G., MOMEN, H. & BEVERLEY, S. M. (1995). Intergenic region typing (IRT): a rapid molecular approach to the characterization and evolution of Leishmania. Molecular and Biochemical Parasitology 73, 145155.CrossRefGoogle Scholar
EL TAI, N. O., EL FARI, M., MAURICIO, I. L., MILES, M. A., OSKAM, L., EL SAFI, S. H., PRESBER, W. H. & SCHOENIAN, G. (2001). Leishmania donovani: intra-specific polymorphisms of Sudanese isolates revealed by PCR-based analyses and DNA sequencing. Experimental Parasitology 97, 3544.CrossRefGoogle Scholar
FELSENSTEIN, J. (1993). PHYLIP (Phylogeny Inference Package). Seattle, Distributed by the author. Department of Genetics, University of Washington.
FERNANDES, O., MURTHY, V. K., KURATH, U., DEGRAVE, W. M. & CAMPBELL, D. A. (1994). Mini-exon gene variation in human pathogenic Leishmania species. Molecular and Biochemical Parasitology 66, 261271.CrossRefGoogle Scholar
HARRIS, E., KROPP, G., BELLI, A., RODRIGUEZ, B. & AGABIAN, N. (1998). Single-step multiplex PCR assay for characterization of New World Leishmania complexes. Journal of Clinical Microbiology 36, 19891995.Google Scholar
HASSAN, M. Q., GHOSH, A., GHOSH, S. S., GUPTA, M., BASU, D., MALLIK, K. K. & ADHYA, S. (1993). Enzymatic amplification of mini-exon-derived RNA gene spacers of Leishmania donovani: primers and probes for DNA diagnosis. Parasitology 107, 509517.CrossRefGoogle Scholar
KATAKURA, K., KAWAZU, S., NAYA, T., NAGAKURA, K., ITO, M., AIKAWA, M., QU, J. Q., GUAN, L. R., ZUO, X. P., CHAI, J. J., CHANG, K. P. & MATSUMOTO, Y. (1998). Diagnosis of kala-azar by nested PCR based on amplification of the Leishmania mini-exon gene. Journal of Clinical Microbiology 36, 21732177.Google Scholar
KELLY, J. M. (1993). Isolation of RNA and DNA from Leishmania. In Methods in Molecular Biology (ed. Hyde, J. E.) 21, 123131. Humana Press, New Jersey.CrossRef
MAURICIO, I. L., GAUNT, M. W., STOTHARD, J. R. & MILES, M. A. (2001). Genetic typing and phylogenetic analysis of the Leishmania donovani complex by restriction analysis of PCR amplified gp63 intergenic regions. Parasitology 122, 393403.Google Scholar
RAMOS, A., MASLOV, D. A., FERNANDES, O., CAMPBELL, D. A. & SIMPSON, L. (1996). Detection and identification of human pathogenic Leishmania and Trypanosoma species by hybridization of PCR-amplified mini-exon repeats. Experimental Parasitology 82, 242250.CrossRefGoogle Scholar
RIOUX, J. A., LANOTTE, G., SERRES, E., PRATLONG, F., BASTIEN, P. & PERIERES, J. (1990). Taxonomy of Leishmania. Use of isoenzymes. Suggestions for a new classification. Annales de Parasitologie Humaine et Comparée 65, 111125.CrossRefGoogle Scholar
SCHONIAN, G., EL FARI, M., LEWIN, S., SCHWEYNOCH, C. & PRESBER, W. (2001). Molecular epidemiology and population genetics in Leishmania. Medical Microbiology and Immunology 190, 6163.CrossRefGoogle Scholar
STOTHARD, J. R., HUGHES, S. & ROLLINSON, D. (1996). Variation within the internal transcribed spacer (ITS) of ribosomal DNA genes of intermediate snail hosts within the genus Bulinus (Gastropoda: Planorbidae). Acta Tropica 61, 1929.CrossRefGoogle Scholar
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